{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T21:43:48Z","timestamp":1784324628001,"version":"3.55.0"},"reference-count":67,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Poland\u2019s Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["DWD\/3\/10\/2019"],"award-info":[{"award-number":["DWD\/3\/10\/2019"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Next to cow\u2019s milk and eggs, plant foods, i.e., legumes, tree nuts and cereal grains, most often sensitise atopic children. Storage proteins constitutes the most relevant protein fraction of plant foods, causing primary sensitisation. They exhibit strong allergenic properties and immunogenicity. Our goal was to analyse sensitisation to 26 plant storage proteins in a group of 76 children aged 0\u20135 years with chronic symptoms of atopic dermatitis using Allergy Explorer ALEX2 and to discover changes in serum protein\u2013peptide patterns in allergic patients with the use of MALDI-TOF-MS. We reported that 25% of children were allergic to 2S albumins, 19.7% to 7S globulins, 13.2% to 11S globulins and 1.3% to cereal prolamins. The most common allergenic molecules were Ara h 1 (18.4%), Ara h 2 (17.1%), Ara h 6 (15.8%) and Ara h 3 (11.8%) from peanuts, and the mean serum sIgE concentrations in allergic patients were 10.93 kUA\/L, 15.353 kUA\/L, 15.359 kUA\/L and 9.038 kUA\/L, respectively. In children allergic to storage proteins compared to the other patients (both allergic and non-allergic), the cell cycle control protein 50A, testis-expressed sequence 13B, DENN domain-containing protein 5A and SKI family transcriptional corepressor 2 were altered. Our results indicate that the IgE-mediated allergy to storage proteins is a huge problem in a group of young, atopic children, and show the potential of proteomic analysis in the prediction of primary sensitisation to plant foods. It is the next crucial step for understanding the molecular consequences of allergy to storage proteins.<\/jats:p>","DOI":"10.3390\/ijms24021804","type":"journal-article","created":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T02:10:32Z","timestamp":1673921432000},"page":"1804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Changes in Serum Protein\u2013Peptide Patterns in Atopic Children Allergic to Plant Storage Proteins"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8646-1884","authenticated-orcid":false,"given":"Kacper","family":"Packi","sequence":"first","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznan, Poland"},{"name":"AllerGen, Center of Personalized Medicine, 97-300 Piotrkow Trybunalski, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-1066","authenticated-orcid":false,"given":"Joanna","family":"Matysiak","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Calisia University\u2014Kalisz, 62-800 Kalisz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5765-2603","authenticated-orcid":false,"given":"Eliza","family":"Matuszewska","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Br\u0119borowicz","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Pediatric Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9993-1504","authenticated-orcid":false,"given":"Jan","family":"Matysiak","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1111\/cea.13383","article-title":"Age at introduction to complementary solid food and food allergy and sensitization: A systematic review and meta-analysis","volume":"49","author":"Burgess","year":"2019","journal-title":"Clin. Exp. Allergy"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1001\/jamapediatrics.2019.3544","article-title":"Primary Prevention of Cow\u2019s Milk Sensitization and Food Allergy by Avoiding Supplementation with Cow\u2019s Milk Formula at Birth: A Randomized Clinical Trial","volume":"173","author":"Urashima","year":"2019","journal-title":"JAMA Pediatr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Packi, K., Matysiak, J., Klimczak, S., Matuszewska, E., Br\u0119borowicz, A., Pietkiewicz, D., and Matysiak, J. (2022). Analysis of the Serum Profile of Cytokines Involved in the T-Helper Cell Type 17 Immune Response Pathway in Atopic Children with Food Allergy. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19137877"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/j.jaip.2019.11.040","article-title":"Risk Factors for Hen\u2019s Egg Allergy in Europe: EuroPrevall Birth Cohort","volume":"8","author":"Grimshaw","year":"2020","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/cea.12406","article-title":"Which infants with eczema are at risk of food allergy? Results from a population-based cohort","volume":"45","author":"Martin","year":"2015","journal-title":"Clin. Exp. Allergy"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"171","DOI":"10.2174\/1573396314666180613083616","article-title":"Atopic Dermatitis and Food Allergy: A Paediatric Approach","volume":"14","author":"Cartledge","year":"2018","journal-title":"Curr. Pediatr. