{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T21:16:59Z","timestamp":1762809419863,"version":"build-2065373602"},"reference-count":76,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"institutional fundings of Stazione Zoologica of Napoli","award":["SZNTerzibasi","SNS_19_B_Cellerino"],"award-info":[{"award-number":["SZNTerzibasi","SNS_19_B_Cellerino"]}]},{"name":"Scuola Normale Superiore of Pisa","award":["SZNTerzibasi","SNS_19_B_Cellerino"],"award-info":[{"award-number":["SZNTerzibasi","SNS_19_B_Cellerino"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Adult neurogenesis is defined as the ability of specialized cells in the postnatal brain to produce new functional neurons and to integrate them into the already-established neuronal network. This phenomenon is common in all vertebrates and has been found to be extremely relevant for numerous processes, such as long-term memory, learning, and anxiety responses, and it has been also found to be involved in neurodegenerative and psychiatric disorders. Adult neurogenesis has been studied extensively in many vertebrate models, from fish to human, and observed also in the more basal cartilaginous fish, such as the lesser-spotted dogfish, Scyliorhinus canicula, but a detailed description of neurogenic niches in this animal is, to date, limited to the telencephalic areas. With this article, we aim to extend the characterization of the neurogenic niches of S. canicula in other main areas of the brain: we analyzed via double immunofluorescence sections of telencephalon, optic tectum, and cerebellum with markers of proliferation (PCNA) and mitosis (pH3) in conjunction with glial cell (S100\u03b2) and stem cell (Msi1) markers, to identify the actively proliferating cells inside the neurogenic niches. We also labeled adult postmitotic neurons (NeuN) to exclude double labeling with actively proliferating cells (PCNA). Lastly, we observed the presence of the autofluorescent aging marker, lipofuscin, contained inside lysosomes in neurogenic areas.<\/jats:p>","DOI":"10.3390\/ijms24043650","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T03:28:46Z","timestamp":1676258926000},"page":"3650","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Localization and Characterization of Major Neurogenic Niches in the Brain of the Lesser-Spotted Dogfish Scyliorhinus canicula"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9491-5804","authenticated-orcid":false,"given":"Sara","family":"Bagnoli","sequence":"first","affiliation":[{"name":"Biology Laboratory (BIO@SNS), Scuola Normale Superiore, 56126 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Chiavacci","sequence":"additional","affiliation":[{"name":"Biology Laboratory (BIO@SNS), Scuola Normale Superiore, 56126 Pisa, Italy"},{"name":"Biology and Evolution of Marine Organisms Department (BEOM), Stazione Zoologica Anton Dohrn, 80121 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Cellerino","sequence":"additional","affiliation":[{"name":"Biology Laboratory (BIO@SNS), Scuola Normale Superiore, 56126 Pisa, Italy"},{"name":"Fritz Lipmann Institute for Age Research, Leibniz Institute, 07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3317-1538","authenticated-orcid":false,"given":"Eva","family":"Terzibasi Tozzini","sequence":"additional","affiliation":[{"name":"Biology and Evolution of Marine Organisms Department (BEOM), Stazione Zoologica Anton Dohrn, 80121 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.stem.2015.09.003","article-title":"Adult Mammalian Neural Stem Cells and Neurogenesis: Five Decades Later","volume":"17","author":"Bond","year":"2015","journal-title":"Cell Stem Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"a018812","DOI":"10.1101\/cshperspect.a018812","article-title":"Neurogenesis in the Adult Hippocampus","volume":"7","author":"Kempermann","year":"2015","journal-title":"Cold Spring Harb. 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