{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:50:53Z","timestamp":1790247053994,"version":"4.1.0"},"reference-count":69,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T00:00:00Z","timestamp":1675987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United Kingdom Biotechnology and Biological Sciences Research Council","award":["BB\/M025349\/1 (AJQ)"],"award-info":[{"award-number":["BB\/M025349\/1 (AJQ)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The cornea forms the tough and transparent anterior part of the eye and by accurate shaping forms the major refractive element for vision. Its largest component is the stroma, a dense collagenous connective tissue positioned between the epithelium and the endothelium. In chicken embryos, the stroma initially develops as the primary stroma secreted by the epithelium, which is then invaded by migratory neural crest cells. These cells secrete an organised multi-lamellar collagenous extracellular matrix (ECM), becoming keratocytes. Within individual lamellae, collagen fibrils are parallel and orientated approximately orthogonally in adjacent lamellae. In addition to collagens and associated small proteoglycans, the ECM contains the multifunctional adhesive glycoproteins fibronectin and tenascin-C. We show in embryonic chicken corneas that fibronectin is present but is essentially unstructured in the primary stroma before cell migration and develops as strands linking migrating cells as they enter, maintaining their relative positions as they populate the stroma. Fibronectin also becomes prominent in the epithelial basement membrane, from which fibronectin strings penetrate into the stromal lamellar ECM at right angles. These are present throughout embryonic development but are absent in adults. Stromal cells associate with the strings. Since the epithelial basement membrane is the anterior stromal boundary, strings may be used by stromal cells to determine their relative anterior\u2013posterior positions. Tenascin-C is organised differently, initially as an amorphous layer above the endothelium and subsequently extending anteriorly and organising into a 3D mesh when the stromal cells arrive, enclosing them. It continues to shift anteriorly in development, disappearing posteriorly, and finally becoming prominent in Bowman\u2019s layer beneath the epithelium. The similarity of tenascin-C and collagen organisation suggests that it may link cells to collagen, allowing cells to control and organise the developing ECM architecture. Fibronectin and tenascin-C have complementary roles in cell migration, with the former being adhesive and the latter being antiadhesive and able to displace cells from their adhesion to fibronectin. Thus, in addition to the potential for associations between cells and the ECM, the two could be involved in controlling migration and adhesion and subsequent keratocyte differentiation. Despite the similarities in structure and binding capabilities of the two glycoproteins and the fact that they occupy similar regions of the developing stroma, there is little colocalisation, demonstrating their distinctive roles.<\/jats:p>","DOI":"10.3390\/ijms24043555","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T02:56:13Z","timestamp":1675997773000},"page":"3555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Developmental Changes in Patterns of Distribution of Fibronectin and Tenascin-C in the Chicken Cornea: Evidence for Distinct and Independent Functions during Corneal Development and Morphogenesis"],"prefix":"10.3390","volume":"24","author":[{"given":"Elena","family":"Koudouna","sequence":"first","affiliation":[{"name":"Structural Biophysics Group, School of Optometry & Vision Sciences, Cardiff University, Maindy Road, Cathays, Cardiff CF24 4HQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Young","sequence":"additional","affiliation":[{"name":"Structural Biophysics Group, School of Optometry & Vision Sciences, Cardiff University, Maindy Road, Cathays, Cardiff CF24 4HQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Quantock","sequence":"additional","affiliation":[{"name":"Structural Biophysics Group, School of Optometry & Vision Sciences, Cardiff University, Maindy Road, Cathays, Cardiff CF24 4HQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Ralphs","sequence":"additional","affiliation":[{"name":"School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.preteyeres.2018.01.002","article-title":"Evolution of the vertebrate corneal stroma","volume":"64","author":"Koudouna","year":"2018","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5159","DOI":"10.1167\/iovs.09-3669","article-title":"3D collagen orientation study of the human cornea using X-ray diffraction and femtosecond laser technology","volume":"50","author":"Abahussin","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_3","first-page":"1","article-title":"Fine structure of the developing avian cornea","volume":"1","author":"Hay","year":"1969","journal-title":"Monogr. Dev. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1083\/jcb.50.3.840","article-title":"Morphogenesis of the collagenous stroma in the chick cornea","volume":"50","author":"Trelstad","year":"1971","journal-title":"J. