{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:54:26Z","timestamp":1778781266180,"version":"3.51.4"},"reference-count":29,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T00:00:00Z","timestamp":1674172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health of the Czech Republic","award":["MH CZ\u2014DRO (FNOL, 00098892)"],"award-info":[{"award-number":["MH CZ\u2014DRO (FNOL, 00098892)"]}]},{"name":"Ministry of Health of the Czech Republic","award":["IGA_LF_2022_001"],"award-info":[{"award-number":["IGA_LF_2022_001"]}]},{"name":"Ministry of Health of the Czech Republic","award":["IGA_LF_2023_005"],"award-info":[{"award-number":["IGA_LF_2023_005"]}]},{"name":"Internal grant agency of Palack\u00fd University","award":["MH CZ\u2014DRO (FNOL, 00098892)"],"award-info":[{"award-number":["MH CZ\u2014DRO (FNOL, 00098892)"]}]},{"name":"Internal grant agency of Palack\u00fd University","award":["IGA_LF_2022_001"],"award-info":[{"award-number":["IGA_LF_2022_001"]}]},{"name":"Internal grant agency of Palack\u00fd University","award":["IGA_LF_2023_005"],"award-info":[{"award-number":["IGA_LF_2023_005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The treatment outcome in patients with chronic myeloid leukaemia (CML) in blast crisis (BC) is unsatisfactory despite the use of allogeneic stem cell transplantation (ASCT). Moreover, in some patients ASCT is contraindicated, with limited treatment options. We report the case series of two patients with lymphoid BC CML in whom ASCT was not approachable. The first patient developed BC two months after diagnosis in association with dic(7;9)(p11.2;p11.2) and T315I mutation. Blast crisis with central nervous system leukemic involvement and K611N mutation of the SETD2 gene developed abruptly in the second patient five years after ceasing treatment with nilotinib in major molecular response (MMR) at the patient\u2019s request. Both underwent one course of chemotherapy in combination with rituximab and imatinib, followed by dasatinib and interferon \u03b1 (INF\u03b1) treatment in the first and dasatinib alone in the second case. Deep molecular response (DMR; MR 4.0) was achieved within a short time in both cases. It is probable that DMR was caused by a specific immune response to CML cells, described in both agents. The challenging medical condition that prompted these case series, and the subsequent results, suggest a re-visit to the use of a combination of well-known drugs as an area for further investigation.<\/jats:p>","DOI":"10.3390\/ijms24032050","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T02:43:29Z","timestamp":1674182609000},"page":"2050","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Deep Molecular Response Achieved with Chemotherapy, Dasatinib and Interferon \u03b1 in Patients with Lymphoid Blast Crisis of Chronic Myeloid Leukaemia"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-4583","authenticated-orcid":false,"given":"Lucia","family":"Vr\u00e1blov\u00e1","sequence":"first","affiliation":[{"name":"Department of Haemato-Oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0202-245X","authenticated-orcid":false,"given":"Vladim\u00edr","family":"Divok\u00fd","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavla","family":"Ko\u0159alkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate\u0159ina Machov\u00e1","family":"Pol\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Haematology and Blood Transfusion, 120 00 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Kriegov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Immunology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romana","family":"Jansk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Haemato-Oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Grohmann","sequence":"additional","affiliation":[{"name":"Department of Haemato-Oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"Holzerov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Haemato-Oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Papaj\u00edk","sequence":"additional","affiliation":[{"name":"Department of Haemato-Oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Faber","sequence":"additional","affiliation":[{"name":"Department of Haemato-Oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palack\u00fd University, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1038\/s41375-022-01613-1","article-title":"The 5th Edition of the World Health Organization Classification of Haematolymphoid Tumours: Myeloid and Histiocytic\/Dendritic Neoplasms","volume":"36","author":"Khoury","year":"2022","journal-title":"Leukemia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1038\/s41375-020-0776-2","article-title":"European LeukemiaNet 2020 Recommendations for Treating Chronic Myeloid Leukemia","volume":"34","author":"Hochhaus","year":"2020","journal-title":"Leukemia"},{"key":"ref_3","first-page":"275","article-title":"Management and Outcome of CML Blast Crisis: Results from the Randomized CML Study IV","volume":"99","author":"Kalmanti","year":"2014","journal-title":"Haematologica"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1002\/ajh.25628","article-title":"Prognosis of Patients with Chronic Myeloid Leukemia Presenting in Advanced Phase Is Defined Mainly by Blast Count, but Also by Age, Chromosomal Aberrations and Hemoglobin","volume":"94","author":"Lauseker","year":"2019","journal-title":"Am. 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