{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T14:00:14Z","timestamp":1778594414390,"version":"3.51.4"},"reference-count":31,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["SAF2017-82369-R"],"award-info":[{"award-number":["SAF2017-82369-R"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2020-112870RB-I00"],"award-info":[{"award-number":["PID2020-112870RB-I00"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2020-118054RB-I00"],"award-info":[{"award-number":["PID2020-118054RB-I00"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["2017 SGR 38"],"award-info":[{"award-number":["2017 SGR 38"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["Basic Research 2020"],"award-info":[{"award-number":["Basic Research 2020"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["SAF2017-82369-R"],"award-info":[{"award-number":["SAF2017-82369-R"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2020-112870RB-I00"],"award-info":[{"award-number":["PID2020-112870RB-I00"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2020-118054RB-I00"],"award-info":[{"award-number":["PID2020-118054RB-I00"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["2017 SGR 38"],"award-info":[{"award-number":["2017 SGR 38"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["Basic Research 2020"],"award-info":[{"award-number":["Basic Research 2020"]}]},{"name":"Generalitat de Catalunya","award":["SAF2017-82369-R"],"award-info":[{"award-number":["SAF2017-82369-R"]}]},{"name":"Generalitat de Catalunya","award":["PID2020-112870RB-I00"],"award-info":[{"award-number":["PID2020-112870RB-I00"]}]},{"name":"Generalitat de Catalunya","award":["PID2020-118054RB-I00"],"award-info":[{"award-number":["PID2020-118054RB-I00"]}]},{"name":"Generalitat de Catalunya","award":["2017 SGR 38"],"award-info":[{"award-number":["2017 SGR 38"]}]},{"name":"Generalitat de Catalunya","award":["Basic Research 2020"],"award-info":[{"award-number":["Basic Research 2020"]}]},{"name":"Spanish Atherosclerosis Society","award":["SAF2017-82369-R"],"award-info":[{"award-number":["SAF2017-82369-R"]}]},{"name":"Spanish Atherosclerosis Society","award":["PID2020-112870RB-I00"],"award-info":[{"award-number":["PID2020-112870RB-I00"]}]},{"name":"Spanish Atherosclerosis Society","award":["PID2020-118054RB-I00"],"award-info":[{"award-number":["PID2020-118054RB-I00"]}]},{"name":"Spanish Atherosclerosis Society","award":["2017 SGR 38"],"award-info":[{"award-number":["2017 SGR 38"]}]},{"name":"Spanish Atherosclerosis Society","award":["Basic Research 2020"],"award-info":[{"award-number":["Basic Research 2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We previously demonstrated that treatment with BemA (bempedoic acid), an inhibitor of ATP citrate lyase, significantly reduces fatty liver in a model of liver steatosis (HFHFr\u2014female Sprague-Dawley rat fed a high-fat high-fructose diet). Since the hepatic production of the gasotransmitter H2S is impaired in liver disorders, we were interested in determining if the production of H2S was altered in our HFHFr model and whether the administration of BemA reversed these changes. We used stored liver samples from a previous study to determine the total and enzymatic H2S production, as well as the expression of CBS (cystathionine \u03b2-synthase), CSE (cystathionine \u03b3-lyase), and 3MST (3-mercaptopiruvate sulfurtransferase), and the expression\/activity of FXR (farnesoid X receptor), a transcription factor involved in regulating CSE expression. Our data show that the HFHFr diet reduces the total and enzymatic production of liver H2S, mainly by decreasing the expression of CBS and CSE. Furthermore, BemA treatment restored H2S production, increasing the expression of CBS and CSE, providing evidence for the involvement of FXR transcriptional activity and the mTORC1 (mammalian target of rapamycin1)\/S6K1 (ribosomal protein S6 kinase beta-1)\/PGC1\u03b1 (peroxisome proliferator receptor gamma coactivator1\u03b1) pathway.<\/jats:p>","DOI":"10.3390\/ijms24010473","type":"journal-article","created":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T05:42:48Z","timestamp":1672206168000},"page":"473","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Bempedoic Acid Restores Liver H2S Production in a Female Sprague-Dawley Rat Dietary Model of Non-Alcoholic Fatty Liver"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5018-021X","authenticated-orcid":false,"given":"N\u00faria","family":"Roglans","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Toxicology and Therapeutic Chemistry, School of Pharmacy and Food Science, University of Barcelona, Av. Joan XXIII 27\u201331, 08028 Barcelona, Spain"},{"name":"Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5783-3092","authenticated-orcid":false,"given":"Elena","family":"Fauste","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Montepr\u00edncipe, Boadilla del Monte, 28668 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9714-163X","authenticated-orcid":false,"given":"Roger","family":"Bentanachs","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Therapeutic Chemistry, School of Pharmacy and Food Science, University of Barcelona, Av. Joan XXIII 27\u201331, 08028 Barcelona, Spain"},{"name":"Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M.","family":"Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Therapeutic Chemistry, School of Pharmacy and Food Science, University of Barcelona, Av. Joan XXIII 27\u201331, 08028 Barcelona, Spain"},{"name":"Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madelin","family":"P\u00e9rez-Armas","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Montepr\u00edncipe, Boadilla del Monte, 28668 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2558-9539","authenticated-orcid":false,"given":"Cristina","family":"Donis","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Montepr\u00edncipe, Boadilla del Monte, 28668 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0459-3539","authenticated-orcid":false,"given":"Mar\u00eda I.","family":"Panadero","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Montepr\u00edncipe, Boadilla del Monte, 28668 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5652-8651","authenticated-orcid":false,"given":"Marta","family":"Alegret","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Therapeutic Chemistry, School of Pharmacy and Food Science, University of Barcelona, Av. Joan XXIII 27\u201331, 08028 Barcelona, Spain"},{"name":"Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5107-6185","authenticated-orcid":false,"given":"Paola","family":"Otero","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Montepr\u00edncipe, Boadilla del Monte, 28668 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0364-5958","authenticated-orcid":false,"given":"Carlos","family":"Bocos","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Montepr\u00edncipe, Boadilla del Monte, 28668 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7082-0704","authenticated-orcid":false,"given":"Juan C.","family":"Laguna","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Therapeutic Chemistry, School of Pharmacy and Food Science, University of Barcelona, Av. Joan XXIII 27\u201331, 08028 Barcelona, Spain"},{"name":"Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1002\/med.21913","article-title":"Progress and perspective on hydrogen sulfide donors and their biomedical applications","volume":"42","author":"Song","year":"2022","journal-title":"Med. Res. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Comas, F., and Moreno-Navarrete, J.M. (2021). The Impact of H2S on Obesity-Associated Metabolic Disturbances. 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