{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T20:20:11Z","timestamp":1768767611301,"version":"3.49.0"},"reference-count":42,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T00:00:00Z","timestamp":1676246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"National Key R&amp;D Program of China","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"National Key R&amp;D Program of China","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"National Key R&amp;D Program of China","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"National Key R&amp;D Program of China","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"National Key R&amp;D Program of China","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"National Key R&amp;D Program of China","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"National Key R&amp;D Program of China","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"National Key R&amp;D Program of China","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"National Nature Science Foundation of China","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"National Nature Science Foundation of China","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"National Nature Science Foundation of China","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"National Nature Science Foundation of China","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"National Nature Science Foundation of China","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"National Nature Science Foundation of China","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"National Nature Science Foundation of China","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"National Nature Science Foundation of China","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"Taishan Scholars Program","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"Taishan Scholars Program","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"Taishan Scholars Program","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"Taishan Scholars Program","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"Taishan Scholars Program","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"Taishan Scholars Program","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"Taishan Scholars Program","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"Taishan Scholars Program","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"C.S, the National Ten-Thousands Talents Special Support Program to C.S., the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"the China Agriculture Research System","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"the China Agriculture Research System","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"the China Agriculture Research System","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"the China Agriculture Research System","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"the China Agriculture Research System","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"the China Agriculture Research System","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"the China Agriculture Research System","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"the China Agriculture Research System","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"National Marine Genetic Resource Center","award":["20603022021018"],"award-info":[{"award-number":["20603022021018"]}]},{"name":"National Marine Genetic Resource Center","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"National Marine Genetic Resource Center","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"National Marine Genetic Resource Center","award":["31472269"],"award-info":[{"award-number":["31472269"]}]},{"name":"National Marine Genetic Resource Center","award":["2017ASTCP-ES06"],"award-info":[{"award-number":["2017ASTCP-ES06"]}]},{"name":"National Marine Genetic Resource Center","award":["tstp20221149"],"award-info":[{"award-number":["tstp20221149"]}]},{"name":"National Marine Genetic Resource Center","award":["2020TD19"],"award-info":[{"award-number":["2020TD19"]}]},{"name":"National Marine Genetic Resource Center","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fish sex determination can be affected by environmental temperature. This process relies on temperature-sensitive proteins such as heat shock proteins (HSPs). Our previous work found that heat shock cognate proteins (HSCs) may participate in high-temperature associated sex reversal of Chinese tongue sole (Cynoglossus semilaevis). However, the role of hsc genes in responding to high temperature and affecting sex determination\/differentiation remains unclear. Here, by using C. semilaevis as model, we identified hsc70 and hsc70-like. hsc70 was abundant in the gonads with a testicular-higher expression at all gonadal development stages except for 6 months post fertilization (mpf). Intriguingly, hsc70-like showed higher expression in testes from 6 mpf on. Both long-term heat treatment during the temperature-sensitive sex-determining period and short-term heat stress at the end of this period caused different expression of hsc70\/hsc70-like between sexes. The dual-luciferase assay results also suggested that these genes can respond to high temperature rapidly in vitro. Heat treatment of C. semilaevis testis cells overexpressed with hsc70\/hsc70-like could affect the expression of sex-related genes sox9a and cyp19a1a. Our results indicated that hsc70 and hsc70-like were key regulators linking external high-temperature signals with sex differentiation in vivo and provide a new idea for understanding the mechanism by which high temperature affects sex determination\/differentiation in teleosts.