{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:02:19Z","timestamp":1782936139920,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T00:00:00Z","timestamp":1674864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["P 32534"],"award-info":[{"award-number":["P 32534"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Transient receptor potential cation channel subfamily A member 1 (TRPA1), an ion channel primarily expressed on sensory neurons, can be activated by substances occurring during myocardial infarction. Aims were to investigate whether activation, inhibition, or absence of TRPA1 affects infarcts and to explore underlying mechanisms. In the context of myocardial infarction, rats received a TRPA1 agonist, an antagonist, or vehicle at different time points, and infarct size was assessed. Wild type and TRPA1 knockout mice were also compared in this regard. In vitro, sensory neurons were co-cultured with cardiomyocytes and subjected to a model of ischemia-reperfusion. Although there was a difference between TRPA1 activation or inhibition in vivo, no experimental group was different to control animals in infarct size, which also applies to animals lacking TRPA1. In vitro, survival probability of cardiomyocytes challenged by ischemia-reperfusion increased from 32.8% in absence to 45.1% in presence of sensory neurons, which depends, at least partly, on TRPA1. This study raises doubts about whether TRPA1 is a promising target to reduce myocardial damage within a 24 h period. The results are incompatible with relevant enlargements of infarcts by TRPA1 activation or inhibition, which argues against adverse effects when TRPA1 is targeted for other indications.<\/jats:p>","DOI":"10.3390\/ijms24032516","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T04:58:25Z","timestamp":1675054705000},"page":"2516","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["TRPA1 as Target in Myocardial Infarction"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7165-7104","authenticated-orcid":false,"given":"Clara","family":"Hoebart","sequence":"first","affiliation":[{"name":"Center for Physiology and Pharmacology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4652-1998","authenticated-orcid":false,"given":"Attila","family":"Kiss","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Translational Surgery, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6149-8736","authenticated-orcid":false,"given":"Patrick M.","family":"Pilz","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Translational Surgery, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5465-8391","authenticated-orcid":false,"given":"Petra L.","family":"Szabo","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Translational Surgery, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4641-7202","authenticated-orcid":false,"given":"Bruno K.","family":"Podesser","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Translational Surgery, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3811-7066","authenticated-orcid":false,"given":"Michael J. M.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Center for Physiology and Pharmacology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3398-0442","authenticated-orcid":false,"given":"Stefan","family":"Heber","sequence":"additional","affiliation":[{"name":"Center for Physiology and Pharmacology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,28]]},"reference":[{"key":"ref_1","unstructured":"(2023, January 22). Eurostat Causes of Death\u2014Deaths by Country of Residence and Occurrence 2020. Available online: https:\/\/ec.europa.eu\/eurostat\/statistics-explained\/index.php?title=Causes_of_death_statistics#Main_causes_of_death_by_country_in_2019."},{"key":"ref_2","unstructured":"National Center for Health Statistics, and Heron, M. (2021). Deaths: Leading Causes for 2018."},{"key":"ref_3","unstructured":"(2022, November 25). Fourth Universal Definition of Myocardial Infarction (2018). Available online: https:\/\/reader.elsevier.com\/reader\/sd\/pii\/S0735109718369419?token=75DF863FD74EA5A49E1EAC1497159F0235787C4590C69EB94D3E0BE79C6728BFA0F5E005B81FAC2EF2E19AE190A84E95&originRegion=eu-west-1&originCreation=20221125102729."},{"key":"ref_4","unstructured":"(2022, November 25). Evolving Therapies for Myocardial Ischemia\/Reperfusion Injury. Available online: https:\/\/reader.elsevier.com\/reader\/sd\/pii\/S0735109715006695?token=878701DF723F5D0AACFB516BA6B9BBE7AC17C390F0ED728DE610BF461CF61A78E707F4D96587C4B05A1C3F439585CB56&originRegion=eu-west-1&originCreation=20221125102849."