{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:47:09Z","timestamp":1786949229147,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016132","name":"Walloon Government","doi-asserted-by":"publisher","award":["1610151"],"award-info":[{"award-number":["1610151"]}],"id":[{"id":"10.13039\/100016132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016132","name":"Walloon Government","doi-asserted-by":"publisher","award":["0325777171"],"award-info":[{"award-number":["0325777171"]}],"id":[{"id":"10.13039\/100016132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005627","name":"University of Liege","doi-asserted-by":"publisher","award":["1610151"],"award-info":[{"award-number":["1610151"]}],"id":[{"id":"10.13039\/501100005627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005627","name":"University of Liege","doi-asserted-by":"publisher","award":["0325777171"],"award-info":[{"award-number":["0325777171"]}],"id":[{"id":"10.13039\/501100005627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We investigated the antioxidant potential of equine mesenchymal stem cells derived from muscle microbiopsies (mdMSCs), loaded by a water-soluble curcumin lysinate incorporated into hydroxypropyl-\u03b2-cyclodextrin (NDS27). The cell loading was rapid and dependent on NDS27 dosage (14, 7, 3.5 and 1 \u00b5M). The immunomodulatory capacity of loaded mdMSCs was evaluated by ROS production, on active and total myeloperoxidase (MPO) degranulation and neutrophil extracellular trap (NET) formation after neutrophil stimulation. The intracellular protection of loaded cells was tested by an oxidative stress induced by cumene hydroperoxide. Results showed that 10 min of mdMSC loading with NDS27 did not affect their viability while reducing their metabolism. NDS27 loaded cells in presence of 14, 7 \u00b5M NDS27 inhibited more intensively the ROS production, the activity of the MPO released and bound to the NET after neutrophil stimulation. Furthermore, loaded cells powerfully inhibited intracellular ROS production induced by cumene as compared to control cells or cyclodextrin-loaded cells. Our results showed that the loading of mdMSCs with NDS27 significantly improved their antioxidant potential against the oxidative burst of neutrophil and protected them against intracellular ROS production. The improved antioxidant protective capacity of loaded mdMSCs could be applied to target inflammatory foci involving neutrophils.<\/jats:p>","DOI":"10.3390\/ijms24021030","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T05:29:48Z","timestamp":1672896588000},"page":"1030","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Equine Muscle Derived Mesenchymal Stem Cells Loaded with Water-Soluble Curcumin: Modulation of Neutrophil Activation and Enhanced Protection against Intracellular Oxidative Attack"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4863-805X","authenticated-orcid":false,"given":"Thierry","family":"Franck","sequence":"first","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"},{"name":"Research Unit FARAH, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine","family":"Ceusters","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"},{"name":"Research Unit FARAH, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Graide","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Niesten","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Duysens","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ange Mouithys","family":"Mickalad","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Serteyn","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"},{"name":"Research Unit FARAH, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e12712","DOI":"10.1111\/cpr.12712","article-title":"Immune Modulation by Mesenchymal Stem Cells","volume":"53","author":"Jiang","year":"2020","journal-title":"Cell Prolif."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1167\/iovs.17-23067","article-title":"Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation","volume":"59","author":"Mittal","year":"2018","journal-title":"Invest. Ophthal. Mol. Vis. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/s41598-017-00803-7","article-title":"From Skeletal Muscle to Stem Cells: An Innovative and Minimally-Invasive Process for Multiple Species","volume":"7","author":"Ceusters","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Franck, T., Ceusters, J., Graide, H., Mouithys-Mickalad, A., and Serteyn, D. (2021). Muscle Derived Mesenchymal Stem Cells Inhibit the Activity of the Free and the Neutrophil Extracellular Trap (NET)-Bond Myeloperoxidase. Cells, 10.","DOI":"10.3390\/cells10123486"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"53","DOI":"10.3892\/br.2022.1536","article-title":"Role of Myeloperoxidase in Inflammation and Atherosclerosis (Review)","volume":"16","author":"Frangie","year":"2022","journal-title":"Biomed. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Andr\u00e9s, C.M.C., P\u00e9rez de la Lastra, J.M., Juan, C.A., Plou, F.J., and P\u00e9rez-Lebe\u00f1a, E. (2022). Hypochlorous Acid Chemistry in Mammalian Cells-Influence on Infection and Role in Various Pathologies. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810735"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1189\/jlb.0711387","article-title":"Myeloperoxidase Associated with Neutrophil Extracellular Traps Is Active and Mediates Bacterial Killing in the Presence of Hydrogen Peroxide","volume":"91","author":"Parker","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.freeradbiomed.2021.07.007","article-title":"Role of Myeloperoxidase and Oxidant Formation in the Extracellular Environment in Inflammation-Induced Tissue Damage","volume":"172","author":"Hawkins","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"102109","DOI":"10.1016\/j.redox.2021.102109","article-title":"The Many Roles of Myeloperoxidase: From Inflammation and Immunity to Biomarkers, Drug Metabolism and Drug Discovery","volume":"46","author":"Siraki","year":"2021","journal-title":"Redox. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s13287-019-1224-y","article-title":"Priming Approaches to Improve the Efficacy of Mesenchymal Stromal Cell-Based Therapies","volume":"10","author":"Mizukami","year":"2019","journal-title":"Stem. Cell Res. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1615\/CritRevTherDrugCarrierSyst.2021033916","article-title":"Mesenchymal Stem Cells: From Regeneration to Drug Delivery Systems","volume":"38","author":"Shokat","year":"2021","journal-title":"Crit. Rev. Ther. Drug Carrier Syst."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1080\/26895293.2021.1967202","article-title":"Stem Cell and Its Derivatives as Drug Delivery Vehicles: An Effective New Strategy of Drug Delivery System","volume":"14","author":"Fu","year":"2021","journal-title":"All Life"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"347","DOI":"10.2174\/1389450119666180731092453","article-title":"Potential Non-Neoplastic Applications for Polyphenols in Stem Cell Utilization","volume":"20","author":"Cherniack","year":"2019","journal-title":"Curr. Drug Targets"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jphs.2016.10.005","article-title":"Curcumin Protects Human Adipose-Derived Mesenchymal Stem Cells against Oxidative Stress-Induced Inhibition of Osteogenesis","volume":"132","author":"Wang","year":"2016","journal-title":"J. Pharmacol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.freeradbiomed.2020.07.023","article-title":"Hypoxic Preconditioning Combined with Curcumin Promotes Cell Survival and Mitochondrial Quality of Bone Marrow Mesenchymal Stem Cells, and Accelerates Cutaneous Wound Healing via PGC-1\u03b1\/SIRT3\/HIF-1\u03b1 Signaling","volume":"159","author":"Wang","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-0-387-46401-5_1","article-title":"Curcumin: The Indian Solid Gold","volume":"595","author":"Aggarwal","year":"2007","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1111\/bph.13621","article-title":"Curcumin, the Golden Nutraceutical: Multitargeting for Multiple Chronic Diseases","volume":"174","author":"Kunnumakkara","year":"2017","journal-title":"Br. J. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1016\/j.lfs.2005.12.007","article-title":"Multiple Biological Activities of Curcumin: A Short Review","volume":"78","author":"Maheshwari","year":"2006","journal-title":"Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Barzegar, A., and Moosavi-Movahedi, A.A. (2011). Intracellular ROS Protection Efficiency and Free Radical-Scavenging Activity of Curcumin. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026012"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"577","DOI":"10.33549\/physiolres.931086","article-title":"Inhibitory Effect of Curcuminoids and Tetrahydrocurcuminoids on Equine Activated Neutrophils and Myeloperoxidase Activity","volume":"57","author":"Franck","year":"2008","journal-title":"Physiol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"638153","DOI":"10.1155\/2015\/638153","article-title":"Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis","volume":"2015","author":"Liu","year":"2015","journal-title":"Stem Cells Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jphotochemrev.2009.05.001","article-title":"Photophysics, Photochemistry and Photobiology of Curcumin: Studies from Organic Solutions, Bio-Mimetics and Living Cells","volume":"10","author":"Priyadarsini","year":"2009","journal-title":"J. Photochem. Photobiol. C Photochem. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.fob.2014.11.004","article-title":"NDS27 Combines the Effect of Curcumin Lysinate and Hydroxypropyl-\u03b2-Cyclodextrin to Inhibit Equine PKC\u03b4 and NADPH Oxidase Involved in the Oxidative Burst of Neutrophils","volume":"4","author":"Derochette","year":"2014","journal-title":"FEBS Open Bio"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.cbi.2018.10.008","article-title":"The Soluble Curcumin Derivative NDS27 Inhibits Superoxide Anion Production by Neutrophils and Acts as Substrate and Reversible Inhibitor of Myeloperoxidase","volume":"297","author":"Franck","year":"2019","journal-title":"Chem. Biol. Interact."