{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:09:55Z","timestamp":1790633395530,"version":"4.1.0"},"reference-count":64,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation programme under Grant agreement","award":["825712"],"award-info":[{"award-number":["825712"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation programme under Grant agreement","award":["PROMETEO\/2020\/006"],"award-info":[{"award-number":["PROMETEO\/2020\/006"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation programme under Grant agreement","award":["PID2020-113112RB-I00"],"award-info":[{"award-number":["PID2020-113112RB-I00"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation programme under Grant agreement","award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}]},{"name":"Generalitat Valenciana","award":["825712"],"award-info":[{"award-number":["825712"]}]},{"name":"Generalitat Valenciana","award":["PROMETEO\/2020\/006"],"award-info":[{"award-number":["PROMETEO\/2020\/006"]}]},{"name":"Generalitat Valenciana","award":["PID2020-113112RB-I00"],"award-info":[{"award-number":["PID2020-113112RB-I00"]}]},{"name":"Generalitat Valenciana","award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}]},{"name":"CIBERDEM","award":["825712"],"award-info":[{"award-number":["825712"]}]},{"name":"CIBERDEM","award":["PROMETEO\/2020\/006"],"award-info":[{"award-number":["PROMETEO\/2020\/006"]}]},{"name":"CIBERDEM","award":["PID2020-113112RB-I00"],"award-info":[{"award-number":["PID2020-113112RB-I00"]}]},{"name":"CIBERDEM","award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Humans are constantly exposed to many environmental pollutants, some of which have been largely acknowledged as key factors in the development of metabolic disorders such as diabetes and obesity. These chemicals have been classified as endocrine-disrupting chemicals (EDCs) and, more recently, since they can interfere with metabolic functions, they have been renamed as metabolism-disrupting chemicals (MDCs). MDCs are present in many consumer products, including food packaging, personal care products, plastic bottles and containers, and detergents. The scientific literature has ever-increasingly focused on insulin-releasing pancreatic \u03b2-cells as one of the main targets for MDCs. Evidence highlights that these substances may disrupt glucose homeostasis by altering pancreatic \u03b2-cell physiology. However, their potential impact on glucagon-secreting pancreatic \u03b1-cells remains poorly known despite the essential role that this cellular type plays in controlling glucose metabolism. In the present study, we have selected seven paradigmatic MDCs representing major toxic classes, including bisphenols, phthalates, perfluorinated compounds, metals, and pesticides. By using an in vitro cell-based model, the pancreatic \u03b1-cell line \u03b1TC1-9, we have explored the effects of these compounds on pancreatic \u03b1-cell viability, gene expression, and secretion. We found that cell viability was moderately affected after bisphenol-A (BPA), bisphenol-F (BPF), and perfluorooctanesulfonic acid (PFOS) exposure, although cytotoxicity was relatively low. In addition, all bisphenols, as well as di(2-ethylhexyl) phthalate (DEHP) and cadmium chloride (CdCl2), promoted a marked decreased on glucagon secretion, together with changes in the expression of glucagon and\/or transcription factors involved in cell function and identity, such as Foxo1 and Arx. Overall, our results indicated that most of the selected chemicals studied caused functional alterations in pancreatic \u03b1-cells. Moreover, we revealed, for the first time, their direct effects on key molecular aspects of pancreatic \u03b1-cell biology.<\/jats:p>","DOI":"10.3390\/ijms24021044","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T05:29:48Z","timestamp":1672896588000},"page":"1044","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Exploring the Effects of Metabolism-Disrupting Chemicals on Pancreatic \u03b1-Cell Viability, Gene Expression and Function: A Screening Testing Approach"],"prefix":"10.3390","volume":"24","author":[{"given":"Ruba","family":"Al-Abdulla","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE), Universidad Miguel Hern\u00e1ndez, 03202 Elche, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilda","family":"Ferrero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE), Universidad Miguel Hern\u00e1ndez, 03202 Elche, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6548-5251","authenticated-orcid":false,"given":"Tal\u00eda","family":"Boronat-Belda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE), Universidad Miguel Hern\u00e1ndez, 03202 Elche, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergi","family":"Soriano","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE), Universidad Miguel Hern\u00e1ndez, 03202 Elche, Spain"},{"name":"Departamento de Fisiolog\u00eda, Gen\u00e9tica y Microbiolog\u00eda, Universidad de Alicante, 03690 Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Quesada","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE), Universidad Miguel Hern\u00e1ndez, 03202 Elche, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1065-5388","authenticated-orcid":false,"given":"Paloma","family":"Alonso-Magdalena","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Biotecnolog\u00eda Sanitaria de Elche (IDiBE), Universidad Miguel Hern\u00e1ndez, 03202 Elche, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1469-7793.1999.0085z.x","article-title":"Homologous and heterologous asynchronicity between identified alpha-, beta- and delta-cells within intact islets of Langerhans in the mouse","volume":"517","author":"Nadal","year":"1999","journal-title":"J. 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