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1016\/j.jaci.2008.04.032","article-title":"Epidemiologic risks for food allergy","volume":"121","author":"Lack","year":"2008","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.aller.2016.06.006","article-title":"Assessment of IgE-mediated food allergies in children with atopic dermatitis","volume":"45","author":"Mavroudi","year":"2017","journal-title":"Allergol. Immunopathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.jaci.2012.10.034","article-title":"Threshold dose distributions for 5 major allergenic foods in children","volume":"131","author":"Blom","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/all.12341","article-title":"The prevalence and distribution of food sensitization in European adults","volume":"69","author":"Burney","year":"2014","journal-title":"Allergy"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/pai.12563","article-title":"EAACI Molecular Allergology User\u2019s Guide","volume":"27","author":"Matricardi","year":"2016","journal-title":"Pediatr. Allergy Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100118","DOI":"10.1016\/j.waojou.2020.100118","article-title":"The allergenic activity and clinical impact of individual IgE-antibody binding molecules from indoor allergen sources","volume":"13","author":"Caraballo","year":"2020","journal-title":"World Allergy Organ J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1111\/cea.12571","article-title":"What makes an allergen?","volume":"45","author":"Scheurer","year":"2015","journal-title":"Clin. Exp. Allergy"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1007\/BF03362577","article-title":"Stabile pflanzliche Nahrungsmittelallergene: Lipid-Transfer-Proteine","volume":"20","author":"Petersen","year":"2011","journal-title":"Allergo J."},{"key":"ref_15","first-page":"945","article-title":"Seed storage proteins: Structures and biosynthesis","volume":"7","author":"Shewry","year":"1995","journal-title":"Plant Cell"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rasheed, F., Markgren, J., Hedenqvist, M., and Johansson, E. (2020). Modeling to Understand Plant Protein Structure-Function Relationships-Implications for Seed Storage Proteins. Molecules, 25.","DOI":"10.3390\/molecules25040873"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Vissers, Y.M., Blanc, F., Skov, P.S., Johnson, P.E., Rigby, N.M., Przybylski-Nicaise, L., Bernard, H., Wal, J.-M., Ballmer-Weber, B., and Zuidmeer-Jongejan, L. (2011). Effect of heating and glycation on the allergenicity of 2S albumins (Ara h 2\/6) from peanut. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0023998"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1067\/mai.2001.115480","article-title":"Effects of cooking methods on peanut allergenicity","volume":"107","author":"Beyer","year":"2001","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1021\/acs.jafc.1c06828","article-title":"Effect of Processing on the Structure and Allergenicity of Peanut Allergen Ara h 2 Roasted in a Matrix","volume":"70","author":"Chang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.fct.2015.03.005","article-title":"Food processing and allergenicity","volume":"80","author":"Verhoeckx","year":"2015","journal-title":"Food Chem. Toxicol."},{"key":"ref_21","unstructured":"(2022, December 14). Evolutionary Biology of Plant Food Allergens\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/17689599\/."},{"key":"ref_22","unstructured":"(2022, December 14). Allergens of the Cupin Superfamily\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/12440948\/."},{"key":"ref_23","unstructured":"(2022, November 26). ABC Diagnostyki Molekularnej w Alergologii\u2014\u0141ukasz B\u0142a\u017cowski|Ksi\u0105\u017cka w Tezeusz.pl Ksi\u0105\u017cki Promocje, U\u017cywane Ksi\u0105\u017cki, Nowo\u015bci Wydawnicze. Available online: https:\/\/tezeusz.pl\/abc-diagnostyki-molekularnej-w-alergologii-lukasz-blazowski-3950636."},{"key":"ref_24","unstructured":"(2022, November 26). Molecular Allergy Diagnostics|SpringerLink. Available online: https:\/\/link.springer.com\/book\/10.1007\/978-3-319-42499-6."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1021\/jf4022509","article-title":"Ara h 6 Complements Ara h 2 as an Important Marker for IgE Reactivity to Peanut","volume":"62","author":"Koid","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/all.12574","article-title":"IgE recognition patterns in peanut allergy are age dependent: Perspectives of the EuroPrevall study","volume":"70","author":"Lidholm","year":"2015","journal-title":"Allergy"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1111\/cea.12412","article-title":"Diagnostic accuracy of specific IgE to components in diagnosing peanut allergy: A systematic review","volume":"45","author":"Klemans","year":"2015","journal-title":"Clin. Exp. Allergy"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jaci.2012.08.010","article-title":"The diagnostic value of specific IgE to Ara h 2 to predict peanut allergy in children is comparable to a validated and updated diagnostic prediction model","volume":"131","author":"Klemans","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1007\/s11882-014-0426-8","article-title":"Cross-reactivity of peanut allergens","volume":"14","author":"Bublin","year":"2014","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_30","unstructured":"(2022, November 29). Detailed Characterization of Act d 12 and Act d 13 from Kiwi Seeds: Implication in IgE Cross-Reactivity with Peanut and Tree Nuts\u2013Sirvent\u20132014\u2013Allergy\u2014Wiley Online Library. Available online: https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.12486."