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/j.talanta.2015.08.007","article-title":"EquiNox2: A New Method to Measure NADPH Oxidase Activity and to Study Effect of Inhibitors and Their Interactions with the Enzyme","volume":"144","author":"Derochette","year":"2015","journal-title":"Talanta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1136\/vr.d2618","article-title":"Inhalation with NDS27 Attenuates Pulmonary Neutrophilic Inflammation in Recurrent Airway Obstruction","volume":"169","author":"Sandersen","year":"2011","journal-title":"Vet. Rec."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s40248-015-0010-7","article-title":"Effect of Inhaled Hydrosoluble Curcumin on Inflammatory Markers in Broncho-Alveolar Lavage Fluid of Horses with LPS-Induced Lung Neutrophilia","volume":"10","author":"Sandersen","year":"2015","journal-title":"Multidiscip. Respir. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4877","DOI":"10.1111\/jcmm.16403","article-title":"Priming of Mesenchymal Stem Cells with a Hydrosoluble Form of Curcumin Allows Keeping Their Mesenchymal Properties for Cell-Based Therapy Development","volume":"25","author":"Colin","year":"2021","journal-title":"J. Cell Mol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1177\/104063870501700502","article-title":"Development of an Enzyme-Linked Immunosorbent Assay for Specific Equine Neutrophil Myeloperoxidase Measurement in Blood","volume":"17","author":"Franck","year":"2005","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_30","unstructured":"Neven, P., Serteyn, D., Delarge, J., Kiss, R., Mathieu, V., Cataldo, D., and Rocks, N. (2009). Water Soluble Curcumin Compositions for Use in Anti-Cancer and Anti-Inflammatory Therapy. (Patent: WO\/2009\/144220)."},{"key":"ref_31","first-page":"A.3B.1","article-title":"Trypan Blue Exclusion Test of Cell Viability","volume":"21","author":"Strober","year":"2001","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0034-5288(18)30727-6","article-title":"Rapid, Single-Step Isolation of Equine Neutrophils on a Discontinuous Percoll Density Gradient","volume":"42","author":"Pycock","year":"1987","journal-title":"Res. Vet. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1007\/s12026-017-8905-3","article-title":"Validation of an Enzyme-Linked Immunosorbent Assay for the Quantification of Citrullinated Histone H3 as a Marker for Neutrophil Extracellular Traps in Human Plasma","volume":"65","author":"Daleskog","year":"2017","journal-title":"Immunol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"23105","DOI":"10.1074\/jbc.271.38.23105","article-title":"Effect of Oxidative Stress, Produced by Cumene Hydroperoxide, on the Various Steps of Protein Synthesis. Modifications of Elongation Factor-2","volume":"271","author":"Ayala","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/s13287-022-02822-2","article-title":"Potential Pre-Activation Strategies for Improving Therapeutic Efficacy of Mesenchymal Stem Cells: Current Status and Future Prospects","volume":"13","author":"Li","year":"2022","journal-title":"Stem Cell Res. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1177\/2320206820949741","article-title":"Comparison of Effects of Curcumin and Nano-Curcumin on the Survival of Human-Derived Mesenchymal Stem Cells: An Experimental Study","volume":"11","author":"Hameeda","year":"2020","journal-title":"J. Adv. Oral Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.cbi.2019.01.010","article-title":"Curcumin Analogues Attenuate A\u03b2 25-35 -Induced Oxidative Stress in PC12 Cells via Keap1\/Nrf2\/HO-1 Signaling Pathways","volume":"305","author":"Xu","year":"2019","journal-title":"Chem. Biol. Interact."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s13287-018-0768-6","article-title":"Curcumin-Mediated Bone Marrow Mesenchymal Stem Cell Sheets Create a Favorable Immune Microenvironment for Adult Full-Thickness Cutaneous Wound Healing","volume":"9","author":"Yang","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"tkab021","DOI":"10.1093\/burnst\/tkab021","article-title":"Curcumin Preconditioning Enhances the Efficacy of Adipose-Derived Mesenchymal Stem Cells to Accelerate Healing of Burn Wounds","volume":"9","author":"Azam","year":"2021","journal-title":"Burns Trauma"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.ijpharm.2017.02.005","article-title":"Stem Cell-Extracellular Vesicles as Drug Delivery Systems: New Frontiers for Silk\/Curcumin Nanoparticles","volume":"520","author":"Perteghella","year":"2017","journal-title":"Int. J. Pharm."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Gorabi, A.M., Kiaie, N., Hajighasemi, S., Jamialahmadi, T., Majeed, M., and Sahebkar, A. (2019). The Effect of Curcumin on the Differentiation of Mesenchymal Stem Cells into Mesodermal Lineage. Molecules, 24.","DOI":"10.3390\/molecules24224029"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.colsurfb.2014.11.034","article-title":"Mesenchymal Stromal Cells Loading Curcumin-INVITE-Micelles: A Drug Delivery System for Neurodegenerative Diseases","volume":"125","author":"Tripodo","year":"2015","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e000935","DOI":"10.1136\/bmjsem-2020-000935","article-title":"Therapeutic Effects of Turmeric or Curcumin Extract on Pain and Function for Individuals with Knee Osteoarthritis: A Systematic Review","volume":"7","author":"Paultre","year":"2021","journal-title":"BMJ. Open Sport Exerc. