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A large number of \u00b7OH, highly toxic to cells, will be produced if too much ROS is not quickly cleared. At the heart of antioxidant enzymes is superoxide dismutase (SOD), which is the first antioxidant enzyme to function in the active oxygen scavenging system. To shield cells from oxidative injury, SOD dismutation superoxide anion free radicals generate hydrogen peroxide and molecule oxygen. Cu\/Zn SOD is a kind of SOD antioxidant enzyme that is mostly found in higher plants\u2019 cytoplasm and chloroplasts. Other studies have demonstrated the significance of the miR398s family of miRNAs in the response of plants to environmental stress. The cleavage location of potato stu-miR398b-3p on Cu\/Zn SOD mRNA was verified using RLM-5\u2032RACE. Using the potato variety \u2018Desiree\u2019, the stu-miR398b-3p overexpression mutant was created, and transgenic lines were raised. SOD activity in transgenic lines was discovered to be decreased during drought stress, although other antioxidant enzyme activities were mostly unaltered. Transgenic plants will wilt more quickly than wild-type plants without irrigation. Additionally, this demonstrates that the response of Cu\/Zn SOD to drought stress is adversely regulated by potato stu-miR398b-3p.<\/jats:p>","DOI":"10.3390\/ijms24032525","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T04:58:25Z","timestamp":1675054705000},"page":"2525","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Potato Stu-miR398b-3p Negatively Regulates Cu\/Zn-SOD Response to Drought Tolerance"],"prefix":"10.3390","volume":"24","author":[{"given":"Zhiyong","family":"Zheng","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangwei","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0768-6554","authenticated-orcid":false,"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7288-2677","authenticated-orcid":false,"given":"Huaijun","family":"Si","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1126\/science.1076311","article-title":"Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA","volume":"297","author":"Llave","year":"2002","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1105\/tpc.105.031682","article-title":"Cloning and characterization of microRNAs from rice","volume":"17","author":"Sunkar","year":"2005","journal-title":"Plant Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1093\/jxb\/erl013","article-title":"Characterization of five microRNA families in maize","volume":"57","author":"Mica","year":"2006","journal-title":"J. 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