{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T08:39:12Z","timestamp":1778661552738,"version":"3.51.4"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:00:00Z","timestamp":1675123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K08287"],"award-info":[{"award-number":["17K08287"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22H05577"],"award-info":[{"award-number":["22H05577"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hydrogen sulfide (H2S) has been shown to act as both anti-inflammatory and pro-inflammatory mediators. Application of H2S donors generally protects against inflammation; however, experimental results using mice lacking endogenous H2S-producing enzymes, such as cystathionine \u03b3-lyase (CTH) and mercaptopyruvate sulfurtransferase (MPST), are often contradictory. We herein examined two types of model hapten-induced inflammation models, colitis (an inflammatory bowel disease model of mucosal immunity) and contact dermatitis (a type IV allergic model of systemic immunity), in CTH-deficient (Cth\u2013\/\u2013) and MPST-deficient (Mpst\u2013\/\u2013) mice. Both mice exhibited no significant alteration from wild-type mice in trinitrobenzene sulfonic acid (Th1-type hapten)-induced colitis (a Crohn\u2019s disease model) and oxazolone (Th1\/Th2 mix-type; Th2 dominant)-induced colitis (an ulcerative colitis model). However, Cth\u2013\/\u2013 (not Mpst\u2013\/\u2013) mice displayed more exacerbated phenotypes in trinitrochlorobenzene (TNCB; Th1-type)-induced contact dermatitis, but not oxazolone, at the delayed phase (24 h post-administration) of inflammation. CTH mRNA expression was upregulated in the TNCB-treated ears of both wild-type and Mpst\u2013\/\u2013 mice. Although mRNA expression of pro-inflammatory cytokines (IL-1\u03b2 and IL-6) was upregulated in both early (2 h) and delayed phases of TNCB-triggered dermatitis in all genotypes, that of Th2 (IL-4) and Treg cytokines (IL-10) was upregulated only in Cth\u2013\/\u2013 mice, when that of Th1 cytokines (IFN\u03b3 and IL-2) was upregulated in wild-type and Mpst\u2013\/\u2013 mice at the delayed phase. These results suggest that (upregulated) CTH or H2S produced by it helps maintain Th1\/Th2 balance to protect against contact dermatitis.<\/jats:p>","DOI":"10.3390\/ijms24032659","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T01:35:45Z","timestamp":1675128945000},"page":"2659","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Differential Roles of Cystathionine Gamma-Lyase and Mercaptopyruvate Sulfurtransferase in Hapten-Induced Colitis and Contact Dermatitis in Mice"],"prefix":"10.3390","volume":"24","author":[{"given":"Noriyuki","family":"Akahoshi","sequence":"first","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoka","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rintaro","family":"Takemoto","sequence":"additional","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiki","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waka","family":"Kamichatani","sequence":"additional","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5367-205X","authenticated-orcid":false,"given":"Isao","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"M\u2019Koma, A.E. 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