{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:51:50Z","timestamp":1784083910859,"version":"3.55.0"},"reference-count":62,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T00:00:00Z","timestamp":1670630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2143267"],"award-info":[{"award-number":["2143267"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"],"award-info":[{"award-number":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["NIH HD22486"],"award-info":[{"award-number":["NIH HD22486"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009560","name":"American Gastroenterological Association","doi-asserted-by":"publisher","award":["2143267"],"award-info":[{"award-number":["2143267"]}],"id":[{"id":"10.13039\/100009560","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009560","name":"American Gastroenterological Association","doi-asserted-by":"publisher","award":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"],"award-info":[{"award-number":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"]}],"id":[{"id":"10.13039\/100009560","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009560","name":"American Gastroenterological Association","doi-asserted-by":"publisher","award":["NIH HD22486"],"award-info":[{"award-number":["NIH HD22486"]}],"id":[{"id":"10.13039\/100009560","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2143267"],"award-info":[{"award-number":["2143267"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"],"award-info":[{"award-number":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH HD22486"],"award-info":[{"award-number":["NIH HD22486"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Michigan State University","award":["2143267"],"award-info":[{"award-number":["2143267"]}]},{"name":"Michigan State University","award":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"],"award-info":[{"award-number":["2019 AGA-Allergan Foundation Pilot Research Award in Irritable Bowel Syndrome"]}]},{"name":"Michigan State University","award":["NIH HD22486"],"award-info":[{"award-number":["NIH HD22486"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The receptor tyrosine kinase Ret plays a critical role in regulating enteric nervous system (ENS) development. Ret is important for proliferation, migration, and survival of enteric progenitor cells (EPCs). Ret also promotes neuronal fate, but its role during neuronal differentiation and in the adult ENS is less well understood. Inactivating RET mutations are associated with ENS diseases, e.g., Hirschsprung Disease, in which distal bowel lacks ENS cells. Zebrafish is an established model system for studying ENS development and modeling human ENS diseases. One advantage of the zebrafish model system is that their embryos are transparent, allowing visualization of developmental phenotypes in live animals. However, we lack tools to monitor Ret expression in live zebrafish. Here, we developed a new BAC transgenic line that expresses GFP under the ret promoter. We find that EPCs and the majority of ENS neurons express ret:GFP during ENS development. In the adult ENS, GFP+ neurons are equally present in females and males. In homozygous mutants of ret and sox10\u2014another important ENS developmental regulator gene\u2014GFP+ ENS cells are absent. In summary, we characterize a ret:GFP transgenic line as a new tool to visualize and study the Ret signaling pathway from early development through adulthood.<\/jats:p>","DOI":"10.3390\/ijms232415667","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T01:42:27Z","timestamp":1670809347000},"page":"15667","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A New Transgenic Tool to Study the Ret Signaling Pathway in the Enteric Nervous System"],"prefix":"10.3390","volume":"23","author":[{"given":"Ashoka","family":"Bandla","sequence":"first","affiliation":[{"name":"Department of Integrative Biology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Melancon","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4167-2546","authenticated-orcid":false,"given":"Charlotte R.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA"},{"name":"Department of Biochemistry & Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann E.","family":"Davidson","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith S.","family":"Eisen","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Ganz","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/dvdy.24597","article-title":"Gut feelings: Studying enteric nervous system development, function, and disease in the zebrafish model system","volume":"247","author":"Ganz","year":"2018","journal-title":"Dev. 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Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/24\/15667\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:37:43Z","timestamp":1760146663000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/24\/15667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,10]]},"references-count":62,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232415667"],"URL":"https:\/\/doi.org\/10.3390\/ijms232415667","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints202206.0165.v1","asserted-by":"object"}]},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,10]]}}}