{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:08:14Z","timestamp":1778832494758,"version":"3.51.4"},"reference-count":42,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Incheon National University","award":["2022R1A4A5034121"],"award-info":[{"award-number":["2022R1A4A5034121"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2022R1A4A5034121"],"award-info":[{"award-number":["2022R1A4A5034121"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Molecular profiling of the hypothalamus in response to metabolic shifts is a critical cue to better understand the principle of the central control of whole-body energy metabolism. The transcriptional responses of the rodent hypothalamus to short-term calorie restriction have been documented. However, studies on the identification of hypothalamic secretory factors that potentially contribute to the control of appetite are lacking. In this study, we analyzed the differential expression of hypothalamic genes and compared the selected secretory factors from the fasted mice with those of fed control mice using bulk RNA-sequencing. We verified seven secretory genes that were significantly altered in the hypothalamus of fasted mice. In addition, we determined the response of secretory genes in cultured hypothalamic cells to treatment with ghrelin and leptin. The current study provides further insights into the neuronal response to food restriction at the molecular level and may be useful for understanding the hypothalamic control of appetite.<\/jats:p>","DOI":"10.3390\/ijms24043204","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T04:59:10Z","timestamp":1675659550000},"page":"3204","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Whole Transcriptome Analysis of Hypothalamus in Mice during Short-Term Starvation"],"prefix":"10.3390","volume":"24","author":[{"given":"Eun-Young","family":"Oh","sequence":"first","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6463-9645","authenticated-orcid":false,"given":"Byong Seo","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Rim","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho Gyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thai Hien","family":"Tu","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunggu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nano-Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Ryung","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Geun","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.cmet.2011.08.009","article-title":"Distinct Hypothalamic Neurons Mediate Estrogenic Effects on Energy Homeostasis and Reproduction","volume":"14","author":"Xu","year":"2011","journal-title":"Cell Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1210\/me.2016-1026","article-title":"Optogenetic Stimulation of Arcuate Nucleus Kiss1 Neurons Reveals a Steroid-Dependent Glutamatergic Input to POMC and AgRP Neurons in Male Mice","volume":"30","author":"Nestor","year":"2016","journal-title":"Mol. 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