{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:44:32Z","timestamp":1787244272130,"version":"build-2736575974"},"reference-count":45,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Royal Society Marsden grant"},{"name":"Lottery Health Research grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The \u0394133p53\u03b2 isoform is increased in many primary tumors and has many tumor-promoting properties that contribute to increased proliferation, migration and inflammation. Here we investigated whether \u0394133p53\u03b2 contributed to some of the most aggressive tumors that had metastasized to the brain. \u0394133p53\u03b2 mRNA expression was measured in lung, breast, melanoma, colorectal metastases and, where available, the matched primary tumor. The presence of \u0394133p53\u03b2 expression was associated with the time for the primary tumor to metastasize and overall survival once the tumor was detected in the brain. \u0394133p53\u03b2 was present in over 50% of lung, breast, melanoma and colorectal metastases to the brain. It was also increased in the brain metastases compared with the matched primary tumor. Brain metastases with \u0394133p53\u03b2 expressed were associated with a reduced time for the primary tumor to metastasize to the brain compared with tumors with no \u0394133p53\u03b2 expression. In-vitro-based analyses in \u0394133p53\u03b2-expressing cells showed increased cancer-promoting proteins on the cell surface and increased downstream p-AKT and p-MAPK signaling. \u0394133p53\u03b2-expressing cells also invaded more readily across a mock blood\u2013brain barrier. Together these data suggested that \u0394133p53\u03b2 contributes to brain metastases by making cells more likely to invade the brain.<\/jats:p>","DOI":"10.3390\/ijms24021267","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T02:09:39Z","timestamp":1673230179000},"page":"1267","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Increased Expression of the \u0394133p53\u03b2 Isoform Enhances Brain Metastasis"],"prefix":"10.3390","volume":"24","author":[{"given":"Alexandra N. Boix De","family":"Jesus","sequence":"first","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Taha","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Southern District Health Board, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulomi M.","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"},{"name":"The Westmead Institute for Medical Research, Sydney, NSW 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1777-1245","authenticated-orcid":false,"given":"Sunali","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"},{"name":"Maurice Wilkins Centre for Biodiscovery, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley","family":"Reily-Bell","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasini Polwatta","family":"Lekamlage","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Machado","family":"Saraiva","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahmeed","family":"Tahmeedzaman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fouzia","family":"Ziad","sequence":"additional","affiliation":[{"name":"Department of Pathology, Waikato District Health Board, Hamilton 3204, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziad","family":"Thotathil","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Waikato District Health Board, Hamilton 3204, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Y. C.","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Waikato District Health Board, Hamilton 3204, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1464-2197","authenticated-orcid":false,"given":"Janice","family":"Royds","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony","family":"Braithwaite","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"},{"name":"Maurice Wilkins Centre for Biodiscovery, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelyn","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4031-0604","authenticated-orcid":false,"given":"Tania L.","family":"Slatter","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand"},{"name":"Maurice Wilkins Centre for Biodiscovery, University of Otago, Dunedin 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e14734","DOI":"10.7554\/eLife.14734","article-title":"TP53 drives invasion through expression of its Delta133p53beta variant","volume":"5","author":"Gadea","year":"2016","journal-title":"Elife"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/s41419-019-1861-1","article-title":"The Delta133p53beta isoform promotes an immunosuppressive environment leading to aggressive prostate cancer","volume":"10","author":"Kazantseva","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/path.5111","article-title":"Elevation of the TP53 isoform Delta133p53beta in glioblastomas: An alternative to mutant p53 in promoting tumour development","volume":"246","author":"Kazantseva","year":"2018","journal-title":"J. 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