{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T03:41:52Z","timestamp":1789962112374,"version":"4.0.1"},"reference-count":39,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grant Agency of the Czech Republic","award":["GA-22-30494S"],"award-info":[{"award-number":["GA-22-30494S"]}]},{"name":"Grant Agency of the Czech Republic","award":["86652036"],"award-info":[{"award-number":["86652036"]}]},{"name":"Grant Agency of the Czech Republic","award":["CZ.1.05\/1.1.00\/02.0109"],"award-info":[{"award-number":["CZ.1.05\/1.1.00\/02.0109"]}]},{"name":"Grant Agency of the Czech Republic","award":["MH CZ-DRO-VFN 64165"],"award-info":[{"award-number":["MH CZ-DRO-VFN 64165"]}]},{"name":"Institute of Biotechnology RVO","award":["GA-22-30494S"],"award-info":[{"award-number":["GA-22-30494S"]}]},{"name":"Institute of Biotechnology RVO","award":["86652036"],"award-info":[{"award-number":["86652036"]}]},{"name":"Institute of Biotechnology RVO","award":["CZ.1.05\/1.1.00\/02.0109"],"award-info":[{"award-number":["CZ.1.05\/1.1.00\/02.0109"]}]},{"name":"Institute of Biotechnology RVO","award":["MH CZ-DRO-VFN 64165"],"award-info":[{"award-number":["MH CZ-DRO-VFN 64165"]}]},{"name":"BIOCEV","award":["GA-22-30494S"],"award-info":[{"award-number":["GA-22-30494S"]}]},{"name":"BIOCEV","award":["86652036"],"award-info":[{"award-number":["86652036"]}]},{"name":"BIOCEV","award":["CZ.1.05\/1.1.00\/02.0109"],"award-info":[{"award-number":["CZ.1.05\/1.1.00\/02.0109"]}]},{"name":"BIOCEV","award":["MH CZ-DRO-VFN 64165"],"award-info":[{"award-number":["MH CZ-DRO-VFN 64165"]}]},{"name":"Ministry of Health of the Czech Republic","award":["GA-22-30494S"],"award-info":[{"award-number":["GA-22-30494S"]}]},{"name":"Ministry of Health of the Czech Republic","award":["86652036"],"award-info":[{"award-number":["86652036"]}]},{"name":"Ministry of Health of the Czech Republic","award":["CZ.1.05\/1.1.00\/02.0109"],"award-info":[{"award-number":["CZ.1.05\/1.1.00\/02.0109"]}]},{"name":"Ministry of Health of the Czech Republic","award":["MH CZ-DRO-VFN 64165"],"award-info":[{"award-number":["MH CZ-DRO-VFN 64165"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. This was a cohort study of patients younger than 40 years with planned ICSI, the timing of which was determined by MS status, compared with those without MS evaluation. The angle between PB and MS and MS visibility were evaluated by optical microscope with polarizing filter. Oocytes with MS evaluation were fertilized according to MS status either 5\u20136 h after ovum pick-up (OPU) or 7\u20138 h after OPU. Oocytes without MS evaluation were all fertilized 5\u20136 h after OPU. For patients over 35 years visualization of MS influenced pregnancy rate (PR): 182 patients with MS visualization had 32% PR (58\/182); while 195 patients without MS visualization had 24% PR (47\/195). For patients under 35 years, visualization of MS did not influence PR: 140 patients with MS visualization had 41% PR (58\/140), while 162 patients without MS visualization had 41% PR (66\/162). Visualization of MS therefore appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.<\/jats:p>","DOI":"10.3390\/ijms24010105","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T06:11:24Z","timestamp":1671603084000},"page":"105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Timing of ICSI with Respect to Meiotic Spindle Status"],"prefix":"10.3390","volume":"24","author":[{"given":"Olga","family":"Tepla","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zinovij","family":"Topurko","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Jirsova","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Moosova","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Fajmonova","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radek","family":"Cabela","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6837-2148","authenticated-orcid":false,"given":"Katerina","family":"Komrskova","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic"},{"name":"Department of Zoology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6633-9432","authenticated-orcid":false,"given":"Irena","family":"Kratochvilova","sequence":"additional","affiliation":[{"name":"Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaromir","family":"Masata","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology First Faculty of Medicine, Charles University and General University Hospital in Prague, Apolinarska 18, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1080\/14647273.2018.1546411","article-title":"Knowledge and decision making about future fertility and oocyte cryopreservation among young women","volume":"24","author":"Stevenson","year":"2021","journal-title":"Hum. Fertil."