{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:34:59Z","timestamp":1791920099765,"version":"4.3.4"},"reference-count":100,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ram\u00f3n Areces Foundation","award":["(12\/2014)"],"award-info":[{"award-number":["(12\/2014)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Iron overload caused by hereditary hemochromatosis (HH) increases free reactive oxygen species that, in turn, induce lipid peroxidation. Its 4-hydroxynonenal (HNE) by-product is a well-established marker of lipid peroxidation since it reacts with accessible proteins with deleterious consequences. Indeed, elevated levels of HNE are often detected in a wide variety of human diseases related to oxidative stress. Here, we evaluated HNE-modified proteins in the membrane of erythrocytes from HH patients and in organs of Hfe\u2212\/\u2212 male and female mice, a mouse model of HH. For this purpose, we used one- and two-dimensional gel electrophoresis, immunoblotting and MALDI-TOF\/TOF analysis. We identified cytoskeletal membrane proteins and membrane receptors of erythrocytes bound to HNE exclusively in HH patients. Furthermore, kidney and brain of Hfe\u2212\/\u2212 mice contained more HNE-adducted protein than healthy controls. Our results identified main HNE-modified proteins suggesting that HH favours preferred protein targets for oxidation by HNE.<\/jats:p>","DOI":"10.3390\/ijms24032922","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T02:35:10Z","timestamp":1675391710000},"page":"2922","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9369-6139","authenticated-orcid":false,"given":"Sandra","family":"S\u00e1nchez-Jaut","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"P\u00e9rez-Benavente","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Abad","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3905-6859","authenticated-orcid":false,"given":"Dar\u00edo","family":"M\u00e9ndez-Cuadro","sequence":"additional","affiliation":[{"name":"Analytical Chemistry and Biomedicine Group, School of Exact and Natural Sciences, San Pablo Campus, University of Cartagena, Cartagena 130014, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Puyet","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amalia","family":"Diez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Galicia-Poblet","sequence":"additional","affiliation":[{"name":"Pediatric Digestive Service, Guadalajara University Hospital, 19002 Guadalajara, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0072-459X","authenticated-orcid":false,"given":"Elena","family":"G\u00f3mez-Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Moran-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-881X","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Bautista","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4545-1254","authenticated-orcid":false,"given":"Isabel G.","family":"Azc\u00e1rate","sequence":"additional","affiliation":[{"name":"Department of Medical Specialties and Public Health, Rey Juan Carlos University, 28922 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/ng1274","article-title":"Mutations in HFE2 cause iron overload in chromosome 1q\u2013linked juvenile hemochromatosis","volume":"36","author":"Papanikolaou","year":"2004","journal-title":"Nat. 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