{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T18:45:05Z","timestamp":1783968305896,"version":"3.55.0"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,22]],"date-time":"2023-01-22T00:00:00Z","timestamp":1674345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Program for fundamental research SSC RF\u2014IBMP RAS 65.4"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endometriosis of the cervix is a rare form of genital endometriosis, which is characterized by the appearance of tissue on the vaginal part of the cervix, similar to the tissue of the mucous membrane of the uterine cavity. We describe a clinical case in which we compared the content of cytoskeletal proteins, H3 histone modifications and DNA methylation (total and 5-hydroxymethylcytosine content) in the eutopic endometrium and in tissue from endometriosis foci on the cervix. The patient had elevated levels of estradiol, interleukin-1\u03b2 and interleukin-8. At the cellular level, the content of tubulin and the marker of stable microtubules were reduced in the ectopic endometrium (by 45% and 37%, p &lt; 0.05, respectively), but the alpha-actinin-1 content was increased (by 75%, p &lt; 0.05) with an increase in the expression of its gene. At the same time, the total level of DNA methylation in the endometriotic focus was reduced by more than 2 times with the accumulation of the intermediate product 5-hydroxymethylcytosine (the content increased by more than 3 times), probably due to an increase in the content of tet methylcytosine dioxygenase 1 (more than 4 times).<\/jats:p>","DOI":"10.3390\/ijms24032184","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T02:27:45Z","timestamp":1674440865000},"page":"2184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Endometriosis of the Cervix: A Rare Clinical Case with the Possibility of Comparing the Eutopic and Ectopic Endometrium at the Cellular Level"],"prefix":"10.3390","volume":"24","author":[{"given":"Konstantin A.","family":"Toniyan","sequence":"first","affiliation":[{"name":"Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 121352 Moscow, Russia"},{"name":"Cell Biophysics Lab., State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Yu.","family":"Gorbacheva","sequence":"additional","affiliation":[{"name":"Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 121352 Moscow, Russia"},{"name":"Cell Biophysics Lab., State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valery V.","family":"Boyarintsev","sequence":"additional","affiliation":[{"name":"Emergency and Extreme Medicine Department, FGBU DPO CGMA UDP RF, 121359 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0125-5876","authenticated-orcid":false,"given":"Irina V.","family":"Ogneva","sequence":"additional","affiliation":[{"name":"Cell Biophysics Lab., State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1016\/S0140-6736(04)17403-5","article-title":"Endometriosis","volume":"364","author":"Giudice","year":"2004","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1056\/NEJMra0804690","article-title":"Endometriosis","volume":"360","author":"Bulun","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1093\/humupd\/dmz005","article-title":"Endometriosis and nuclear receptors","volume":"25","author":"Yilmaz","year":"2019","journal-title":"Hum. Reprod Update"},{"key":"ref_4","first-page":"5974693","article-title":"Expression Pattern of G-Protein-Coupled Estrogen Receptor in Myometrium of Uteri with and without Adenomyosis","volume":"2017","author":"Li","year":"2017","journal-title":"Biomed. Res. Int."},{"key":"ref_5","first-page":"2196024","article-title":"Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis","volume":"2020","year":"2020","journal-title":"Biomed. Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1080\/01443610410001696978","article-title":"Persistent post-coital bleeding due to cervical endometriosis","volume":"24","author":"Doshi","year":"2004","journal-title":"J. Obstet. Gynaecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1177\/1933719116660847","article-title":"Genes Linked to Endometriosis by GWAS Are Integral to Cytoskeleton Regulation and Suggests That Mesothelial Barrier Homeostasis Is a Factor in the Pathogenesis of Endometriosis","volume":"24","author":"Albertsen","year":"2017","journal-title":"Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Toniyan, K.A., Povorova, V.V., Gorbacheva, E.Y., Boyarintsev, V.V., and Ogneva, I.V. (2021). Organization of the Cytoskeleton in Ectopic Foci of the Endometrium with Rare Localization. Biomedicines, 9.","DOI":"10.3390\/biomedicines9080998"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/S0046-8177(98)90021-1","article-title":"Pleuro-pulmonary endometriosis and pulmonary ectopic deciduosis: A clinicopathologic and immunohistochemical study of 10 cases with emphasis on diagnostic pitfalls","volume":"29","author":"Flieder","year":"1998","journal-title":"Hum. Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e432","DOI":"10.1002\/rcr2.432","article-title":"Pulmonary endometriosis: A rare cause of hydropneumothorax","volume":"7","author":"Alzayer","year":"2019","journal-title":"Respirol. Case Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1159\/000095776","article-title":"Nasolacrimal endometriosis. A case report","volume":"38","author":"Oner","year":"2006","journal-title":"Ophthalmic. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1097\/IOP.0b013e31815c9053","article-title":"Presumed nasolacrimal endometriosis","volume":"24","author":"Trkolu","year":"2008","journal-title":"Ophthalmic. Plast Reconstr. Surg."},{"key":"ref_13","first-page":"64","article-title":"Cervical endometriosis, a case presenting with intractable spotting","volume":"7","author":"Ata","year":"2005","journal-title":"MedGenMed"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.fertnstert.2011.06.041","article-title":"Interleukin-1 induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometrioma","volume":"96","author":"Kao","year":"2011","journal-title":"Fertil. Steril."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/molehr\/6.3.269","article-title":"Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1beta","volume":"6","author":"Lebovic","year":"2000","journal-title":"Mol. Hum. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0015-0282(00)01630-7","article-title":"Immunobiology of endometriosis","volume":"75","author":"Lebovic","year":"2001","journal-title":"Fertil. Steril."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/humupd\/6.1.45","article-title":"Vascular endothelial growth factor and endometriotic angiogenesis","volume":"6","author":"McLaren","year":"2000","journal-title":"Hum. Reprod. Update"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.fertnstert.2007.12.067","article-title":"Expression of interleukin-8 and monocyte chemotactic protein 1 in women with endometriosis","volume":"91","author":"Ulukus","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e12622","DOI":"10.1111\/aji.12622","article-title":"Abnormal peritoneal regulation of chemokine activation-The role of IL-8 in pathogenesis of endometriosis","volume":"77","author":"Sikora","year":"2017","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1093\/molehr\/gap045","article-title":"Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells","volume":"15","author":"Flamini","year":"2009","journal-title":"Mol. Hum. Reprod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.fertnstert.2008.10.014","article-title":"Endometrial stromal cells from women with endometriosis reveal peculiar migratory behavior in response to ovarian steroids","volume":"93","author":"Gentilini","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1042\/bst0150796","article-title":"The structure and function of alpha-actinin","volume":"15","author":"Baron","year":"1987","journal-title":"Biochem. Soc. Trans."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1006\/jsbi.2002.4465","article-title":"Towards a complete atomic structure of spectrin family proteins","volume":"137","author":"Broderick","year":"2002","journal-title":"J. Struct. Biol."},{"key":"ref_24","first-page":"62","article-title":"Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene","volume":"47","author":"Youssoufian","year":"1990","journal-title":"Am. J. Hum. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6263","DOI":"10.1016\/S0021-9258(18)42690-7","article-title":"Mutually exclusive splicing of calcium-binding domain exons in chick alpha-actinin","volume":"267","author":"Waites","year":"1992","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1111\/j.1432-1033.1992.tb17483.x","article-title":"A chick skeletal-muscle alpha-actinin gene gives rise to two alternatively spliced isoforms which differ in the EF-hand Ca(2+)-binding domain","volume":"210","author":"Parr","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.ajhg.2013.01.015","article-title":"ACTN1 mutations cause congenital macrothrombocytopenia","volume":"92","author":"Kunishima","year":"2013","journal-title":"Am. J. Hum. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"O\u2019Sullivan, L.R., Cahill, M.R., and Young, P.W. (2021). The Importance of Alpha-Actinin Proteins in Platelet Formation and Function, and Their Causative Role in Congenital Macrothrombocytopenia. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22179363"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1158\/1078-0432.CCR-11-3077","article-title":"Characterization of gene amplification-driven SKP2 overexpression in myxofibrosarcoma: Potential implications in tumor progression and therapeutics","volume":"18","author":"Li","year":"2012","journal-title":"Clin. Cancer Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kovac, B., Mkel, T.P., and Vallenius, T. (2018). Increased -actinin-1 destabilizes E-cadherin-based adhesions and associates with poor prognosis in basal-like breast cancer. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196986"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3015","DOI":"10.21037\/jtd.2019.07.50","article-title":"miR-30b-5p acts as a tumor suppressor microRNA in esophageal squamous cell carcinoma","volume":"11","author":"Xu","year":"2019","journal-title":"J. Thorac. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1111\/j.1471-0528.2000.tb11116.x","article-title":"Site of origin of epithelial ovarian cancer: The endometriosis connection","volume":"107","author":"Vercellini","year":"2000","journal-title":"BJOG"},{"key":"ref_33","first-page":"7689","article-title":"Association between ovarian cancer and advanced endometriosis","volume":"15","author":"Matalliotakis","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"113316","DOI":"10.1016\/j.ejmech.2021.113316","article-title":"Discovery of novel N-benzylbenzamide derivatives as tubulin polymerization inhibitors with potent antitumor activities","volume":"216","author":"Zhu","year":"2021","journal-title":"Eur. J. Med. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"173853","DOI":"10.1016\/j.ejphar.2021.173853","article-title":"Synthetic cyclopenta[b]indoles exhibit antineoplastic activity by targeting microtubule dynamics in acute myeloid leukemia cells","volume":"894","author":"Vicari","year":"2021","journal-title":"Eur. J. Pharmacol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e181","DOI":"10.1093\/nar\/gkq684","article-title":"Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA","volume":"38","author":"Szwagierczak","year":"2010","journal-title":"Nucleic. Acids. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1126\/science.1210597","article-title":"Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine","volume":"333","author":"Ito","year":"2011","journal-title":"Science"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/bies.201600178","article-title":"DNA demethylation pathways: Additional players and regulators","volume":"39","author":"Bochtler","year":"2017","journal-title":"Bioessays"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1021\/ja210273w","article-title":"Effects of DNA methylation on the structure of nucleosomes","volume":"134","author":"Lee","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Liu, N.T., Perng, C.L., Chou, Y.C., Ko, P.S., Lin, Y.J., Lin, Y.C., Chang, C.C., Wang, Y.C., Shang, H.S., and Chao, T.K. (2021). Loss of ten-eleven translocation 1 (TET1) expression as a diagnostic and prognostic biomarker of endometrial carcinoma. 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