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.molimm.2018.03.011","article-title":"Tree nut allergens","volume":"100","author":"Geiselhart","year":"2018","journal-title":"Mol. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.molimm.2006.02.016","article-title":"IgE cross-reactivity between the major peanut allergen Ara h 2 and tree nut allergens","volume":"44","author":"Drew","year":"2007","journal-title":"Mol. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1111\/all.13050","article-title":"Ana o 3-specific IgE is a good predictor for clinically relevant cashew allergy in children","volume":"72","author":"Lange","year":"2017","journal-title":"Allergy"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1515\/cclm-2013-0027","article-title":"Isolated IgE reactivity to native walnut vicilin-like protein (nJug r 2) on ISACTM microarray is due to cross-reactive carbohydrate epitopes","volume":"51","author":"Villalta","year":"2013","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1111\/j.1365-2222.2008.02998.x","article-title":"Pistachio vicilin, Pis v 3, is immunoglobulin E-reactive and cross-reacts with the homologous cashew allergen, Ana o 1","volume":"38","author":"Willison","year":"2008","journal-title":"Clin. Exp. Allergy"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1700446","DOI":"10.1002\/mnfr.201700446","article-title":"Allergy to Peanut, Soybean, and Other Legumes: Recent Advances in Allergen Characterization, Stability to Processing and IgE Cross-Reactivity","volume":"62","author":"Cabanillas","year":"2018","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jaci.2013.01.022","article-title":"IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3","volume":"132","author":"Bublin","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_38","unstructured":"(2022, December 05). High-Affinity Allergen-Specific Human Antibodies Cloned from Single IgE B Cell Transcriptomes\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/30545888\/."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.jaci.2013.02.024","article-title":"Sensitization to Cor a 9 and Cor a 14 is highly specific for a hazelnut allergy with objective symptoms in Dutch children and adults","volume":"132","author":"Masthoff","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1159\/000492399","article-title":"Evidence of Cross-Reactivity between Different Seed Storage Proteins from Hazelnut (Corylus avellana) and Walnut (Juglans regia) Using Recombinant Allergen Proteins","volume":"178","author":"Villalta","year":"2019","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_41","unstructured":"(2022, November 29). The 11S Globulin Sin a 2 from Yellow Mustard Seeds Shows IgE Cross-Reactivity with Homologous Counterparts from Tree Nuts and Peanut\u2014PMC, Available online: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3583068\/."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1159\/000321916","article-title":"Anaphylaxis to buckwheat in an atopic child: A risk factor for severe allergy to nuts and seeds?","volume":"156","author":"Varga","year":"2011","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3243","DOI":"10.1016\/j.molimm.2007.01.023","article-title":"Homology modelling and conformational analysis of IgE-binding epitopes of Ara h 3 and other legumin allergens with a cupin fold from tree nuts","volume":"44","author":"Barre","year":"2007","journal-title":"Mol. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ricci, G., Andreozzi, L., Cipriani, F., Giannetti, A., Gallucci, M., and Caffarelli, C. (2019). Wheat Allergy in Children: A Comprehensive Update. Medicina, 55.","DOI":"10.3390\/medicina55070400"},{"key":"ref_45","unstructured":"(2022, December 07). Proteomic Features Characterization of Hymenoptera Venom Allergy|Allergy, Asthma & Clinical Immunology|Full Text. Available online: https:\/\/aacijournal.biomedcentral.com\/articles\/10.1186\/s13223-019-0387-5."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.jaad.2019.10.039","article-title":"The proteomic skin profile of moderate-to-severe atopic dermatitis patients shows an inflammatory signature","volume":"82","author":"Pavel","year":"2020","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yin, J., Wen, Y., Zeng, J., Zhang, Y., Chen, J., Zhang, Y., Han, T., Li, X., Huang, H., and Cai, Y. (2022). CDC50A might be a novel biomarker of epithelial ovarian cancer-initiating cells. BMC Cancer, 22.","DOI":"10.1186\/s12885-022-09953-y"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1159\/000321108","article-title":"A novel immunoassay using recombinant allergens simplifies peanut allergy diagnosis","volume":"154","author":"Codreanu","year":"2011","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"275","DOI":"10.3389\/fphys.2016.00275","article-title":"P4-ATPases as Phospholipid Flippases\u2014Structure, Function, and Enigmas","volume":"7","author":"Andersen","year":"2016","journal-title":"Front. Physiol."