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4503","DOI":"10.2147\/DDDT.S327378","article-title":"Anti-Inflammatory Effects of Curcumin in the Inflammatory Diseases: Status, Limitations and Countermeasures","volume":"15","author":"Peng","year":"2021","journal-title":"Drug Des. Devel Ther."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Zieli\u0144ska, A., Alves, H., Marques, V., Durazzo, A., Lucarini, M., Alves, T.F., Morsink, M., Willemen, N., Eder, P., and Chaud, M.V. (2020). Properties, Extraction Methods, and Delivery Systems for Curcumin as a Natural Source of Beneficial Health Effects. Medicina, 56.","DOI":"10.3390\/medicina56070336"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"15017","DOI":"10.1038\/cddiscovery.2015.17","article-title":"Curcumin Induces Crosstalk between Autophagy and Apoptosis Mediated by Calcium Release from the Endoplasmic Reticulum, Lysosomal Destabilization and Mitochondrial Events","volume":"1","author":"Moustapha","year":"2015","journal-title":"Cell Death Discov."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sala de Oyanguren, F.J., Rainey, N.E., Moustapha, A., Saric, A., Sureau, F., O\u2019Connor, J.E., and Petit, P.X. (2020). Highlighting Curcumin-Induced Crosstalk between Autophagy and Apoptosis as Supported by Its Specific Subcellular Localization. Cells, 9.","DOI":"10.3390\/cells9020361"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1039\/D1FO03234H","article-title":"The Inhibition of Interaction with Serum Albumin Enhances the Physiological Activity of Curcumin by Increasing Its Cellular Uptake","volume":"13","author":"Itaya","year":"2022","journal-title":"Food Funct."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1089\/adt.2020.1027","article-title":"A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and \u03b2-Mercaptoethanol","volume":"19","author":"Wang","year":"2021","journal-title":"Assay Drug Dev. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1002\/ardp.201400266","article-title":"Mitochondria as a Target in the Therapeutic Properties of Curcumin","volume":"347","author":"Trujillo","year":"2014","journal-title":"Arch. Pharm."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/biof.1566","article-title":"Effects of Curcumin on Mitochondria in Neurodegenerative Diseases","volume":"46","author":"Bagheri","year":"2020","journal-title":"BioFactors"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"118091","DOI":"10.1016\/j.lfs.2020.118091","article-title":"Curcumin Preconditioned Human Adipose Derived Stem Cells Co-Transplanted with Platelet Rich Plasma Improve Wound Healing in Diabetic Rats","volume":"257","author":"Ghufran","year":"2020","journal-title":"Life Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s12951-022-01339-3","article-title":"Curcumin PriMed ADMSCs Derived Small Extracellular Vesicle Exert Enhanced Protective Effects on Osteoarthritis by Inhibiting Oxidative Stress and Chondrocyte Apoptosis","volume":"20","author":"Xu","year":"2022","journal-title":"J. Nanobiotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1476-5381.2011.01590.x","article-title":"Amelioration of Renal Ischaemia-Reperfusion Injury by Liposomal Delivery of Curcumin to Renal Tubular Epithelial and Antigen-Presenting Cells","volume":"166","author":"Rogers","year":"2012","journal-title":"Br. J. Pharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"360438","DOI":"10.1155\/2014\/360438","article-title":"Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal","volume":"2014","author":"Ayala","year":"2014","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.neuroscience.2014.12.055","article-title":"Oxidative Stress Induced by Cumene Hydroperoxide Evokes Changes in Neuronal Excitability of Rat Motor Cortex Neurons","volume":"289","author":"Carrascal","year":"2015","journal-title":"Neuroscience"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s10863-006-9011-8","article-title":"Testing the Vicious Cycle Theory of Mitochondrial ROS Production: Effects of H2O2 and Cumene Hydroperoxide Treatment on Heart Mitochondria","volume":"38","author":"Sanz","year":"2006","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1016\/j.freeradbiomed.2013.09.017","article-title":"On the Use of L-012, a Luminol-Based Chemiluminescent Probe, for Detecting Superoxide and Identifying Inhibitors of NADPH Oxidase: A Reevaluation","volume":"65","author":"Zielonka","year":"2013","journal-title":"Free Radic. Biol. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"101968","DOI":"10.1016\/j.tice.2022.101968","article-title":"Tumoricidal Effects of UnpriMed and Curcumin-PriMed Adipose-Derived Stem Cells on Human Hepatoma HepG2 Cells under Oxidative Conditions","volume":"79","author":"Ghufran","year":"2022","journal-title":"Tissue Cell"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/2\/1030\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:59:52Z","timestamp":1760119192000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/2\/1030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,5]]},"references-count":59,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["ijms24021030"],"URL":"https:\/\/doi.org\/10.3390\/ijms24021030","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,5]]}}}