{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T22:40:19Z","timestamp":1786833619481,"version":"build-2736575974"},"reference-count":23,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,21]],"date-time":"2023-01-21T00:00:00Z","timestamp":1674259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U21A20223"],"award-info":[{"award-number":["U21A20223"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672026"],"award-info":[{"award-number":["31672026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019C02015"],"award-info":[{"award-number":["2019C02015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022C2047"],"award-info":[{"award-number":["2022C2047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LY20C140005"],"award-info":[{"award-number":["LY20C140005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LQ18C140002"],"award-info":[{"award-number":["LQ18C140002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Key Research and Development Program of Zhejiang Province, China","award":["U21A20223"],"award-info":[{"award-number":["U21A20223"]}]},{"name":"the Key Research and Development Program of Zhejiang Province, China","award":["31672026"],"award-info":[{"award-number":["31672026"]}]},{"name":"the Key Research and Development Program of Zhejiang Province, China","award":["2019C02015"],"award-info":[{"award-number":["2019C02015"]}]},{"name":"the Key Research and Development Program of Zhejiang Province, China","award":["2022C2047"],"award-info":[{"award-number":["2022C2047"]}]},{"name":"the Key Research and Development Program of Zhejiang Province, China","award":["LY20C140005"],"award-info":[{"award-number":["LY20C140005"]}]},{"name":"the Key Research and Development Program of Zhejiang Province, China","award":["LQ18C140002"],"award-info":[{"award-number":["LQ18C140002"]}]},{"name":"the Basic Applied Research Project of Zhejiang Province, China","award":["U21A20223"],"award-info":[{"award-number":["U21A20223"]}]},{"name":"the Basic Applied Research Project of Zhejiang Province, China","award":["31672026"],"award-info":[{"award-number":["31672026"]}]},{"name":"the Basic Applied Research Project of Zhejiang Province, China","award":["2019C02015"],"award-info":[{"award-number":["2019C02015"]}]},{"name":"the Basic Applied Research Project of Zhejiang Province, China","award":["2022C2047"],"award-info":[{"award-number":["2022C2047"]}]},{"name":"the Basic Applied Research Project of Zhejiang Province, China","award":["LY20C140005"],"award-info":[{"award-number":["LY20C140005"]}]},{"name":"the Basic Applied Research Project of Zhejiang Province, China","award":["LQ18C140002"],"award-info":[{"award-number":["LQ18C140002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cuticular proteins (CPs) are a large family and perform a variety of functions. However, the physiological roles of cuticle protein 21-like (Cpr21L) in the brown planthopper (Nilaparvata lugens, BPH), one of the most destructive insect pests of rice, are largely unclear. In this study, Cpr21L was revealed to be expressed in both BPH nymphs and adults, and the mRNA expression level was much higher in male adults than female adults. Spatially, the expression of Cpr21L in the testis was higher than in the ovary. The RNA interference (RNAi) of Cpr21L seriously decreased nymph survival, and no individual survived 8 days post-dsCpr21L injection. The RNAi of Cpr21L in adults also decreased the fertility of males, especially in the dsCpr21L\u2642 \u00d7 dsGFP\u2640 group. The average number of eggs laid by one female in this group significantly decreased by 50.1%, and the eggs\u2019 hatchability decreased from 76.5% to 23.8% compared with the control (dsGFP\u2642 \u00d7 dsGFP\u2640). Furthermore, observations under a stereomicroscope showed that the RNAi of Cpr21L severely impaired the development of the testes. Therefore, Cpr21L is essential for the nymphal survival and male fecundity of BPH, thus providing a possible target for pest control.<\/jats:p>","DOI":"10.3390\/ijms24032163","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T02:27:45Z","timestamp":1674440865000},"page":"2163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Novel Cuticular Protein-like Cpr21L Is Essential for Nymph Survival and Male Fecundity in the Brown Planthopper"],"prefix":"10.3390","volume":"24","author":[{"given":"Tongtong","family":"Chen","sequence":"first","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqi","family":"Jiao","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglong","family":"Ye","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangen","family":"Wu","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanxin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiying","family":"Hao","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Yu","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1186\/s13059-014-0521-0","article-title":"Genomes of the rice pest brown planthopper and its endosymbionts reveal complex complementary contributions for host adaptation","volume":"15","author":"Xue","year":"2014","journal-title":"Genome Biol."},{"key":"ref_2","first-page":"415","article-title":"RNAi-mediated silencing of an egg-specific gene Nllet1 results in hatch failure in the brown planthopper","volume":"13","author":"Lu","year":"2022","journal-title":"Pest. Manag. