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We performed a meta-analysis using RNA-sequencing transcriptomic datasets to identify genes involved in post-MI ECM turnover. Eight studies available in Gene Expression Omnibus were selected following the inclusion criteria. We compare RNA-sequencing data from 92 mice submitted to permanent coronary ligation or sham, identifying differentially expressed genes (p-value &lt; 0.05 and Log2FoldChange \u2265 2). Functional enrichment analysis was performed based on Gene Ontology biological processes (BPs). BPs implicated in response to extracellular stimulus, regulation of ECM organization, and ECM disassembly were detected soon after ischemia onset. ECM disassembly occurred between days one to seven post-MI, compared with ECM assembly from day seven onwards. We identified altered mRNA expression of 19 matrix metalloproteinases and four tissue inhibitors of metalloproteinases at post-infarcted ECM remodeling and altered transcriptomic expression of 42 genes encoding 26 collagen subunits at the fibrotic stage. To our knowledge, this is the first meta-analysis using RNA-sequencing datasets to evaluate post-infarcted cardiac interstitium healing, revealing previously unknown mechanisms and molecules actively implicated in ECM remodeling post-MI, which warrant further validation.<\/jats:p>","DOI":"10.3390\/ijms232415615","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T06:14:00Z","timestamp":1670566440000},"page":"15615","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database"],"prefix":"10.3390","volume":"23","author":[{"given":"Mar\u00eda","family":"Ortega","sequence":"first","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6405-6279","authenticated-orcid":false,"given":"C\u00e9sar","family":"R\u00edos-Navarro","sequence":"additional","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3483-7066","authenticated-orcid":false,"given":"Jose","family":"Gavara","sequence":"additional","affiliation":[{"name":"Centro de Biomateriales e Ingenier\u00eda Tisular, Universidad Polit\u00e9cnica de Valencia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"de Dios","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Valencia,46010 Valencia, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER)-CV, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nerea","family":"Perez-Sol\u00e9","sequence":"additional","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5565-5547","authenticated-orcid":false,"given":"Victor","family":"Marcos-Garc\u00e9s","sequence":"additional","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"},{"name":"Cardiology Department, Hospital Cl\u00ednico Universitario, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Ferr\u00e1ndez-Izquierdo","sequence":"additional","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"},{"name":"Department of Pathology, University of Valencia, 46010 Valencia, Spain"},{"name":"Pathology Department, Hospital Cl\u00ednico Universitario, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente","family":"Bod\u00ed","sequence":"additional","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"},{"name":"Department of Medicine, University of Valencia,46010 Valencia, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER)-CV, 28029 Madrid, Spain"},{"name":"Cardiology Department, Hospital Cl\u00ednico Universitario, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amparo","family":"Ruiz-Saur\u00ed","sequence":"additional","affiliation":[{"name":"INCLIVA health Research Institute, 46010 Valencia, Spain"},{"name":"Department of Pathology, University of Valencia, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1161\/CIRCRESAHA.119.311148","article-title":"The extracellular matrix in ischemic and nonischemic heart failure","volume":"125","author":"Frangogiannis","year":"2019","journal-title":"Circ. 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