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1152\/jn.00524.2018","article-title":"TRPA1: A Molecular View","volume":"121","author":"Meents","year":"2019","journal-title":"J. Neurophysiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/nchembio.640","article-title":"TRPA1 Underlies a Sensing Mechanism for O2","volume":"7","author":"Takahashi","year":"2011","journal-title":"Nat. Chem. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"464","DOI":"10.2183\/pjab.93.028","article-title":"TRP Channels in Oxygen Physiology: Distinctive Functional Properties and Roles of TRPA1 in O2 Sensing","volume":"93","author":"Mori","year":"2017","journal-title":"Proc. Jpn. Acad. Ser. B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1113\/JP270935","article-title":"TRPA1 Channels: Molecular Sentinels of Cellular Stress and Tissue Damage","volume":"594","author":"Viana","year":"2016","journal-title":"J. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.3389\/fphar.2019.01253","article-title":"The TRPA1 Channel in the Cardiovascular System: Promising Features and Challenges","volume":"10","author":"Wang","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Nilius, B., and Flockerzi, V. (2014). Mammalian Transient Receptor Potential (TRP) Cation Channels: Volume II, Springer International Publishing. Handbook of Experimental Pharmacology.","DOI":"10.1007\/978-3-319-05161-1"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"324","DOI":"10.3389\/fphys.2012.00324","article-title":"TRP Channels: Sensors and Transducers of Gasotransmitter Signals","volume":"3","author":"Takahashi","year":"2012","journal-title":"Front. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Barabas, M.E., Kossyreva, E.A., and Stucky, C.L. (2012). TRPA1 Is Functionally Expressed Primarily by IB4-Binding, Non-Peptidergic Mouse and Rat Sensory Neurons. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047988"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1944","DOI":"10.1038\/s41467-019-09770-1","article-title":"Identification of Peripheral Neural Circuits That Regulate Heart Rate Using Optogenetic and Viral Vector Strategies","volume":"10","author":"Rajendran","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e13659","DOI":"10.1111\/apha.13659","article-title":"No Functional TRPA1 in Cardiomyocytes","volume":"232","author":"Hoebart","year":"2021","journal-title":"Acta Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1097\/ALN.0000000000001377","article-title":"Transient Receptor Potential Ankyrin 1 Activation within the Cardiac Myocyte Limits Ischemia\u2013Reperfusion Injury in Rodents","volume":"125","author":"Lu","year":"2016","journal-title":"Anesthesiology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"H889","DOI":"10.1152\/ajpheart.00106.2018","article-title":"TRPA1 Channel Contributes to Myocardial Ischemia-Reperfusion Injury","volume":"316","author":"Conklin","year":"2019","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1080\/19336950.2019.1623591","article-title":"Stimulation of TRPA1 Attenuates Ischemia-Induced Cardiomyocyte Cell Death through an ENOS-Mediated Mechanism","volume":"13","author":"Andrei","year":"2019","journal-title":"Channels"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1097\/FJC.0000000000000783","article-title":"Inhibition of TRPA1 Promotes Cardiac Repair in Mice After Myocardial Infarction","volume":"75","author":"Li","year":"2020","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e6408352","DOI":"10.1155\/2019\/6408352","article-title":"TRPA1 Promotes Cardiac Myofibroblast Transdifferentiation after Myocardial Infarction Injury via the Calcineurin-NFAT-DYRK1A Signaling Pathway","volume":"2019","author":"Li","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00424-016-1922-6","article-title":"Cardioprotection by Remote Ischemic Conditioning and Its Signal Transduction","volume":"469","author":"Kleinbongard","year":"2017","journal-title":"Pfl\u00fcg. Arch. Eur. J. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wu, C.-K., Lin, J.-F., Lee, T.-S., Kou, Y.R., and Tarng, D.-C. (2021). Role of TRPA1 in Tissue Damage and Kidney Disease. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073415"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1093\/ajh\/hpaa162","article-title":"Knockout of Trpa1 Exacerbates Renal Ischemia\u2013Reperfusion Injury With Classical Activation of Macrophages","volume":"34","author":"Ma","year":"2021","journal-title":"Am. J. Hypertens."