{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T15:05:01Z","timestamp":1789830301893,"version":"4.0.1"},"reference-count":54,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council, Taiwan","award":["111-2221-E-182-004-MY3"],"award-info":[{"award-number":["111-2221-E-182-004-MY3"]}]},{"name":"National Science and Technology Council, Taiwan","award":["CMRPD2M0131"],"award-info":[{"award-number":["CMRPD2M0131"]}]},{"name":"National Science and Technology Council, Taiwan","award":["CRRPG3K0081"],"award-info":[{"award-number":["CRRPG3K0081"]}]},{"name":"National Science and Technology Council, Taiwan","award":["CRRPG3K0082"],"award-info":[{"award-number":["CRRPG3K0082"]}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital-Linkou","doi-asserted-by":"publisher","award":["111-2221-E-182-004-MY3"],"award-info":[{"award-number":["111-2221-E-182-004-MY3"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital-Linkou","doi-asserted-by":"publisher","award":["CMRPD2M0131"],"award-info":[{"award-number":["CMRPD2M0131"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital-Linkou","doi-asserted-by":"publisher","award":["CRRPG3K0081"],"award-info":[{"award-number":["CRRPG3K0081"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital-Linkou","doi-asserted-by":"publisher","award":["CRRPG3K0082"],"award-info":[{"award-number":["CRRPG3K0082"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aimed to develop a drug delivery system with hybrid biodegradable antifungal and antibacterial agents incorporated into poly lactic-co-glycolic acid (PLGA) nanofibers, facilitating an extended release of fluconazole, vancomycin, and ceftazidime to treat polymicrobial osteomyelitis. The nanofibers were assessed using scanning electron microscopy, tensile testing, water contact angle analysis, differential scanning calorimetry, and Fourier-transform infrared spectroscopy. The in vitro release of the antimicrobial agents was assessed using an elution method and a high-performance liquid chromatography assay. The in vivo elution pattern of nanofibrous mats was assessed using a rat femoral model. The experimental results demonstrated that the antimicrobial agent-loaded nanofibers released high levels of fluconazole, vancomycin, and ceftazidime for 30 and 56 days in vitro and in vivo, respectively. Histological assays revealed no notable tissue inflammation. Therefore, hybrid biodegradable PLGA nanofibers with a sustainable release of antifungal and antibacterial agents may be employed for the treatment of polymicrobial osteomyelitis.<\/jats:p>","DOI":"10.3390\/ijms24043254","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T02:56:08Z","timestamp":1675738568000},"page":"3254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Sustained Release of Antifungal and Antibacterial Agents from Novel Hybrid Degradable Nanofibers for the Treatment of Polymicrobial Osteomyelitis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7391-3212","authenticated-orcid":false,"given":"Yung-Heng","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital, Tao-Yuan 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Hsun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital, Tao-Yuan 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Chao","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital, Tao-Yuan 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Jung","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Chang Gung University, Tao-Yuan 33302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Chang Gung University, Tao-Yuan 33302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve Wen-Neng","family":"Ueng","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital, Tao-Yuan 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2083-4865","authenticated-orcid":false,"given":"Shih-Jung","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital, Tao-Yuan 33305, Taiwan"},{"name":"Department of Mechanical Engineering, Chang Gung University, Tao-Yuan 33302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1186\/s13018-019-1388-2","article-title":"A systematic review of the single-stage treatment of chronic osteomyelitis","volume":"14","author":"Pincher","year":"2019","journal-title":"J. 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