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1093\/humrep\/deg274","article-title":"Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI","volume":"18","author":"Reinzi","year":"2003","journal-title":"Hum. Reprod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"997","DOI":"10.3109\/09513590.2013.822063","article-title":"Conventional IVF as a laboratory strategy to rescue fertility potential in severe poor responder patients: The impact of reproductive aging","volume":"29","author":"Artini","year":"2013","journal-title":"Gynecol. Endocrinol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1007\/s10815-022-02565-2","article-title":"From glass to life: A commentary on the assessment of the reproductive potential of cryopreserved human oocytes","volume":"39","author":"Plancha","year":"2022","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1093\/humrep\/dey067","article-title":"Is there a relation between the time to ICSI and the reproductive outcomes?","volume":"33","author":"Pujol","year":"2018","journal-title":"Hum. Reprod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2318","DOI":"10.1093\/humrep\/det278","article-title":"Assisted reproductive technology in Europe, 2009: Results generated from European registers by ESHRE","volume":"28","author":"Ferraretti","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2654","DOI":"10.1001\/jama.2015.17296","article-title":"Live-Birth Rate Associated With Repeat In Vitro Fertilization Treatment Cycles","volume":"314","author":"Smith","year":"2015","journal-title":"JAMA J. Am. Med. Assoc."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Peinado, I., Moya, I., Saez-Espinosa, P., Barrera, M., Garcia-Valverde, L., Frances, R., Torres, P., and Gomez-Torres, M.J. (2021). Impact of Maturation and Vitrification Time of Human GV Oocytes on the Metaphase Plate Configuration. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031125"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S1472-6483(10)60261-1","article-title":"Relationship between visualization of meiotic spindle in human oocytes and ICSI outcomes: A meta-analysis","volume":"18","author":"Petersen","year":"2009","journal-title":"Reprod. Biomed. Online"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S1472-6483(10)60940-6","article-title":"Meiotic spindle visualization in living human oocytes","volume":"10","author":"Rienzi","year":"2005","journal-title":"Reprod. Biomed. Online"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1038\/srep29204","article-title":"Tropomodulin-3 is essential in asymmetric division during mouse oocyte maturation","volume":"6","author":"Jo","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.rbmo.2018.12.001","article-title":"Towards the global coverage of a unified registry of IVF outcomes","volume":"38","author":"Fauser","year":"2019","journal-title":"Reprod. Biomed. Online"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1093\/humrep\/des200","article-title":"The oocyte","volume":"27","author":"Rienzi","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1093\/humrep\/deh101","article-title":"Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures","volume":"19","author":"Rienzi","year":"2004","journal-title":"Hum. Reprod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/humupd\/dmq029","article-title":"Predictive value of oocyte morphology in human IVF: A systematic review of the literature","volume":"17","author":"Rienzi","year":"2011","journal-title":"Hum. Reprod. Update"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1093\/humupd\/dmr016","article-title":"Gamete competence assessment by polarizing optics in assisted reproduction","volume":"17","author":"Montag","year":"2011","journal-title":"Hum. Reprod. Update"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1093\/humupd\/dmn025","article-title":"ART failure: Oocyte contributions to unsuccessful fertilization","volume":"14","author":"Swain","year":"2008","journal-title":"Hum. Reprod. Update"},{"key":"ref_18","first-page":"244","article-title":"Pilot study of novel noninvasive imaging approach for determination of meiotic status in intact human cumulus-oocyte-complexes","volume":"34","author":"Albertini","year":"2019","journal-title":"Hum. Reprod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.fertnstert.2019.08.070","article-title":"Oocyte meiotic spindle morphology is a predictive marker of blastocyst ploidy-a prospective cohort study","volume":"113","author":"Tilia","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1126\/science.aaa9529","article-title":"Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes","volume":"348","author":"Blayney","year":"2015","journal-title":"Science"},{"key":"ref_21","first-page":"e60058","article-title":"Human Egg Maturity Assessment and Its Clinical Application","volume":"150","author":"Holubcova","year":"2019","journal-title":"J. Vis. Exp."