{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T02:01:00Z","timestamp":1784599260528,"version":"3.55.0"},"reference-count":34,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Candida tropicalis is an emerging pathogen with a high mortality rate due to its virulence factors, including biofilm formation, that has important repercussions on the public health system. The ability of C. tropicalis to form biofilms, which are potentially more resistant to antifungal drugs and the consequent increasing antimicrobial resistance, highlights an urgent need for the development of novel antifungal. The present study analyzed the antibiofilm capacity of the arylamidine T-2307 on two strains of Candida tropicalis. Antimicrobial activity and time-killing assays were performed to evaluate the anticandidal effects of T-2307, the antibiofilm ability on biomass inhibition and eradication was evaluated by the crystal violet (CV) method. Furthermore, in Galleria mellonella infected larvae an increased survival after pre\u2014and post- treatment with T-2307 was observed. The MTT test was used to determine the viability of immortalized human prostate epithelial cells (PNT1A) after exposure to different concentrations of T-2307. Levels of interleukin IL-4, IL-8, IL-10 were quantified after Candida infection of PNT1A cells and treatment. Active doses of T-2307 did not affect the viability of PNT1A cells, and drug concentrations of 0.005 or 0.01 \u00b5g mL\u22121 inhibited the secretion of inflammatory cytokines. Taken together, these results provide new information on T-2307, indicating this drug as a new and promising alternative therapeutic option for the treatment of Candida infections.<\/jats:p>","DOI":"10.3390\/ijms232416042","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T04:54:13Z","timestamp":1671425653000},"page":"16042","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Arylamidine T-2307 as a Novel Treatment for the Prevention and Eradication of Candida tropicalis Biofilms"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4981-8837","authenticated-orcid":false,"given":"Angela","family":"Maione","sequence":"first","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6108-9457","authenticated-orcid":false,"given":"Alessandra","family":"La Pietra","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9041-9056","authenticated-orcid":false,"given":"Antonietta","family":"Siciliano","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1156-8107","authenticated-orcid":false,"given":"Aldo","family":"Mileo","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"De Falco","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"},{"name":"National Institute of Biostructures and Biosystems (INBB), 00136 Rome, Italy"},{"name":"Center for Studies on Bioinspired Agro-Environmental Technology (BAT Center), 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5874-3961","authenticated-orcid":false,"given":"Elisabetta","family":"de Alteriis","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6805-0408","authenticated-orcid":false,"given":"Marco","family":"Guida","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8116-0839","authenticated-orcid":false,"given":"Emilia","family":"Galdiero","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples \u2018Federico II\u2019, Via Cinthia, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/jam.14949","article-title":"Biofilm of Candida albicans: Formation, regulation and resistance","volume":"131","author":"Pereira","year":"2021","journal-title":"J. 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