{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T19:08:43Z","timestamp":1774638523236,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T00:00:00Z","timestamp":1674518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Czech Sciences Foundation","award":["CSF Bilateral AT-CZ 21-38204L"],"award-info":[{"award-number":["CSF Bilateral AT-CZ 21-38204L"]}]},{"name":"Czech Sciences Foundation","award":["I 5215"],"award-info":[{"award-number":["I 5215"]}]},{"name":"Czech Sciences Foundation","award":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000754"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000754"]}]},{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youth and Sports of the Czech Republic, ERDF\/ESF project Nanotechnologies for Future","doi-asserted-by":"publisher","award":["CSF Bilateral AT-CZ 21-38204L"],"award-info":[{"award-number":["CSF Bilateral AT-CZ 21-38204L"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youth and Sports of the Czech Republic, ERDF\/ESF project Nanotechnologies for Future","doi-asserted-by":"publisher","award":["I 5215"],"award-info":[{"award-number":["I 5215"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youth and Sports of the Czech Republic, ERDF\/ESF project Nanotechnologies for Future","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000754"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000754"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Motivated by the clinical success of gold(I) metallotherapeutic Auranofin in the effective treatment of both inflammatory and cancer diseases, we decided to prepare, characterize, and further study the [Au(kin)(PPh3)] complex (1), where Hkin = kinetin, 6-furfuryladenine, for its in vitro anti-cancer and anti-inflammatory activities. The results revealed that the complex (1) had significant in vitro cytotoxicity against human cancer cell lines (A2780, A2780R, PC-3, 22Rv1, and THP-1), with IC50 \u2248 1\u20135 \u03bcM, which was even significantly better than that for the conventional platinum-based drug Cisplatin while comparable with Auranofin. Although its ability to inhibit transcription factor NF-\u03baB activity did not exceed the comparative drug Auranofin, it has been found that it is able to positively influence peroxisome-proliferator-activated receptor-gamma (PPAR\u03b3), and as a consequence of this to have the impact of moderating\/reducing inflammation. The cellular effects of the complex (1) in A2780 cancer cells were also investigated by cell cycle analysis, induction of apoptosis, intracellular ROS production, activation of caspases 3\/7 and disruption of mitochondrial membrane potential, and shotgun proteomic analysis. Proteomic analysis of R2780 cells treated with complex (1) and starting compounds revealed possible different places of the effect of the studied compounds. Moreover, the time-dependent cellular accumulation of copper was studied by means of the mass spectrometry study with the aim of exploring the possible mechanisms responsible for its biological effects.<\/jats:p>","DOI":"10.3390\/ijms24032293","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T03:53:18Z","timestamp":1674618798000},"page":"2293","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Gold(I) Complex with Plant Hormone Kinetin Shows Promising In Vitro Anticancer and PPAR\u03b3 Properties"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5890-7874","authenticated-orcid":false,"given":"Zden\u011bk","family":"Tr\u00e1vn\u00ed\u010dek","sequence":"first","affiliation":[{"name":"Czech Advanced Technology and Research Institute, Regional Centre of Advanced Technologies and Materials, Palack\u00fd University, \u0160lechtitel\u016f 27, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3351-1192","authenticated-orcid":false,"given":"J\u00e1n","family":"Van\u010do","sequence":"additional","affiliation":[{"name":"Czech Advanced Technology and Research Institute, Regional Centre of Advanced Technologies and Materials, Palack\u00fd University, \u0160lechtitel\u016f 27, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Belza","sequence":"additional","affiliation":[{"name":"Czech Advanced Technology and Research Institute, Regional Centre of Advanced Technologies and Materials, Palack\u00fd University, \u0160lechtitel\u016f 27, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0975-1671","authenticated-orcid":false,"given":"Jan","family":"Ho\u0161ek","sequence":"additional","affiliation":[{"name":"Czech Advanced Technology and Research Institute, Regional Centre of Advanced Technologies and Materials, Palack\u00fd University, \u0160lechtitel\u016f 27, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3938-3585","authenticated-orcid":false,"given":"Zden\u011bk","family":"Dvo\u0159\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Faculty of Science, Palack\u00fd University, \u0160lechtitel\u016f 27, 78371 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9","family":"Lenobel","sequence":"additional","affiliation":[{"name":"Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences, Faculty of Science, Palack\u00fd University, \u0160lechtitel\u016f 27, 78371 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Popa","sequence":"additional","affiliation":[{"name":"Czech Advanced Technology and Research Institute, Regional Centre of Advanced Technologies and Materials, Palack\u00fd University, \u0160lechtitel\u016f 27, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4336-7924","authenticated-orcid":false,"given":"Karel","family":"\u0160mejkal","sequence":"additional","affiliation":[{"name":"Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palack\u00e9ho t\u0159. 1946\/1, 61242 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9792-3420","authenticated-orcid":false,"given":"Pavel","family":"Uhrin","sequence":"additional","affiliation":[{"name":"Institute of Vascular Biology and Thrombosis Research, Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"378","DOI":"10.2165\/00003495-198427050-00002","article-title":"Auranofin: A preliminary review of its pharmacological properties and therapeutic use in rheumatoid arthritis","volume":"27","author":"Chaffman","year":"1984","journal-title":"Drugs"},{"key":"ref_2","first-page":"1111","article-title":"Upgrade of an old drug: Auranofin in innovative cancer therapies to overcome drug resistance and to increase drug effectiveness","volume":"42","author":"Gamberi","year":"2020","journal-title":"Med. 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