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5175","DOI":"10.1073\/pnas.1716951115","article-title":"A comprehensive omics analysis and functional survey of cuticular proteins in the brown planthopper","volume":"115","author":"Pan","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107674","DOI":"10.1016\/j.jip.2021.107674","article-title":"Antifungal roles of adult-specific cuticular protein genes of the red flour beetle, Tribolium castaneum","volume":"186","author":"Sirasoonthorn","year":"2021","journal-title":"J. Invertebr. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.asd.2004.05.006","article-title":"Design and mechanical properties of insect cuticle","volume":"33","author":"Vincent","year":"2004","journal-title":"Arthropod. Struct. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.ijbiomac.2022.10.279","article-title":"Genome-wide annotation of cuticular protein genes in non-biting midge Propsilocerus akamusi and transcriptome analysis of their response to heavy metal pollution","volume":"223","author":"Liu","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/j.ibmb.2008.05.007","article-title":"Genome-wide identification of cuticular protein genes in the silkworm, Bombyx mori","volume":"38","author":"Futahashi","year":"2008","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cornman, R.S., Togawa, T., Dunn, W.A., He, N., Emmons, A.C., and Willis, J.H. (2008). Annotation and analysis of a large cuticular protein family with the R&R Consensus in Anopheles gambiae. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-22"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/joa.12362","article-title":"Ultrastructure of dragonfly wing veins: Composite structure of fibrous material supplemented by resilin","volume":"227","author":"Appel","year":"2015","journal-title":"J. Anat."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1146\/annurev-cellbio-100616-060439","article-title":"Structural and Mechanistic Insights into Protein Translocation","volume":"33","author":"Rapoport","year":"2017","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6214","DOI":"10.1021\/bi0500980","article-title":"Molecular crosstalk between the nucleotide specificity determinant of the SRP GTPase and the SRP receptor","volume":"44","author":"Shan","year":"2005","journal-title":"Biochemistry"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ceb.2016.04.009","article-title":"Toward a structural understanding of co-translational protein translocation","volume":"41","author":"Voorhees","year":"2016","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"153","DOI":"10.3389\/fcell.2019.00153","article-title":"A kinetic view of membrane traffic pathways can transcend the classical view of golgi compartments","volume":"7","author":"Pantazopoulou","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.cell.2020.03.031","article-title":"A translocation pathway for vesicle-mediated unconventional protein secretion","volume":"181","author":"Zhang","year":"2020","journal-title":"Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1016\/S2095-3119(20)63248-8","article-title":"Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust, Locusta migratoria","volume":"20","author":"Zhao","year":"2021","journal-title":"J. Integr. Agric."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"114573","DOI":"10.1016\/j.envpol.2020.114573","article-title":"Etoxazole induces testicular malfunction in mice by dysregulating mitochondrial function and calcium homeostasis","volume":"263","author":"Ham","year":"2020","journal-title":"Env. Pollut."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.3762\/bjnano.7.115","article-title":"Functional diversity of resilin in Arthropoda","volume":"7","author":"Michels","year":"2016","journal-title":"Beilstein. J. Nanotech."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms2004","article-title":"Mechanism of resilin elasticity","volume":"3","author":"Qin","year":"2012","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1002\/jmor.21364","article-title":"Discovery of a complex sclerotized sperm pump in Monotomidae (Coleoptera): Functional morphology and phylogenetic value","volume":"282","author":"Jaloszynski","year":"2021","journal-title":"J. Morphol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1038\/s42003-021-02396-4","article-title":"Cuticular modified air sacs underlie white coloration in the olive fruit fly, Bactrocera oleae","volume":"4","author":"Rebora","year":"2021","journal-title":"Commun. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yuan, M., Lu, Y., Xun, Z., Hu, W., Muhammad, S., Sha, Z., Jin, B.R., Li, J., and Wang, X.W.J.P.O. (2014). Selection and evaluation of potential reference genes for gene expression analysis in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) using reverse-transcription quantitative PCR. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0086503"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11413","DOI":"10.1038\/srep11413","article-title":"Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant","volume":"5","author":"Huang","year":"2015","journal-title":"Sci. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/3\/2163\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:13:03Z","timestamp":1760119983000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/3\/2163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,21]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ijms24032163"],"URL":"https:\/\/doi.org\/10.3390\/ijms24032163","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,21]]}}}