{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T13:49:43Z","timestamp":1778939383807,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T00:00:00Z","timestamp":1674864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovak Research and Development Agency","award":["APVV-19-0476"],"award-info":[{"award-number":["APVV-19-0476"]}]},{"name":"Slovak Research and Development Agency","award":["ITMS2014+: 313011V446"],"award-info":[{"award-number":["ITMS2014+: 313011V446"]}]},{"name":"Project of the European Regional Development Fund OP Integrated Structure 2014\u20132020 entitled","award":["APVV-19-0476"],"award-info":[{"award-number":["APVV-19-0476"]}]},{"name":"Project of the European Regional Development Fund OP Integrated Structure 2014\u20132020 entitled","award":["ITMS2014+: 313011V446"],"award-info":[{"award-number":["ITMS2014+: 313011V446"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human papillomavirus (HPV)-associated lesions and malignancies exhibit alterations in the composition and functionality of the extracellular matrix (ECM) that represent the complex molecular pathways present between infection and disease. A total of 20 urine samples were used, including from 10 patients with cervical intraepithelial neoplasia grade 3 (CIN3) and 10 healthy controls to perform the label-free quantitative analysis using the nano-HPLC and ESI-MS ion trap mass analyzer and matrix-assisted laser desorption ionization\u2013time-of-flight mass spectrometry (MALDI-TOF\/MS) fast screening. Among 476 identified\/quantified proteins, 48 were significantly changed (log2-fold change \u22651.0 or \u2264\u22121.0, \u2212log10 (bbinominal, p-value \u2265 1.3), of which were 40 proteins (down-regulated) and 8 proteins (up-regulated) in CIN3, in comparison to healthy controls. The biological function and key pathway enrichment of the gene set using gen set enrichment analysis (GSEA) were analyzed. The ECM-receptor interaction pathway (NES = \u22121.64, p = 0.026) was down-regulated by 13 proteins (HSPG2, COL6A1, COL6A3, SPP1, THBS1, TNC, DAG1, FN1, COMP, GP6, VTN, SDC1, and CD44; log2 FC range from \u22120.03 to \u22121.48) for the CIN3 group in the KEGG database. The MALDI-TOF\/MS screening showed the difference of protein profiles between the control and CIN3 groups, i.e., using the scatter plot with a well-separated shape, as well as effectively distinguishing both groups (control and CIN3) using genetic algorithms (GA) with cross-validation (51.56%) and recognition capability (95.0%). Decreased levels of ECM-receptor interaction proteins may cause disturbances in the interactions of cells with the ECM and play an important role in the development and progression of cervical cancer.<\/jats:p>","DOI":"10.3390\/ijms24032531","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T04:58:25Z","timestamp":1675054705000},"page":"2531","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Differential Urinary Proteomic Analysis of High-Risk Cervical Intraepithelial Neoplasia"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6594-3689","authenticated-orcid":false,"given":"Peter","family":"Bober","sequence":"first","affiliation":[{"name":"Department of Medical and Clinical Biophysics, Faculty of Medicine, University of Pavol Jozef \u0160af\u00e1rik in Ko\u0161ice, Trieda SNP 1, 04011 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So\u0148a","family":"Tk\u00e1\u010dikov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biophysics, Faculty of Medicine, University of Pavol Jozef \u0160af\u00e1rik in Ko\u0161ice, Trieda SNP 1, 04011 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9698-6024","authenticated-orcid":false,"given":"Ivan","family":"Talian","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biophysics, Faculty of Medicine, University of Pavol Jozef \u0160af\u00e1rik in Ko\u0161ice, Trieda SNP 1, 04011 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9718-232X","authenticated-orcid":false,"given":"Peter","family":"Urdz\u00edk","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, Faculty of Medicine, University of Pavol Jozef \u0160af\u00e1rik in Ko\u0161ice, Trieda SNP 1, 04011 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Toporcerov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, Faculty of Medicine, University of Pavol Jozef \u0160af\u00e1rik in Ko\u0161ice, Trieda SNP 1, 04011 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e1n","family":"Sabo","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biophysics, Faculty of Medicine, University of Pavol Jozef \u0160af\u00e1rik in Ko\u0161ice, Trieda SNP 1, 04011 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1136\/jcp.55.4.244","article-title":"The Causal Relation between Human Papillomavirus and Cervical Cancer","volume":"55","author":"Bosch","year":"2002","journal-title":"J. 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