{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:53:22Z","timestamp":1787756002897,"version":"build-2784847793"},"reference-count":19,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T00:00:00Z","timestamp":1672790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["16K09946"],"award-info":[{"award-number":["16K09946"]}]},{"name":"JSPS KAKENHI","award":["19K08939"],"award-info":[{"award-number":["19K08939"]}]},{"name":"Nihon University Multidisciplinary Research Grant for 2016","award":["16K09946"],"award-info":[{"award-number":["16K09946"]}]},{"name":"Nihon University Multidisciplinary Research Grant for 2016","award":["19K08939"],"award-info":[{"award-number":["19K08939"]}]},{"name":"Nihon University Research Grant for 2022","award":["16K09946"],"award-info":[{"award-number":["16K09946"]}]},{"name":"Nihon University Research Grant for 2022","award":["19K08939"],"award-info":[{"award-number":["19K08939"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mycobacterium tuberculosis (Mtb) infection remains a major health problem worldwide. Although the Bacillus Calmette-Gu\u00e9rin (BCG) vaccine is the most widely used vaccination for preventing tuberculosis (TB), its efficacy is limited. We previously developed a new recombinant BCG (rBCG)-based vaccine encoding the Ag85B protein of M. kansasii (Mkan85B), termed rBCG-Mkan85B, and its administration is followed by boosting with plasmid DNA expressing the Ag85B gene (DNA-Mkan85B). Previously, we identified MHC-I (H2-Kd)-restricted epitopes that highly cross-react with those of Mtb in BALB\/c (H2d) and CB6F1 (H2b\/d) mice. We also reported that the rBCG-Mkan85B\/DNA-Mkan85B prime\u2013boost vaccination protocol protected CB6F1 mice against M. kansasii infection. In this study, to investigate the protective effect of our novel rBCG against Mtb infection, CB6F1 mice were either left unimmunized or immunized with the BCG, rBCG-Mkan85B, or rBCG-Mkan85B\/DNA-Mkan85B vaccine for 10 weeks prior to inhalation exposure to the virulent Mtb Erdman strain for another 6 weeks. Compared with the BCG and rBCG-Mkan85B vaccinations, the rBCG-Mkan85B\/DNA-Mkan85B prime\u2013boost vaccination protocol significantly reduced the numbers of pulmonary colony-forming units (CFUs). Moreover, the rBCG-Mkan85B\/DNA-Mkan85B prime\u2013boost vaccination induced antigen-specific polyfunctional CD4+ and CD8+ T cells. These results suggest that CD8+ T-cell immunity to immunodominant epitopes of Mtb is enhanced by rBCG vector-based immunization. Thus, rBCG vector-based vaccinations may overcome the limited ability of the current BCG vaccine to elicit TB immunity.<\/jats:p>","DOI":"10.3390\/ijms24020966","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T03:12:15Z","timestamp":1672888335000},"page":"966","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Induction of Antigen 85B-Specific CD8+ T Cells by Recombinant BCG Protects against Mycobacterial Infection in Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9417-0315","authenticated-orcid":false,"given":"Shihoko","family":"Komine-Aizawa","sequence":"first","affiliation":[{"name":"Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Tokyo 173-8610, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Japan BCG Laboratory, 3-1-5 Matsuyama, Tokyo 204-0022, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Kawano","sequence":"additional","affiliation":[{"name":"Japan BCG Laboratory, 3-1-5 Matsuyama, Tokyo 204-0022, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-1443","authenticated-orcid":false,"given":"Satoshi","family":"Hayakawa","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Tokyo 173-8610, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Japan BCG Laboratory, 3-1-5 Matsuyama, Tokyo 204-0022, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Honda","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Tokyo 173-8610, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1002\/eji.201847988","article-title":"MHC-restricted Ag85B-specific CD8(+) T cells are enhanced by recombinant BCG prime and DNA boost immunization in mice","volume":"49","author":"Jiang","year":"2019","journal-title":"Eur. 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