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/S1472-6483(10)61996-7","article-title":"Spindle imaging in human oocytes: The impact of the meiotic cell cycle","volume":"12","author":"Montag","year":"2006","journal-title":"Reprod. Biomed. Online"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.cryobiol.2021.03.001","article-title":"Important parameters affecting quality of vitrified donor oocytes","volume":"100","author":"Tepla","year":"2021","journal-title":"Cryobiology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/rmb2.12100","article-title":"Meiotic spindle size is a strong indicator of human oocyte quality","volume":"17","author":"Tomari","year":"2018","journal-title":"Reprod. Med. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"E341","DOI":"10.1016\/j.fertnstert.2014.07.1155","article-title":"Relationship Between the Meiotic Spindle Size in Human Oocytes and Embryo Developmental Potential After Intracytoplasmic Sperm Injection","volume":"102","author":"Tomari","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1071\/RD18276","article-title":"Vitrifying immature equine oocytes impairs their ability to correctly align the chromosomes on the MII spindle","volume":"31","author":"Ducheyne","year":"2019","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1093\/biolre\/iox145","article-title":"Meiotic spindle formation in mammalian oocytes: Implications for human infertility","volume":"98","author":"Namgoong","year":"2018","journal-title":"Biol. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"755740","DOI":"10.3389\/fcell.2021.755740","article-title":"High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy","volume":"9","author":"Hampl","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1093\/humrep\/deh194","article-title":"DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification","volume":"19","author":"Isachenko","year":"2004","journal-title":"Hum. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.fertnstert.2021.12.012","article-title":"The sixth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen: Ensuring quality and standardization in basic examination of human ejaculates","volume":"117","author":"Bjorndahl","year":"2022","journal-title":"Fertil. Steril."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1093\/humrep\/det312","article-title":"Bisphenol-A and human oocyte maturation in vitro","volume":"28","author":"Machtinger","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1097\/00001703-199906000-00013","article-title":"Culture and transfer of human blastocysts","volume":"11","author":"Gardner","year":"1999","journal-title":"Curr. Opin. Obstet. Gynecol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/S0015-0282(00)00518-5","article-title":"Blastocyst score affects implantation and pregnancy outcome: Towards a single blastocyst transfer","volume":"73","author":"Gardner","year":"2000","journal-title":"Fertil. Steril."},{"key":"ref_34","unstructured":"Jansen, R., and Mortimer, D. (1999). In vitro culture of human blastocyst. Towards Reproductive Certainty: Infertility and Genetics Beyond, Carnforth Parthenon Press."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1017\/S2040174417000368","article-title":"Impact of the IVF laboratory environment on human preimplantation embryo phenotype","volume":"8","author":"Gardner","year":"2017","journal-title":"J. Dev. Orig. Health Dis."},{"key":"ref_36","unstructured":"Stangroom, J. (2022, December 11). Pearson Correlation Coefficient Calculator. Available online: https:\/\/www.socscistatistics.com\/tests\/pearson\/default2.aspx."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1093\/humrep\/deu175","article-title":"Assisted reproductive technology in Europe, 2010: Results generated from European registers by ESHRE","volume":"29","author":"Kupka","year":"2014","journal-title":"Hum. Reprod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/S1472-6483(10)60633-5","article-title":"Optimum number of oocytes for a successful first IVF treatment cycle","volume":"13","author":"Eijkemans","year":"2006","journal-title":"Reprod. Biomed. Online"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1111\/cen.13956","article-title":"Anti-Mullerian hormone variability and its implications for the number of oocytes retrieved following individualized dosing with follitropin delta","volume":"90","author":"Nelson","year":"2019","journal-title":"Clin. Endocrinol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/1\/105\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:45:24Z","timestamp":1760147124000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/1\/105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,21]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["ijms24010105"],"URL":"https:\/\/doi.org\/10.3390\/ijms24010105","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,21]]}}}