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1083\/jcb.67.2.400","article-title":"The behavior of fibroblasts from the developing avian cornea. Morphology and movement in situ and in vitro","volume":"67","author":"Bard","year":"1975","journal-title":"J. Cell Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1083\/jcb.45.1.34","article-title":"The Golgi apparatus in chick corneal epithelium: Changes in intracellular position during development","volume":"45","author":"Trelstad","year":"1970","journal-title":"J. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/0092-8674(80)90526-7","article-title":"Fibroblast adhesion to fibrinogen and fibrin substrata: Requirement for cold-insoluble globulin (plasma fibronectin)","volume":"19","author":"Grinnell","year":"1980","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1177\/002215549704500205","article-title":"Upregulation of alpha 9 integrin and tenascin during epithelial regeneration after debridement in the cornea","volume":"45","author":"Stepp","year":"1997","journal-title":"J. Histochem. Cytochem."},{"key":"ref_9","first-page":"1135","article-title":"Expression of tenascin-C splice variants in normal and bullous keratopathy human corneas","volume":"39","author":"Ljubimov","year":"1998","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1097\/00003226-199805000-00014","article-title":"Novel splice variants of human tenascin-C mRNA identified in normal and bullous keratopathy corneas","volume":"17","author":"Saghizadeh","year":"1998","journal-title":"Cornea."},{"key":"ref_11","first-page":"3703","article-title":"Fibronectin and stem cell differentiation-lessons from chondrogenesis","volume":"125","author":"Singh","year":"2012","journal-title":"J Cell Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.1038\/s41598-017-03701-0","article-title":"Fibronectin promotes directional persistence in fibroblast migration through interactions with both its cell-binding and heparin-binding domains","volume":"7","author":"Missirlis","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1016\/j.yexcr.2010.05.020","article-title":"Syndecan-1 regulates cell migration and fibronectin fibril assembly","volume":"316","author":"Stepp","year":"2010","journal-title":"Exp. Cell Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1620\/tjem.222.45","article-title":"Connective tissue growth factor cooperates with fibronectin in enhancing attachment and migration of corneal epithelial cells","volume":"222","author":"Sugioka","year":"2010","journal-title":"Tohoku J. Exp. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev-cellbio-100109-104020","article-title":"Assembly of fibronectin extracellular matrix","volume":"26","author":"Singh","year":"2010","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1083\/jcb.91.3.673","article-title":"Fibronectin molecule visualized in electron microscopy: A long, thin, flexible strand","volume":"91","author":"Erickson","year":"1981","journal-title":"J. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5146","DOI":"10.1021\/bi992770x","article-title":"Statistical conformation of human plasma fibronectin","volume":"39","author":"Pelta","year":"2000","journal-title":"Biochemistry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3045","DOI":"10.1016\/S0021-9258(18)49952-8","article-title":"Role of type III homology repeats in cell adhesive function within the cell-binding domain of fibronectin","volume":"266","author":"Kimizuka","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/S0006-3495(03)74940-6","article-title":"Force measurements of the alpha5beta1 integrin-fibronectin interaction","volume":"84","author":"Li","year":"2003","journal-title":"Biophys. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1073\/pnas.96.4.1351","article-title":"Forced unfolding of the fibronectin type III module reveals a tensile molecular recognition switch","volume":"96","author":"Krammer","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"18267","DOI":"10.1073\/pnas.0907518106","article-title":"Fibronectin forms the most extensible biological fibers displaying switchable force-exposed cryptic binding sites","volume":"106","author":"Klotzsch","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0092-8674(86)90374-0","article-title":"Tenascin: An extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis","volume":"47","author":"Mackie","year":"1986","journal-title":"Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s12079-009-0075-1","article-title":"The role of tenascin-C in tissue injury and tumorigenesis","volume":"3","author":"Midwood","year":"2009","journal-title":"J. Cell Commun. Signal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.biocel.2015.06.003","article-title":"Tenascin-C: Its functions as an integrin ligand","volume":"65","author":"Tucker","year":"2015","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1083\/jcb.200103103","article-title":"Epidermal growth factor (EGF)-like repeats of human tenascin-C as ligands for EGF receptor","volume":"154","author":"Swindle","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1161\/01.ATV.0000134299.89599.53","article-title":"EGF-Like domain of tenascin-C is proapoptotic for cultured smooth muscle cells","volume":"24","author":"Wallner","year":"2004","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4449","DOI":"10.7150\/jca.25926","article-title":"Tenascin-C modulates cell cycle progression to enhance tumour cell proliferation through AKT\/FOXO1 signalling in pancreatic cancer","volume":"9","author":"Cai","year":"2018","journal-title":"J. Cancer."