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wu, C.-K., Wu, C.-L., Lee, T.-S., Kou, Y.R., and Tarng, D.-C. (2021). Renal Tubular Epithelial TRPA1 Acts as An Oxidative Stress Sensor to Mediate Ischemia-Reperfusion-Induced Kidney Injury through MAPKs\/NF-\u039aB Signaling. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052309"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/s41419-020-02863-6","article-title":"TRPA1 Mediates Damage of the Retina Induced by Ischemia and Reperfusion in Mice","volume":"11","author":"Adembri","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.exer.2016.11.015","article-title":"Cannabinoid Receptors and TRPA1 on Neuroprotection in a Model of Retinal Ischemia","volume":"154","author":"Pandolfo","year":"2017","journal-title":"Exp. Eye Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e35316","DOI":"10.7554\/eLife.35316","article-title":"Neuroprotective Effects of TRPA1 Channels in the Cerebral Endothelium Following Ischemic Stroke","volume":"7","author":"Pires","year":"2018","journal-title":"eLife"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lajoso, W., Flower, G., Giacco, V., Kaul, A., La Mache, C., Br\u0103ban, A., Roxas, A., and Hamilton, N.B. (2021). Transient Receptor Potential Ankyrin-1 (TRPA1) Block Protects against Loss of White Matter Function during Ischaemia in the Mouse Optic Nerve. Pharmaceuticals, 14.","DOI":"10.3390\/ph14090909"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/nature16519","article-title":"Proton-Gated Ca2+-Permeable TRP Channels Damage Myelin in Conditions Mimicking Ischaemia","volume":"529","author":"Hamilton","year":"2016","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1007\/s10571-008-9268-z","article-title":"Formation of Neuromuscular Junctions and Synthesis of Sensory Neuropeptides in the Co-Cultures of Dorsal Root Ganglion and Cardiac Myocytes","volume":"28","author":"Liu","year":"2008","journal-title":"Cell. Mol. Neurobiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.bbrc.2012.05.071","article-title":"Impact of Hypoxia, Simulated Ischemia and Reperfusion in HL-1 Cells on the Expression of FKBP12\/FKBP12.6 and Intracellular Calcium Dynamics","volume":"422","author":"Li","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"E207","DOI":"10.1152\/ajpendo.00215.2010","article-title":"Methylglyoxal Increases Cardiomyocyte Ischemia-Reperfusion Injury via Glycative Inhibition of Thioredoxin Activity","volume":"299","author":"Wang","year":"2010","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1177\/1074248414524480","article-title":"Investigating Mitochondrial Metabolism in Contracting HL-1 Cardiomyocytes Following Hypoxia and Pharmacological HIF Activation Identifies HIF-Dependent and Independent Mechanisms of Regulation","volume":"19","author":"Ambrose","year":"2014","journal-title":"J. Cardiovasc. Pharmacol. Ther."},{"key":"ref_33","first-page":"4125","article-title":"Lidocaine Protects H9c2 Cells from Hypoxia-induced Injury through Regulation of the MAPK\/ERK\/NF-\u03baB Signaling Pathway","volume":"18","author":"Jin","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"BSR20181613","DOI":"10.1042\/BSR20181613","article-title":"PLCE1 Promotes Myocardial Ischemia\u2013Reperfusion Injury in H\/R H9c2 Cells and I\/R Rats by Promoting Inflammation","volume":"39","author":"Li","year":"2019","journal-title":"Biosci. Rep."},{"key":"ref_35","first-page":"2153","article-title":"Effects of Nuclear Respiratory Factor-1 on Apoptosis and Mitochondrial Dysfunction Induced by Cobalt Chloride in H9C2 Cells","volume":"19","author":"Niu","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1536\/ihj.16-091","article-title":"Cyclosporin A Protects H9c2 Cells Against Chemical Hypoxia-Induced Injury via Inhibition of MAPK Signaling Pathway","volume":"57","author":"Wang","year":"2016","journal-title":"Int. Heart. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/S0008-6363(02)00461-3","article-title":"Effect of Inhibition of Na+\/Ca2+ Exchanger at the Time of Myocardial Reperfusion on Hypercontracture and Cell Death","volume":"55","author":"Inserte","year":"2002","journal-title":"Cardiovasc. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"419","DOI":"10.3389\/fncel.2017.00419","article-title":"Peripheral Mechanisms of Ischemic Myalgia","volume":"11","author":"Queme","year":"2017","journal-title":"Front. Cell. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.yjmcc.2019.12.003","article-title":"Inhibition of DRG-TRPV1 Upregulation in Myocardial Ischemia Contributes to Exogenous Cardioprotection","volume":"138","author":"Xu","year":"2020","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"122","DOI":"10.2174\/1871529X19666190912152041","article-title":"Protective Effects of TRPV1 Activation Against Cardiac Ischemia\/Reperfusion Injury Is Blunted by Diet-Induced Obesity","volume":"20","author":"Zhong","year":"2020","journal-title":"Cardiovasc. Hematol. Disord. Drug Targets."},{"key":"ref_41","first-page":"1724","article-title":"Activation of Transient Receptor Potential Vanilloid 1 Protects the Heart against Apoptosis in Ischemia\/Reperfusion Injury through Upregulating the PI3K\/Akt Signaling Pathway","volume":"41","author":"Jiang","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1093\/toxsci\/kfy262","article-title":"Ambient Particulate Matter and Acrolein Co-Exposure Increases Myocardial Dyssynchrony in Mice via TRPA1","volume":"167","author":"Thompson","year":"2019","journal-title":"Toxicol. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.taap.2016.10.008","article-title":"TRPA1 Mediates Changes in Heart Rate Variability and Cardiac Mechanical Function in Mice Exposed to Acrolein","volume":"324","author":"Kurhanewicz","year":"2017","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1152\/japplphysiol.00944.2015","article-title":"Characterization of Cardiovascular Reflexes Evoked by Airway Stimulation with Allylisothiocyanate, Capsaicin, and ATP in Sprague-Dawley Rats","volume":"120","author":"Hooper","year":"2016","journal-title":"J. Appl. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/35012076","article-title":"Vanilloid Receptor-1 Is Essential for Inflammatory Thermal Hyperalgesia","volume":"405","author":"Davis","year":"2000","journal-title":"Nature"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.neuron.2006.03.042","article-title":"TRPA1 Contributes to Cold, Mechanical, and Chemical Nociception but Is Not Essential for Hair-Cell Transduction","volume":"50","author":"Kwan","year":"2006","journal-title":"Neuron"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"15859","DOI":"10.1021\/jacs.5b10162","article-title":"A Potent and Site-Selective Agonist of TRPA1","volume":"137","author":"Takaya","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1016\/j.pain.2011.01.049","article-title":"Selective Blockade of TRPA1 Channel Attenuates Pathological Pain without Altering Noxious Cold Sensation or Body Temperature Regulation","volume":"152","author":"Chen","year":"2011","journal-title":"Pain"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1093\/cvr\/cvac102","article-title":"Methylation of the Hippo Effector YAP by the Methyltransferase SETD7 Drives Myocardial Ischaemic Injury: A Translational Study","volume":"118","author":"Ambrosini","year":"2022","journal-title":"Cardiovasc. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/apha.12861","article-title":"Vagal Nerve Stimulation Reduces Infarct Size via a Mechanism Involving the Alpha-7 Nicotinic Acetylcholine Receptor and Downregulation of Cardiac and Vascular Arginase","volume":"221","author":"Kiss","year":"2017","journal-title":"Acta Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.yjmcc.2011.02.018","article-title":"Retrograde Heart Perfusion: The Langendorff Technique of Isolated Heart Perfusion","volume":"50","author":"Bell","year":"2011","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1161\/CIRCRESAHA.116.309202","article-title":"A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart","volume":"119","author":"Li","year":"2016","journal-title":"Circ. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 Years of Image Analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"H891","DOI":"10.1152\/ajpheart.00259.2019","article-title":"Guidelines for Evaluating Myocardial Cell Death","volume":"317","author":"Mishra","year":"2019","journal-title":"Am. J. Physiol. Heart Circ. Physiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/3\/2516\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:17:49Z","timestamp":1760120269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/3\/2516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,28]]},"references-count":54,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ijms24032516"],"URL":"https:\/\/doi.org\/10.3390\/ijms24032516","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,28]]}}}