},{"key":"ref_50","unstructured":"(2022, December 12). Caspase-Mediated Cleavage of Phospholipid Flippase for Apoptotic Phosphatidylserine Exposure\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/24904167\/."},{"key":"ref_51","unstructured":"(2022, December 12). TMEM30A Deficiency in Endothelial Cells Impairs Cell Proliferation and Angiogenesis\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/30814335\/."},{"key":"ref_52","unstructured":"(2022, December 12). Role for Phospholipid Flippase Complex of ATP8A1 and CDC50A Proteins in Cell Migration\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/23269685\/."},{"key":"ref_53","unstructured":"(2022, December 12). The Epithelial-Mesenchymal Transition Generates Cells with Properties of Stem Cells\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/18485877\/."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1083\/jcb.201008051","article-title":"Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors","volume":"191","author":"Yoshimura","year":"2010","journal-title":"J. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.str.2008.10.014","article-title":"Structural basis for recruitment of Rab6-interacting protein 1 to Golgi via a RUN domain","volume":"17","author":"Recacha","year":"2009","journal-title":"Structure"},{"key":"ref_56","unstructured":"(2022, December 13). Epileptic Encephalopathy Caused by Mutations in the Guanine Nucleotide Exchange Factor DENND5A\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/27866705\/."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1038\/labinvest.3700344","article-title":"Cloning and functional characterization of a new Ski homolog, Fussel-18, specifically expressed in neuronal tissues","volume":"85","author":"Arndt","year":"2005","journal-title":"Lab. Investig."},{"key":"ref_58","unstructured":"(2022, December 13). SKI Pathways Inducing Progression of Human Melanoma|SpringerLink. Available online: https:\/\/link.springer.com\/article\/10.1007\/s10555-005-1576-x."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"(2007). Competition between Ski and CREB-binding Protein for Binding to Smad Proteins in Transforming Growth Factor-\u03b2 Signaling *. J. Biol. Chem., 282, 11365\u201311376. Available online: https:\/\/www.jbc.org\/article\/S0021-9258(20)76716-5\/fulltext.","DOI":"10.1074\/jbc.M700186200"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"D265","DOI":"10.1093\/nar\/gkz991","article-title":"CDD\/SPARCLE: The conserved domain database in 2020","volume":"48","author":"Lu","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1023\/A:1025437329427","article-title":"Molecular and cytogenetic characterization of two azoospermic patients with X-autosome translocation","volume":"20","author":"Lee","year":"2003","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Alvaro, C.G., Braz, J.M., Bernstein, M., Hamel, K.A., Craik, V., Yamanaka, H., and Basbaum, A.I. (2020). Hippocalcin-like 4, a neural calcium sensor, has a limited contribution to pain and itch processing. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0226289"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1093\/chromsci\/bmw167","article-title":"Proteomics: Technologies and Their Applications","volume":"55","author":"Aslam","year":"2017","journal-title":"J. Chromatogr. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Swiatly, A., Horala, A., Hajduk, J., Matysiak, J., Nowak-Markwitz, E., and Kokot, Z.J. (2017). MALDI-TOF-MS analysis in discovery and identification of serum proteomic patterns of ovarian cancer. BMC Cancer, 17.","DOI":"10.1186\/s12885-017-3467-2"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Klupczynska, A., Swiatly, A., Hajduk, J., Matysiak, J., Dyszkiewicz, W., Pawlak, K., and Kokot, Z.J. (2016). Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17040410"},{"key":"ref_66","unstructured":"(2022, December 05). Evaluation of a New Multiplex Assay for Allergy Diagnosis\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/30822401\/."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1111\/all.13810","article-title":"Food Allergy Endotype with High Risk of Severe Anaphylaxis in Children\u2014Monosensitization to Cashew 2S Albumin Ana o 3","volume":"74","author":"Blazowski","year":"2019","journal-title":"Allergy"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/2\/1804\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:07:43Z","timestamp":1760119663000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/2\/1804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,16]]},"references-count":67,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["ijms24021804"],"URL":"https:\/\/doi.org\/10.3390\/ijms24021804","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,16]]}}}