{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:08:53Z","timestamp":1792642133376,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Liaoning Province","award":["2019-MS-065"],"award-info":[{"award-number":["2019-MS-065"]}]},{"name":"Natural Science Foundation of Liaoning Province","award":["NRF-2017M3A9F6029736"],"award-info":[{"award-number":["NRF-2017M3A9F6029736"]}]},{"name":"Natural Science Foundation of Liaoning Province","award":["NRF-2020M3H1A1075314"],"award-info":[{"award-number":["NRF-2020M3H1A1075314"]}]},{"name":"Natural Science Foundation of Liaoning Province","award":["NRF-2021R1I1A1A01050838"],"award-info":[{"award-number":["NRF-2021R1I1A1A01050838"]}]},{"name":"National Research Foundation of Korea","award":["2019-MS-065"],"award-info":[{"award-number":["2019-MS-065"]}]},{"name":"National Research Foundation of Korea","award":["NRF-2017M3A9F6029736"],"award-info":[{"award-number":["NRF-2017M3A9F6029736"]}]},{"name":"National Research Foundation of Korea","award":["NRF-2020M3H1A1075314"],"award-info":[{"award-number":["NRF-2020M3H1A1075314"]}]},{"name":"National Research Foundation of Korea","award":["NRF-2021R1I1A1A01050838"],"award-info":[{"award-number":["NRF-2021R1I1A1A01050838"]}]},{"name":"ProGen","award":["2019-MS-065"],"award-info":[{"award-number":["2019-MS-065"]}]},{"name":"ProGen","award":["NRF-2017M3A9F6029736"],"award-info":[{"award-number":["NRF-2017M3A9F6029736"]}]},{"name":"ProGen","award":["NRF-2020M3H1A1075314"],"award-info":[{"award-number":["NRF-2020M3H1A1075314"]}]},{"name":"ProGen","award":["NRF-2021R1I1A1A01050838"],"award-info":[{"award-number":["NRF-2021R1I1A1A01050838"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fusobacterium nucleatum is a lesion-associated obligate anaerobic pathogen of destructive periodontal disease; it is also implicated in the progression and severity of colorectal cancer. Four genes (FN0625, FN1055, FN1220, and FN1419) of F. nucleatum are involved in producing hydrogen sulfide (H2S), which plays an essential role against oxidative stress. The molecular functions of Fn1419 are known, but their mechanisms remain unclear. We determined the crystal structure of Fn1419 at 2.5 \u00c5, showing the unique conformation of the PLP-binding site when compared with L-methionine \u03b3-lyase (MGL) proteins. Inhibitor screening for Fn1419 with L-cysteine showed that two natural compounds, gallic acid and dihydromyricetin, selectively inhibit the H2S production of Fn1419. The chemicals of gallic acid, dihydromyricetin, and its analogs containing trihydroxybenzene, were potentially responsible for the enzyme-inhibiting activity on Fn1419. Molecular docking and mutational analyses suggested that Gly112, Pro159, Val337, and Arg373 are involved in gallic acid binding and positioned close to the substrate and pyridoxal-5\u2032-phosphate-binding site. Gallic acid has little effect on the other H2S-producing enzymes (Fn1220 and Fn1055). Overall, we proposed a molecular mechanism underlying the action of Fn1419 from F. nucleatum and found a new lead compound for inhibitor development.<\/jats:p>","DOI":"10.3390\/ijms24021651","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T02:04:45Z","timestamp":1673834685000},"page":"1651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Structural Basis of the Inhibition of L-Methionine \u03b3-Lyase from Fusobacterium nucleatum"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8772-9536","authenticated-orcid":false,"given":"Tingting","family":"Bu","sequence":"first","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"},{"name":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"},{"name":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"},{"name":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"},{"name":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8520-267X","authenticated-orcid":false,"given":"Liying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"},{"name":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Piao","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4813-748X","authenticated-orcid":false,"given":"Nam-Chul","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Gwanak-gu, Seoul 00826, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunshan","family":"Quan","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China"},{"name":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of 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