{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:02:37Z","timestamp":1792036957135,"version":"4.4.0"},"reference-count":21,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-15-00140"],"award-info":[{"award-number":["21-15-00140"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Integrins are cell adhesion receptors, which play a role in breast cancer invasion, angiogenesis, and metastasis. Moreover, it has been shown that exosomal integrins provide organotropic metastasis in a mouse model. In our study, we aimed to investigate the expression of integrins \u03b23, \u03b24, and \u03b1V\u03b25 on exosomes and tumor cells (circulating tumor cells and primary tumor) and their association with the localization of distant metastasis. We confirmed the association of exosomal integrin \u03b24 with lung metastasis in breast cancer patients. However, we were unable to evaluate the role of integrin \u03b23 in brain metastasis due to the rarity of this localization. We established no association of exosomal integrin \u03b1V\u03b25 with liver metastasis in our cohort of breast cancer patients. The further evaluation of \u03b23, \u03b24, and \u03b1V\u03b25 integrin expression on CTCs revealed an association of integrin \u03b24 and \u03b1V\u03b25 with liver, but not the lung metastases. Integrin \u03b24 in the primary tumor was associated with liver metastasis. Furthermore, an in-depth analysis of phenotypic characteristics of \u03b24+ tumor cells revealed a significantly increased proportion of E-cadherin+ and CD44+CD24- cells in patients with liver metastases compared to patients with lung or no distant metastases.<\/jats:p>","DOI":"10.3390\/ijms24032929","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T02:35:10Z","timestamp":1675391710000},"page":"2929","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["The Association of Integrins \u03b23, \u03b24, and \u03b1V\u03b25 on Exosomes, CTCs and Tumor Cells with Localization of Distant Metastasis in Breast Cancer Patients"],"prefix":"10.3390","volume":"24","author":[{"given":"Evgeniya S.","family":"Grigoryeva","sequence":"first","affiliation":[{"name":"The Laboratory of Molecular Therapy of Cancer, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"},{"name":"The Laboratory of Molecular Oncology and Immunology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2061-8417","authenticated-orcid":false,"given":"Luibov A.","family":"Tashireva","sequence":"additional","affiliation":[{"name":"The Laboratory of Molecular Therapy of Cancer, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"},{"name":"The Department of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0301-8455","authenticated-orcid":false,"given":"Olga E.","family":"Savelieva","sequence":"additional","affiliation":[{"name":"The Department of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"},{"name":"Research Center, Saint-Petersburg State Pediatric Medical University, 194100 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V.","family":"Zavyalova","sequence":"additional","affiliation":[{"name":"The Department of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliya O.","family":"Popova","sequence":"additional","affiliation":[{"name":"The Department of Chemotherapy, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9443-7224","authenticated-orcid":false,"given":"Gleb A.","family":"Kuznetsov","sequence":"additional","affiliation":[{"name":"The Department of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena S.","family":"Andryuhova","sequence":"additional","affiliation":[{"name":"The Department of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir M.","family":"Perelmuter","sequence":"additional","affiliation":[{"name":"The Department of General and Molecular Pathology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s13046-018-0763-x","article-title":"Integrins as therapeutic targets in the organ-specific metastasis of human malignant melanoma","volume":"37","author":"Huang","year":"2018","journal-title":"J. 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