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One promising area is genetic therapy that uses small interfering RNA (siRNA). These molecules are capable of blocking mutant proteins in cells, but require specific systems that will deliver RNA to target cells and successfully release them into the cytoplasm. Dendronized and PEGylated silver nanoparticles as potential vectors for proapoptotic siRNA (siMCL-1) were used here. Using the methods of one-dimensional gel electrophoresis, the zeta potential, dynamic light scattering, and circular dichroism, stable siRNA and AgNP complexes were obtained. Data gathered using multicolor flow cytometry showed that AgNPs are able to deliver (up to 90%) siRNAs efficiently to some types of tumor cells, depending on the degree of PEGylation. Analysis of cell death showed that complexes of some AgNP variations with siMCL-1 lead to ~70% cell death in the populations that uptake these complexes due to apoptosis.<\/jats:p>","DOI":"10.3390\/ijms24010840","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T04:08:07Z","timestamp":1672805287000},"page":"840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Silver Nanoparticles Modified by Carbosilane Dendrons and PEG as Delivery Vectors of Small Interfering RNA"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-7076","authenticated-orcid":false,"given":"Viktar","family":"Abashkin","sequence":"first","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"P\u0119dziwiatr-Werbicka","sequence":"additional","affiliation":[{"name":"Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141\/143 Pomorska St., 90-236 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Horodecka","sequence":"additional","affiliation":[{"name":"Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141\/143 Pomorska St., 90-236 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5203-0146","authenticated-orcid":false,"given":"Victoriya","family":"Zhogla","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egor","family":"Ulashchik","sequence":"additional","affiliation":[{"name":"Institute of Physical Organic Chemistry of NASB, 13 Surganova St., 220072 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1775-8101","authenticated-orcid":false,"given":"Vadim","family":"Shmanai","sequence":"additional","affiliation":[{"name":"Institute of Physical Organic Chemistry of NASB, 13 Surganova St., 220072 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1414-4465","authenticated-orcid":false,"given":"Dzmitry","family":"Shcharbin","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4676-3743","authenticated-orcid":false,"given":"Maria","family":"Bryszewska","sequence":"additional","affiliation":[{"name":"Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141\/143 Pomorska St., 90-236 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. 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