{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:47:59Z","timestamp":1790689679136,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T00:00:00Z","timestamp":1674777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The 18 kDa translocator protein (TSPO\/PBR) is a multifunctional evolutionary highly conserved outer mitochondrial membrane protein. Decades of research has reported an obligatory role of TSPO\/PBR in both mitochondrial cholesterol transport and, thus, steroid production. However, the strict dependency of steroidogenesis on TSPO\/PBR has remained controversial. The aim of this study was to provide insight into the steroid profile in complete C57BL\/6-Tspotm1GuWu(GuwiyangWurra)-knockout male mice (TSPO-KO) under basal conditions. The steroidome in the brain, adrenal glands, testes and plasma was measured by gas chromatography coupled to tandem mass spectrometry (GC-MS\/MS). We found that steroids present in wild-type (WT) mice were also detected in TSPO-KO mice, including pregnenolone (PREG), progestogens, mineralo-glucocorticosteroids and androgens. The concentrations of PREG and most metabolites were similar between genotypes, except a significant decrease in the levels of the 5\u03b1-reduced metabolites of progesterone (PROG) in adrenal glands and plasma and of the 5\u03b1-reduced metabolites of corticosterone (B) in plasma in TSPO-KO compared to WT animals, suggesting other regulatory functions for the TSPO\/PBR. The expression levels of the voltage-dependent anion-selective channel (VDAC-1), CYP11A1 and 5\u03b1-reductase were not significantly different between both groups. Thus, the complete deletion of the tspo gene in male mice does not impair de novo steroidogenesis in vivo.<\/jats:p>","DOI":"10.3390\/ijms24032474","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T04:31:27Z","timestamp":1675053087000},"page":"2474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Comprehensive Steroidome in Complete TSPO\/PBR Knockout Mice under Basal Conditions"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9603-0586","authenticated-orcid":false,"given":"Philippe","family":"Liere","sequence":"first","affiliation":[{"name":"Disease and Hormones of the Nervous System, U1195 Inserm-Universit\u00e9 Paris Saclay, 80 rue du G\u00e9n\u00e9ral Leclerc, 94276 Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4278-8372","authenticated-orcid":false,"given":"Guo-Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Australian Nuclear Science and Technology Organisation (ANSTO), Kirrawee, NSW 2232, Australia"},{"name":"Faculty of Medicine and Health, Medical Imaging Sciences, Brain and Mind Centre, University of Sydney, Camperdown, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5417-5429","authenticated-orcid":false,"given":"Antoine","family":"Pianos","sequence":"additional","affiliation":[{"name":"Disease and Hormones of the Nervous System, U1195 Inserm-Universit\u00e9 Paris Saclay, 80 rue du G\u00e9n\u00e9ral Leclerc, 94276 Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8024-7772","authenticated-orcid":false,"given":"Ryan J.","family":"Middleton","sequence":"additional","affiliation":[{"name":"Australian Nuclear Science and Technology Organisation (ANSTO), Kirrawee, NSW 2232, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard B.","family":"Banati","sequence":"additional","affiliation":[{"name":"Australian Nuclear Science and Technology Organisation (ANSTO), Kirrawee, NSW 2232, Australia"},{"name":"Faculty of Medicine and Health, Medical Imaging Sciences, Brain and Mind Centre, University of Sydney, Camperdown, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2181-4612","authenticated-orcid":false,"given":"Yvette","family":"Akwa","sequence":"additional","affiliation":[{"name":"Disease and Hormones of the Nervous System, U1195 Inserm-Universit\u00e9 Paris Saclay, 80 rue du G\u00e9n\u00e9ral Leclerc, 94276 Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.pneurobio.2003.09.004","article-title":"Steroid hormones and neurosteroids in normal and pathological aging of the nervous system","volume":"71","author":"Schumacher","year":"2003","journal-title":"Prog. 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