{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:51:19Z","timestamp":1790783479637,"version":"4.1.0"},"reference-count":31,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T00:00:00Z","timestamp":1675382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness, co-financed by the Carlos III Health Institute (ISCIII) and European Regional Development Fund (FEDER)","award":["PI19\/01096"],"award-info":[{"award-number":["PI19\/01096"]}]},{"name":"Spanish Ministry of Economy and Competitiveness, co-financed by the Carlos III Health Institute (ISCIII) and European Regional Development Fund (FEDER)","award":["P20_00646"],"award-info":[{"award-number":["P20_00646"]}]},{"name":"Spanish Ministry of Economy and Competitiveness, co-financed by the Carlos III Health Institute (ISCIII) and European Regional Development Fund (FEDER)","award":["PAIDI-2020"],"award-info":[{"award-number":["PAIDI-2020"]}]},{"name":"Spanish Ministry of Economy and Competitiveness, co-financed by the Carlos III Health Institute (ISCIII) and European Regional Development Fund (FEDER)","award":["FPU17\/04825"],"award-info":[{"award-number":["FPU17\/04825"]}]},{"name":"Regional Department of Economy, Knowledge, Business and University","award":["PI19\/01096"],"award-info":[{"award-number":["PI19\/01096"]}]},{"name":"Regional Department of Economy, Knowledge, Business and University","award":["P20_00646"],"award-info":[{"award-number":["P20_00646"]}]},{"name":"Regional Department of Economy, Knowledge, Business and University","award":["PAIDI-2020"],"award-info":[{"award-number":["PAIDI-2020"]}]},{"name":"Regional Department of Economy, Knowledge, Business and University","award":["FPU17\/04825"],"award-info":[{"award-number":["FPU17\/04825"]}]},{"name":"Junta de Andaluc\u00eda, Andalusian Plan for Research","award":["PI19\/01096"],"award-info":[{"award-number":["PI19\/01096"]}]},{"name":"Junta de Andaluc\u00eda, Andalusian Plan for Research","award":["P20_00646"],"award-info":[{"award-number":["P20_00646"]}]},{"name":"Junta de Andaluc\u00eda, Andalusian Plan for Research","award":["PAIDI-2020"],"award-info":[{"award-number":["PAIDI-2020"]}]},{"name":"Junta de Andaluc\u00eda, Andalusian Plan for Research","award":["FPU17\/04825"],"award-info":[{"award-number":["FPU17\/04825"]}]},{"name":"Spanish Ministry of Universities","award":["PI19\/01096"],"award-info":[{"award-number":["PI19\/01096"]}]},{"name":"Spanish Ministry of Universities","award":["P20_00646"],"award-info":[{"award-number":["P20_00646"]}]},{"name":"Spanish Ministry of Universities","award":["PAIDI-2020"],"award-info":[{"award-number":["PAIDI-2020"]}]},{"name":"Spanish Ministry of Universities","award":["FPU17\/04825"],"award-info":[{"award-number":["FPU17\/04825"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Aquaporin-4 (AQP4) is the most abundant water channel in the central nervous system and plays a fundamental role in maintaining water homeostasis there. In adult mice, AQP4 is located mainly in ependymal cells, in the endfeet of perivascular astrocytes, and in the glia limitans. Meanwhile, its expression, location, and function throughout postnatal development remain largely unknown. Here, the expression of AQP4 mRNA was studied by in situ hybridization and RT-qPCR, and the localization and amount of protein was studied by immunofluorescence and western blotting, both in the brain and spinal cord. For this, wild-type mice of the C57BL\/6 line, aged 1, 3, 7, 11, 20, and 60 days, and 18 months were used. The results showed a change in both the expression and location of AQP4 in postnatal development compared to those during adult life. In the early stages of postnatal development it appears in highly myelinated areas, such as the corpus callosum or cerebellum, and as the animal grows, it disappears from these areas, passing through the cortical regions of the forebrain and concentrating around the blood vessels. These findings suggest an unprecedented possible role for AQP4 in the early cell differentiation process, during the first days of life in the newborn animal, which will lead to myelination.<\/jats:p>","DOI":"10.3390\/ijms24033048","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T02:57:32Z","timestamp":1675652252000},"page":"3048","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System"],"prefix":"10.3390","volume":"24","author":[{"given":"Francisco","family":"Mayo","sequence":"first","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"},{"name":"Department of Physiology and Biophysics, University of Seville, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Gonz\u00e1lez-Vinceiro","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"},{"name":"Department of Physiology and Biophysics, University of Seville, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Hiraldo-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"},{"name":"Department of Physiology and Biophysics, University of Seville, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Calle-Castillejo","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1324-2872","authenticated-orcid":false,"given":"Sara","family":"Morales-Alvarez","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1594-0404","authenticated-orcid":false,"given":"Reposo","family":"Ram\u00edrez-Lorca","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"},{"name":"Department of Physiology and Biophysics, University of Seville, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4758-9428","authenticated-orcid":false,"given":"Miriam","family":"Echevarr\u00eda","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), Virgen del Roc\u00edo University Hospital, (HUVR)\/Spanish National Research Council (CSIC)\/University of Seville, 41013 Seville, Spain"},{"name":"Department of Physiology and Biophysics, University of Seville, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1097\/00004647-200204000-00001","article-title":"Aquaporins in brain: Distribution, physiology, and pathophysiology","volume":"22","author":"Badaut","year":"2002","journal-title":"J. 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