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1038\/nm.1987","article-title":"Tenascin-C is an endogenous activator of Toll-like receptor 4 that is essential for maintaining inflammation in arthritic joint disease","volume":"15","author":"Midwood","year":"2009","journal-title":"Nat. Med."},{"key":"ref_29","first-page":"8586","article-title":"Interference of tenascin-C with syndecan-4 binding to fibronectin blocks cell adhesion and stimulates tumor cell proliferation","volume":"61","author":"Huang","year":"2001","journal-title":"Cancer Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1242\/jcs.110.7.861","article-title":"Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix","volume":"100","author":"Greiling","year":"1997","journal-title":"J. Cell Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.matbio.2017.06.001","article-title":"Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices","volume":"64","author":"Graham","year":"2017","journal-title":"Matrix Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1242\/dev.112.2.605","article-title":"Tenascin Mr 220,000 isoform expression correlates with corneal cell migration","volume":"112","author":"Kaplony","year":"1991","journal-title":"Development"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1432-0436.1991.tb00243.x","article-title":"The distribution of J1\/tenascin and its transcript during the development of the avian cornea","volume":"48","author":"Tucker","year":"1991","journal-title":"Differentiation"},{"key":"ref_34","first-page":"4101","article-title":"Developmental changes in patterns of expression of tenascin-C variants in the human cornea","volume":"41","author":"Maseruka","year":"2000","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0012-1606(91)90430-B","article-title":"Stromal assemblies containing collagen types IV and VI and fibronectin in the developing embryonic avian cornea","volume":"144","author":"Fitch","year":"1991","journal-title":"Dev. Biol."},{"key":"ref_36","first-page":"231","article-title":"Development and roles of collagenous matrices in the embryonic avian cornea","volume":"17","author":"Linsenmayer","year":"1998","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0014-4835(79)90164-7","article-title":"Origins of avian ocular and periocular tissues","volume":"29","author":"Johnston","year":"1979","journal-title":"Exp. Eye Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/0092-8674(88)90158-4","article-title":"Tenascin interferes with fibronectin action","volume":"53","author":"Kalla","year":"1988","journal-title":"Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/jnr.490210206","article-title":"Distribution and function of tenascin during cranial neural crest development in the chick","volume":"21","year":"1988","journal-title":"J. Neurosci. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1242\/dev.102.1.237","article-title":"The distribution of tenascin coincides with pathways of neural crest cell migration","volume":"102","author":"Mackie","year":"1988","journal-title":"Development"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/dvdy.1171","article-title":"Abnormal neural crest cell migration after the in vivo knockdown of tenascin-C expression with morpholino antisense oligonucleotides","volume":"222","author":"Tucker","year":"2001","journal-title":"Dev. Dyn."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.actbio.2018.08.017","article-title":"Cell regulation of collagen fibril macrostructure during corneal morphogenesis","volume":"79","author":"Koudouna","year":"2018","journal-title":"Acta Biomater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1242\/dev.112.4.1031","article-title":"The expression of tenascin by neural crest cells and glia","volume":"112","author":"Tucker","year":"1991","journal-title":"Development"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1242\/dev.116.1.147","article-title":"Expression of tenascin mRNA in mesoderm during Xenopus laevis embryogenesis: The potential role of mesoderm patterning in tenascin regionalization","volume":"116","author":"Umbhauer","year":"1992","journal-title":"Development"},{"key":"ref_45","first-page":"432","article-title":"Fibroblasts retain their tissue phenotype when grown in three-dimensional collagen gels","volume":"195","author":"Doane","year":"1991","journal-title":"Exp. Eye Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1073\/pnas.1313561110","article-title":"Three-dimensional aspects of matrix assembly by cells in the developing cornea","volume":"111","author":"Young","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/30270","article-title":"The molecular elasticity of the extracellular matrix protein tenascin","volume":"393","author":"Oberhauser","year":"1998","journal-title":"Nature"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1136\/bjo.81.8.677","article-title":"Tenascin-C expression in normal, inflamed, and scarred human corneas","volume":"81","author":"Maseruka","year":"1997","journal-title":"Br. J. Ophthalmol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/jez.1401980209","article-title":"DNA synthesis during the development of the chick cornea","volume":"198","author":"Nuttall","year":"1976","journal-title":"J. Exp. Zool."