{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T11:26:35Z","timestamp":1784978795864,"version":"3.55.0"},"reference-count":61,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31702310"],"award-info":[{"award-number":["31702310"]}]},{"name":"National Natural Science Foundation of China","award":["2020JM-164"],"award-info":[{"award-number":["2020JM-164"]}]},{"name":"Natural Science Basic Research Plan in Shaanxi Province of China","award":["31702310"],"award-info":[{"award-number":["31702310"]}]},{"name":"Natural Science Basic Research Plan in Shaanxi Province of China","award":["2020JM-164"],"award-info":[{"award-number":["2020JM-164"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Exosomes have the ability to carry a wide range of chemicals, convey them to target cells or target regions, and act as \u201cmessengers.\u201d For the purpose of investigating embryo attachment, it is helpful to comprehend the range of exosomal mRNAs and miRNAs derived from the uterine flushing fluid before and after embryo attachment. In this study, we recovered exosomes from goat uterine rinsing fluid at 5, 15, and 18 days of gestation and used RNA-Seq to identify the mRNA and miRNA profiles of exosomes obtained from uterine rinsing fluid before and after embryo implantation. In total, 91 differently expressed miRNAs and 27,487 differentially expressed mRNAs were found. The target genes predicted by the differentially expressed miRNAs and the differentially expressed mRNAs were mainly membrane-related organelles with catalytic activity, binding activity, transcriptional regulation activity, and involved in metabolism, biological regulation, development, and other processes. This was revealed by GO analysis. Furthermore, KEGG analysis revealed that they were abundant in signaling pathways associated with embryo implantation, including the \u201cPI3K-Akt signaling pathway,\u201d \u201cToll-like receptor signaling pathway,\u201d \u201cTGF-beta signaling route,\u201d \u201cNotch signaling pathway,\u201d and others. Moreover, our research has demonstrated, for the first time, that chi-let-7b-5p specifically targets the 3\u2019UTR of CXCL10. Our research offers a fresh viewpoint on the mechanics of embryo attachment.<\/jats:p>","DOI":"10.3390\/ijms24032799","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T02:21:12Z","timestamp":1675304472000},"page":"2799","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Uterine Flushing Fluid-Derived Let-7b Targets CXCL10 to Regulate Uterine Receptivity in Goats during Embryo Implantation"],"prefix":"10.3390","volume":"24","author":[{"given":"Xinnuan","family":"Ning","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"},{"name":"Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/nrg1808","article-title":"Roadmap to embryo implantation: Clues from mouse models","volume":"7","author":"Wang","year":"2006","journal-title":"Nat. Rev. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.bpobgyn.2011.01.003","article-title":"A guide towards pre-pregnancy management of defective implantation and placentation","volume":"25","author":"Urato","year":"2011","journal-title":"Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.anireprosci.2010.12.001","article-title":"A review of the causes of poor fertility in high milk producing dairy cows","volume":"123","author":"Walsh","year":"2011","journal-title":"Anim. Reprod. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41556-018-0250-9","article-title":"Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication","volume":"21","author":"Mathieu","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.ceb.2009.03.007","article-title":"Exosomes--vesicular carriers for intercellular communication","volume":"21","author":"Simons","year":"2009","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-cellbio-101512-122326","article-title":"Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles","volume":"30","author":"Colombo","year":"2014","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1172\/JCI81135","article-title":"The biology and function of exosomes in cancer","volume":"126","author":"Kalluri","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1210\/jc.2017-00672","article-title":"Placental Exosomes as Early Biomarker of Preeclampsia: Potential Role of Exosomal MicroRNAs Across Gestation","volume":"102","author":"Salomon","year":"2017","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"9355","DOI":"10.2147\/IJN.S281890","article-title":"Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate","volume":"15","author":"Shao","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1002\/pmic.200800109","article-title":"Proteomic profiling of exosomes: Current perspectives","volume":"8","author":"Simpson","year":"2008","journal-title":"Proteomics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.gpb.2015.02.001","article-title":"Exosome and exosomal microRNA: Trafficking, sorting, and function","volume":"13","author":"Zhang","year":"2015","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ng, Y.H., Rome, S., Jalabert, A., Forterre, A., Singh, H., Hincks, C.L., and Salamonsen, L.A. (2013). Endometrial exosomes\/microvesicles in the uterine microenvironment: A new paradigm for embryo-endometrial cross talk at implantation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058502"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Burns, G., Brooks, K., Wildung, M., Navakanitworakul, R., Christenson, L.K., and Spencer, T.E. (2014). Extracellular vesicles in luminal fluid of the ovine uterus. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0090913"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1071\/RD14452","article-title":"Effects of age on follicular fluid exosomal microRNAs and granulosa cell transforming growth factor-\u03b2 signalling during follicle development in the mare","volume":"27","author":"Winger","year":"2015","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1095\/biolreprod.115.134890","article-title":"Human Endometrial Exosomes Contain Hormone-Specific Cargo Modulating Trophoblast Adhesive Capacity: Insights into Endometrial-Embryo Interactions","volume":"94","author":"Greening","year":"2016","journal-title":"Biol. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/nrd.2016.246","article-title":"MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases","volume":"16","author":"Rupaimoole","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1038\/35002607","article-title":"The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans","volume":"403","author":"Reinhart","year":"2000","journal-title":"Nature"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"eaaz7070","DOI":"10.1126\/sciadv.aaz7070","article-title":"Let-7 derived from endometrial extracellular vesicles is an important inducer of embryonic diapause in mice","volume":"6","author":"Liu","year":"2020","journal-title":"Sci. Adv."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1262\/jrd.09-088K","article-title":"Temporal and spatial regulation of let-7a in the uterus during embryo implantation in the rat","volume":"56","author":"Xia","year":"2010","journal-title":"J. Reprod. Dev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1186\/s12964-019-0490-8","article-title":"The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation","volume":"17","author":"Yu","year":"2019","journal-title":"Cell Commun. Signal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3233\/CBM-190483","article-title":"Evaluating prognostic utility of preoperative Neutrophil to Lymphocyte Ratio and hsa-let-7g\/c up-regulation in patients with urinary bladder cancer","volume":"27","author":"Boubaker","year":"2020","journal-title":"Cancer Biomark"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"de Almeida, B.C., Dos Anjos, L.G., Uno, M., Cunha, I.W.D., Soares, F.A., Baiocchi, G., Baracat, E.C., and Carvalho, K.C. (2019). Let-7 miRNA\u2019s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma. Cells, 8.","DOI":"10.3390\/cells8111452"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1101\/pdb.top074476","article-title":"Strategies for isolation of exosomes","volume":"2015","author":"Zeringer","year":"2015","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1089\/cell.2014.0003","article-title":"Improvement of cloned embryos development by co-culturing with parthenotes: A possible role of exosomes\/microvesicles for embryos paracrine communication","volume":"16","author":"Saadeldin","year":"2014","journal-title":"Cell Reprogram."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5515","DOI":"10.4049\/jimmunol.1301885","article-title":"Exosomes secreted by human placenta carry functional Fas ligand and TRAIL molecules and convey apoptosis in activated immune cells, suggesting exosome-mediated immune privilege of the fetus","volume":"191","author":"Stenqvist","year":"2013","journal-title":"J. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4534","DOI":"10.1096\/fj.201701337RR","article-title":"Maternal and umbilical cord serum-derived exosomes enhance endothelial cell proliferation and migration","volume":"32","author":"Jia","year":"2018","journal-title":"Faseb J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1093\/molehr\/gaaa034","article-title":"Exosomes and soluble secretome from hormone-treated endometrial epithelial cells direct embryo implantation","volume":"26","author":"Gurung","year":"2020","journal-title":"Mol. Hum. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.1002\/jcp.30180","article-title":"Exosomes from bovine endometrial epithelial cells ensure trophoblast cell development by miR-218 targeting secreted frizzled related protein 2","volume":"236","author":"Wang","year":"2021","journal-title":"J. Cell Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1007\/s13258-018-0668-2","article-title":"Profiling and identification of pregnancy-associated circulating microRNAs in dairy cattle","volume":"40","author":"Markkandan","year":"2018","journal-title":"Genes Genom."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.2217\/epi-2020-0084","article-title":"Analysis of exosomal lncRNA, miRNA and mRNA expression profiles and ceRNA network construction in endometriosis","volume":"12","author":"Wu","year":"2020","journal-title":"Epigenomics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.1002\/jcp.27203","article-title":"Noncoding RNAs in acute kidney injury","volume":"234","author":"Ren","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e13031","DOI":"10.1111\/adb.13031","article-title":"Circular RNA expression alteration identifies a novel circulating biomarker in serum exosomal for detection of alcohol dependence","volume":"26","author":"Liu","year":"2021","journal-title":"Addict. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1007\/s11596-019-2134-z","article-title":"Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation","volume":"39","author":"Li","year":"2019","journal-title":"Curr. Med. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.ydbio.2005.05.033","article-title":"The PI3K\/Akt pathway is present and functional in the preimplantation mouse embryo","volume":"284","author":"Riley","year":"2005","journal-title":"Dev. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6010","DOI":"10.1074\/jbc.M506982200","article-title":"Phosphatidylinositol 3-kinase activity is critical for glucose metabolism and embryo survival in murine blastocysts","volume":"281","author":"Riley","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.3892\/ijmm.2014.1701","article-title":"The PI3K\/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA","volume":"33","author":"Liu","year":"2014","journal-title":"Int. J. Mol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Ochoa-Bernal, M.A., and Fazleabas, A.T. (2020). Physiologic Events of Embryo Implantation and Decidualization in Human and Non-Human Primates. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21061973"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"447","DOI":"10.7150\/ijms.4593","article-title":"Notch signaling pathway and human placenta","volume":"9","author":"Zhao","year":"2012","journal-title":"Int. J. Med. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1262\/jrd.2014-106","article-title":"Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation","volume":"61","author":"Inyawilert","year":"2015","journal-title":"J. Reprod. Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40104-018-0263-z","article-title":"Gene expression of CCL8 and CXCL10 in peripheral blood leukocytes during early pregnancy in cows","volume":"9","author":"Sakumoto","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/biolre\/iox074","article-title":"Analysis of cysteine-X-cysteine motif chemokine ligands 9, 10, and 11, their receptor CXCR3, and their possible role on the recruitment of immune cells at the maternal-conceptus interface in pigs","volume":"97","author":"Han","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1093\/biolre\/ioac181","article-title":"Expression of microRNA let-7 in cleavage embryos modulates cell fate determination and formation of mouse blastocysts","volume":"107","author":"Liu","year":"2022","journal-title":"Biol. Reprod."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"843","DOI":"10.4161\/cc.8.6.7907","article-title":"Let-7 and miR-200 microRNAs: Guardians against pluripotency and cancer progression","volume":"8","author":"Peter","year":"2009","journal-title":"Cell Cycle"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4344","DOI":"10.4049\/jimmunol.1601070","article-title":"Lin28b Regulates Fetal Regulatory T Cell Differentiation through Modulation of TGF-\u03b2 Signaling","volume":"197","author":"Bronevetsky","year":"2016","journal-title":"J. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1093\/biolre\/ioaa132","article-title":"Pig conceptuses secrete interferon gamma to recruit T cells to the endometrium during the peri-implantation period","volume":"103","author":"McLendon","year":"2020","journal-title":"Biol. Reprod."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Th\u00e9ry, C., Amigorena, S., Raposo, G., and Clayton, A. (2006). Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol., Chapter 3, Unit 3.22.","DOI":"10.1002\/0471143030.cb0322s30"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.cell.2019.02.029","article-title":"Reassessment of Exosome Composition","volume":"177","author":"Jeppesen","year":"2019","journal-title":"Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ymeth.2015.05.028","article-title":"Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches","volume":"87","author":"Zarovni","year":"2015","journal-title":"Methods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"F1657","DOI":"10.1152\/ajprenal.00434.2006","article-title":"Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator","volume":"292","author":"Cheruvanky","year":"2007","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1479-5876-9-47","article-title":"Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs","volume":"9","author":"Chen","year":"2011","journal-title":"J. Transl. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Li, B., and Dewey, C.N. (2011). RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btp612","article-title":"DEGseq: An R package for identifying differentially expressed genes from RNA-seq data","volume":"26","author":"Wang","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1101\/gr.7.10.986","article-title":"The significance of digital gene expression profiles","volume":"7","author":"Audic","year":"1997","journal-title":"Genome Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"W451","DOI":"10.1093\/nar\/gkl243","article-title":"RNAhybrid: MicroRNA target prediction easy, fast and flexible","volume":"34","author":"Rehmsmeier","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"John, B., Enright, A.J., Aravin, A., Tuschl, T., Sander, C., and Marks, D.S. (2004). Human MicroRNA targets. PLoS Biol., 2.","DOI":"10.1371\/journal.pbio.0020363"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e05005","DOI":"10.7554\/eLife.05005","article-title":"Predicting effective microRNA target sites in mammalian mRNAs","volume":"4","author":"Agarwal","year":"2015","journal-title":"Elife"},{"key":"ref_59","unstructured":"Abdi, H. (2007). Encyclopedia of Measurement and Statistics, Sage."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"D480","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for linking genomes to life and the environment","volume":"36","author":"Kanehisa","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.3923\/javaa.2010.2738.2747","article-title":"Establishment and characteristics of immortal goat endometrial epithelial cells and stromal cells with hTERT","volume":"9","author":"Zhang","year":"2010","journal-title":"J. Anim. Vet. Adv."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/3\/2799\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:21:15Z","timestamp":1760120475000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/3\/2799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,1]]},"references-count":61,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ijms24032799"],"URL":"https:\/\/doi.org\/10.3390\/ijms24032799","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,1]]}}}