{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:16Z","timestamp":1787028496097,"version":"build-2736575974"},"reference-count":45,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009882","name":"Lombardy region","doi-asserted-by":"publisher","award":["2014IT16RFOP012-POR FESR 2014-2020-Project ID226149"],"award-info":[{"award-number":["2014IT16RFOP012-POR FESR 2014-2020-Project ID226149"]}],"id":[{"id":"10.13039\/501100009882","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The urogenital microbiota is dominated by Lactobacillus that, together with Bifidobacterium, creates a physiological barrier counteracting pathogen infections. The aim of this study was to evaluate the efficacy of a multi-strain probiotic formulation (Lactiplantibacillus plantarum PBS067, Lacticaseibacillus rhamnosus LRH020, and Bifidobacterium animalis subsp. lactis BL050) to inhibit adhesion and growth of urogenital pathogens. The antimicrobial and antiadhesive properties of the probiotic strains and their mixture were evaluated on human vaginal epithelium infected with Candida glabrata, Neisseria gonorrheae, Trichomonas vaginalis, and Escherichia coli-infected human bladder epithelium. The epithelial tissue permeability and integrity were assessed by transepithelial\/transendothelial electrical resistance (TEER). Co-aggregation between probiotics and vaginal pathogens was also investigated to elucidate a possible mechanism of action. The multi-strain formulation showed a full inhibition of T. vaginalis, and a reduction in C. glabrata and N. gonorrheae growth. A relevant antimicrobial activity was observed for each single strain against E. coli. TEER results demonstrated that none of the strains have negatively impaired the integrity of the 3D tissues. All the probiotics and their mixture were able to form aggregates with the tested pathogens. The study demonstrated that the three strains and their mixture are effective to prevent urogenital infections.<\/jats:p>","DOI":"10.3390\/ijms24021323","type":"journal-article","created":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T01:57:48Z","timestamp":1673315868000},"page":"1323","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens"],"prefix":"10.3390","volume":"24","author":[{"given":"Patrizia","family":"Malfa","sequence":"first","affiliation":[{"name":"SynBalance srl, 21040 Origgio, VA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Brambilla","sequence":"additional","affiliation":[{"name":"VitroScreen srl, 20149 Milan, MI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Giardina","sequence":"additional","affiliation":[{"name":"Complife Italia srl, 20024 Garbagnate Milanese, MI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Masciarelli","sequence":"additional","affiliation":[{"name":"Complife Italia srl, 20024 Garbagnate Milanese, MI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8090-7718","authenticated-orcid":false,"given":"Diletta Francesca","family":"Squarzanti","sequence":"additional","affiliation":[{"name":"SynBalance srl, 21040 Origgio, VA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9911-7094","authenticated-orcid":false,"given":"Federica","family":"Carlomagno","sequence":"additional","affiliation":[{"name":"Roelmi HPC srl, 21040 Origgio, VA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Meloni","sequence":"additional","affiliation":[{"name":"VitroScreen srl, 20149 Milan, MI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1097\/MAJ.0b013e31823ea228","article-title":"The microbiota of the vagina and its influence on women\u2019s health and disease","volume":"343","author":"Martin","year":"2012","journal-title":"Am. 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