{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T01:55:09Z","timestamp":1787968509566,"version":"build-2784847793"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Pecs Medical School Research","award":["PTE-\u00c1OK KA 2021-38"],"award-info":[{"award-number":["PTE-\u00c1OK KA 2021-38"]}]},{"name":"University of Pecs Medical School Research","award":["KA 2019-48"],"award-info":[{"award-number":["KA 2019-48"]}]},{"name":"University of Pecs Medical School Research","award":["\u00daNKP-20-5-PTE-1727"],"award-info":[{"award-number":["\u00daNKP-20-5-PTE-1727"]}]},{"name":"Hungarian Academy of Sciences","award":["PTE-\u00c1OK KA 2021-38"],"award-info":[{"award-number":["PTE-\u00c1OK KA 2021-38"]}]},{"name":"Hungarian Academy of Sciences","award":["KA 2019-48"],"award-info":[{"award-number":["KA 2019-48"]}]},{"name":"Hungarian Academy of Sciences","award":["\u00daNKP-20-5-PTE-1727"],"award-info":[{"award-number":["\u00daNKP-20-5-PTE-1727"]}]},{"name":"National Research, Development and Innovation Fund","award":["PTE-\u00c1OK KA 2021-38"],"award-info":[{"award-number":["PTE-\u00c1OK KA 2021-38"]}]},{"name":"National Research, Development and Innovation Fund","award":["KA 2019-48"],"award-info":[{"award-number":["KA 2019-48"]}]},{"name":"National Research, Development and Innovation Fund","award":["\u00daNKP-20-5-PTE-1727"],"award-info":[{"award-number":["\u00daNKP-20-5-PTE-1727"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although many studies have investigated the clinical aspect of early-onset preeclampsia, our knowledge about the immunological consequences of improper placenta development is scarce. The maternal immunotolerance against the fetus is greatly influenced by the Th1 predominance developed by the mother\u2019s immune system. Thirty-two early-onset preeclamptic and fifty-one healthy pregnant women with appropriately matched gestational age were involved in our study. Mononuclear cells were separated from peripheral venous blood and the frequency of CD8\u207a, CD4\u207a, double positive (DP), and double negative (DN) NKT cell subpopulations was determined using multicolor flow cytometry. Following the characterization, the expression levels of different immune checkpoint receptors and ligands were also defined. Soluble CD226 levels were quantified by ELISA. Novel and significant differences were revealed among the ratios of the investigated NKT subsets and in the expression patterns of PD-1, LAG-3, TIGIT and CD226 receptors. Further differences were determined in the expression of CD112, PD-1, LAG-3 and CD226 MFI values between the early-onset preeclamptic and the healthy pregnant groups. Our results suggest that the investigated NKT subpopulations act differently in the altered immune condition characteristic of early-onset preeclampsia and indicate that the different subsets may contribute to the compensation or maintenance of Th1 predominance.<\/jats:p>","DOI":"10.3390\/ijms24021390","type":"journal-article","created":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T02:06:03Z","timestamp":1673402763000},"page":"1390","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["CD8 and CD4 Positive NKT Subpopulations and Immune-Checkpoint Pathways in Early-Onset Preeclampsia and Healthy Pregnancy"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9913-2024","authenticated-orcid":false,"given":"Matyas","family":"Meggyes","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical School, University of Pecs, 12 Szigeti Street, 7624 Pecs, Hungary"},{"name":"Janos Szentagothai Research Centre, 20 Ifjusag Street, 7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7776-5226","authenticated-orcid":false,"given":"Timoteus","family":"Feik","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical School, University of Pecs, 12 Szigeti Street, 7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7742-4459","authenticated-orcid":false,"given":"David U.","family":"Nagy","sequence":"additional","affiliation":[{"name":"Institute of Geobotany\/Plant Ecology, Martin-Luther-University, Gro\u00dfe Steinstra\u00dfe 79\/80, 06108 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Polgar","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical School, University of Pecs, 12 Szigeti Street, 7624 Pecs, Hungary"},{"name":"Janos Szentagothai Research Centre, 20 Ifjusag Street, 7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1208-2969","authenticated-orcid":false,"given":"Laszlo","family":"Szereday","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical School, University of Pecs, 12 Szigeti Street, 7624 Pecs, Hungary"},{"name":"Janos Szentagothai Research Centre, 20 Ifjusag Street, 7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.preghy.2018.05.004","article-title":"The hypertensive disorders of pregnancy: ISSHP classification, diagnosis & management recommendations for international practice","volume":"13","author":"Brown","year":"2018","journal-title":"Pregnancy Hypertens."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/hr.2016.126","article-title":"Epidemiology of hypertensive disorders in pregnancy: Prevalence, risk factors, predictors and prognosis","volume":"40","author":"Umesawa","year":"2017","journal-title":"Hypertens. 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