{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:43:03Z","timestamp":1791895383112,"version":"4.3.4"},"reference-count":64,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>One of the most critical issues to be solved in reproductive medicine is the treatment of patients with multiple failures of assisted reproductive treatment caused by low-quality embryos. This study investigated whether mitochondrial transfer to human oocytes improves embryo quality and provides subsequent acceptable clinical results and normality to children born due to the use of this technology. We transferred autologous mitochondria extracted from oogonia stem cells to mature oocytes with sperm at the time of intracytoplasmic sperm injection in 52 patients with recurrent failures (average 5.3 times). We assessed embryo quality using the following three methods: good-quality embryo rates, transferable embryo rates, and a novel embryo-scoring system (embryo quality score; EQS) in 33 patients who meet the preset inclusion criteria for analysis. We also evaluated the clinical outcomes of the in vitro fertilization and development of children born using this technology and compared the mtDNA sequences of the children and their mothers. The good-quality embryo rates, transferable embryo rates, and EQS significantly increased after mitochondrial transfer and resulted in 13 babies born in normal conditions. The mtDNA sequences were almost identical to the respective maternal sequences at the 83 major sites examined. Mitochondrial transfer into human oocytes is an effective clinical option to enhance embryo quality in recurrent in vitro fertilization-failure cases.<\/jats:p>","DOI":"10.3390\/ijms24032738","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T02:52:02Z","timestamp":1675219922000},"page":"2738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Mitochondrial Transfer into Human Oocytes Improved Embryo Quality and Clinical Outcomes in Recurrent Pregnancy Failure Cases"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2834-2726","authenticated-orcid":false,"given":"Yoshiharu","family":"Morimoto","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udayanga Sanath Kankanam","family":"Gamage","sequence":"additional","affiliation":[{"name":"Department of Research, HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Yamochi","sequence":"additional","affiliation":[{"name":"Reproductive Science Institute, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriatsu","family":"Saeki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Nippon Life Hospital, Osaka 550-0006, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoharu","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, IVF Namba Clinic, Osaka 550-0015, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"Department of Research, IVF Namba Clinic, Osaka 550-0015, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Koike","sequence":"additional","affiliation":[{"name":"Department of Reproductive Technology, HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Reproductive Technology, HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumiko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Integrated Medicine, HORAC Grand Front Osaka Clinic, Osaka 530-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisaku","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, IVF Osaka Clinic, Osaka 577-0012, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9083-1403","authenticated-orcid":false,"given":"Shu","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Reproductive Science Institute, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuzo","family":"Yanagimachi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Biochemistry and Physiology, John A. 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