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1038\/s41467-018-05274-6","article-title":"A positive feedback loop bi-stably activates fibroblasts","volume":"9","author":"Yeo","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"34304","DOI":"10.1074\/jbc.M113.504399","article-title":"The Ets transcription factor EHF as a regulator of cornea epithelial cell identity","volume":"288","author":"Stephens","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ydbio.2011.12.041","article-title":"Neural crest delamination and migration: From epithelium-to-mesenchyme transition to collective cell migration","volume":"366","author":"Theveneau","year":"2012","journal-title":"Dev. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1083\/jcb.95.2.641","article-title":"Domain and basement membrane specificity of a monoclonal antibody against chicken type IV collagen","volume":"95","author":"Fitch","year":"1982","journal-title":"J. Cell Biol."},{"key":"ref_54","first-page":"461","article-title":"Human corneal basement membrane heterogeneity: Topographical differences in the expression of type IV collagen and laminin isoforms","volume":"72","author":"Ljubimov","year":"1995","journal-title":"Lab Investig."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.matbio.2009.02.004","article-title":"Developmental distribution of collagen IV isoforms and relevance to ocular diseases","volume":"28","author":"Bai","year":"2009","journal-title":"Matrix Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"21612","DOI":"10.1074\/jbc.270.37.21612","article-title":"Requirement for the synergy site for cell adhesion to fibronectin depends on the activation state of integrin \u03b15 \u03b21","volume":"270","author":"Danen","year":"1995","journal-title":"J. Biol. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/0092-8674(95)90184-1","article-title":"Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix","volume":"83","author":"Wu","year":"1995","journal-title":"Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1083\/jcb.148.5.1075","article-title":"Integrin dynamics and matrix assembly: Tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis","volume":"148","author":"Pankov","year":"2000","journal-title":"J. Cell Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"20937","DOI":"10.1074\/jbc.M801763200","article-title":"Fibronectin-tissue transglutaminase matrix rescues RGD-impaired cell adhesion through syndecan-4 and beta1 integrin co-signaling","volume":"283","author":"Telci","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1242\/jcs.00357","article-title":"Direct correlation of collagen matrix deformation with focal adhesion dynamics in living corneal fibroblasts","volume":"116","author":"Petroll","year":"2003","journal-title":"J. Cell Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1242\/jcs.00845","article-title":"FAK promotes organization of fibronectin matrix and fibrillar adhesions","volume":"117","author":"Kovacic","year":"2004","journal-title":"J. Cell Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1038\/sj.emboj.7601694","article-title":"Interdomain association in fibronectin: Insight into cryptic sites and fibrillogenesis","volume":"26","author":"Vakonakis","year":"2007","journal-title":"EMBO J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"18061","DOI":"10.1038\/s41598-017-18328-4","article-title":"Multiple Cryptic Binding Sites are Necessary for Robust Fibronectin Assembly: An In Silico Study","volume":"7","author":"Lemmon","year":"2017","journal-title":"Sci Rep."},{"key":"ref_64","first-page":"549","article-title":"Healing corneas express embryonic fibronectin isoforms in the epithelium, subepithelial stroma, and endothelium","volume":"149","author":"Nickeleit","year":"1996","journal-title":"Am. J. Pathol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"489","DOI":"10.3109\/02713688408997237","article-title":"Fibronectin in developing rabbit cornea","volume":"3","author":"Cintron","year":"1984","journal-title":"Curr. Eye Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1007\/BF00941763","article-title":"The expression of tenascin and fibronectin in keratoconus, scarred and normal human cornea","volume":"235","author":"Tuori","year":"1997","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1083\/jcb.96.2.474","article-title":"Fibronectin expression during myogenesis","volume":"96","author":"Gardner","year":"1983","journal-title":"J. Cell Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1083\/jcb.98.6.1926","article-title":"Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis","volume":"98","author":"Chiquet","year":"1984","journal-title":"J. Cell Biol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"23486","DOI":"10.1074\/jbc.M312694200","article-title":"Extracellular matrices of the avian ovarian follicle. Molecular characterization of chicken perlecan","volume":"279","author":"Hummel","year":"2004","journal-title":"J. Biol. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/4\/3555\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:29:52Z","timestamp":1760120992000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/4\/3555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,10]]},"references-count":69,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ijms24043555"],"URL":"https:\/\/doi.org\/10.3390\/ijms24043555","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,10]]}}}