<\/jats:p>","DOI":"10.3390\/ijms24043761","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T02:16:01Z","timestamp":1676340961000},"page":"3761","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Role of the Heat Shock Cognate Protein 70 Genes in Sex Determination and Differentiation of Chinese Tongue Sole (Cynoglossus semilaevis)"],"prefix":"10.3390","volume":"24","author":[{"given":"Qian","family":"Liu","sequence":"first","affiliation":[{"name":"College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leilei","family":"Tan","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Jiangsu Key Laboratory of Marine Biological Resources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxiu","family":"Ma","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaona","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6953-2203","authenticated-orcid":false,"given":"Changwei","family":"Shao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0326-4410","authenticated-orcid":false,"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1530\/rep.0.1240447","article-title":"Vertebrate sex determination: Many means to an end","volume":"124","author":"Morrish","year":"2002","journal-title":"Reproduction"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Doris, B., Judith, E.M., Catherine, L.P., Mark, K., Sarah, P.O., Tia-Lynn, A., Matthew, W.H., Jun, K., Itay, M., and Ray, M. (2014). Sex determination: Why so many ways of doing it?. PLoS Biol., 12.","DOI":"10.1371\/journal.pbio.1001899"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/hdy.1981.74","article-title":"Evolution of environmental sex determination from genotypic sex determination","volume":"47","author":"Bull","year":"1981","journal-title":"Heredity"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1146\/annurev.genet.42.110807.091714","article-title":"Weird animal genomes and the evolution of vertebrate sex and sex chromosomes","volume":"42","year":"2008","journal-title":"Annu. Rev. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0044-8486(97)00242-1","article-title":"Effect of ambient water temperature on sex determinism in the blue tilapia Oreochromis aureus","volume":"162","author":"Desprez","year":"1998","journal-title":"Aquaculture"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/(SICI)1097-010X(19961015)276:3<209::AID-JEZ5>3.0.CO;2-R","article-title":"Sex differentiation of channel catfish gonads: Normal development and effects of temperature","volume":"276","author":"Davis","year":"1996","journal-title":"J. Exp. Zool."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1677\/jme.0.0230167","article-title":"Suppression of P450 aromatase gene expression in sex-reversed males produced by rearing genetically female larvae at a high water temperature during a period of sex differentiation in the Japanese flounder (Paralichthys olivaceus)","volume":"23","author":"Kitano","year":"1999","journal-title":"J. Mol. Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1159\/000330120","article-title":"Effect of rearing temperatures during embryonic development on the phenotypic sex in zebrafish (Danio rerio)","volume":"5","author":"Abozaid","year":"2011","journal-title":"Sex Dev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1242\/jeb.205.6.711","article-title":"Oocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafish","volume":"205","author":"Daisuke","year":"2002","journal-title":"J. Exp. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S1095-6433(03)00178-8","article-title":"An aromatase inhibitor or high water temperature induce oocyte apoptosis and depletion of P450 aromatase activity in the gonads of genetic female zebrafish during sex-reversal","volume":"137","author":"Uchida","year":"2004","journal-title":"Comp. Biochem. Physiol. Part A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1002\/jez.1402730306","article-title":"Temperature and sex chromosomes govern sex ratios of the mouthbrooding Cichlid fish Oreochromis niloticus","volume":"273","author":"Baroiller","year":"1995","journal-title":"J. Exp. Zool."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/ng.2890","article-title":"Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle","volume":"46","author":"Chen","year":"2014","journal-title":"Nat. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.1016\/j.aquaculture.2013.09.010","article-title":"Effects of temperature and genotype on sex determination and sexual size dimorphism of bluegill sunfish Lepomis macrochirus","volume":"420\u2013421","author":"Wang","year":"2014","journal-title":"Aquaculture"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1093\/jnci\/92.19.1564","article-title":"Role of the heat shock response and molecular chaperones in oncogenesis and cell death","volume":"92","author":"Caroline","year":"2000","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.physiol.61.1.243","article-title":"Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology","volume":"61","author":"Feder","year":"1999","journal-title":"Annu. Rev. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S2825","DOI":"10.1073\/pnas.1506692112","article-title":"Substrate-binding domain conformational dynamics mediate Hsp70 allostery","volume":"112","author":"Zhuravleva","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.cbpb.2011.02.009","article-title":"Characterization of two heat shock proteins (Hsp70\/Hsc70) from grass carp (Ctenopharyngodon idella): Evidence for their differential gene expression, protein synthesis and secretion in LPS-challenged peripheral blood lymphocytes","volume":"159","author":"Zhang","year":"2011","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s00128-009-9908-0","article-title":"Seasonal. Effect on heat shock proteins in fish from kuwait bay","volume":"84","author":"Beg","year":"2010","journal-title":"Bull Environ. Contam. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1379\/CSC-271.1","article-title":"Expression of heat shock protein 70 in the thermally stressed antarctic clam Laternula elliptica","volume":"12","author":"Park","year":"2007","journal-title":"Cell Stress Chaperones"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5817","DOI":"10.1073\/pnas.0507903103","article-title":"Differential effects of Hsc70 and Hsp70 on the intracellular trafficking and functional expression of epithelial sodium channels","volume":"103","author":"Goldfarb","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"529","DOI":"10.3389\/fendo.2019.00529","article-title":"Involvement of heat shock proteins on the transcriptional regulation of corticotropin-releasing hormone in medaka","volume":"10","author":"Uchimura","year":"2019","journal-title":"Front Endocrinol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wang, C., Chen, X., Dai, Y., Zhang, Y., Sun, Y., and Cui, X. (2022). Comparative transcriptome analysis of heat-induced domesticated zebrafish during gonadal differentiation. BMC Genom., 23.","DOI":"10.1186\/s12863-022-01058-6"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-020-07157-x","article-title":"Early response to heat stress in Chinese tongue sole (Cynoglossus semilaevis): Performance of different sexes, candidate genes and networks","volume":"21","author":"Wang","year":"2020","journal-title":"BMC Genom."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.3389\/fgene.2019.01128","article-title":"Transcriptome of gonads from high temperature induced sex reversal during sex determination and differentiation in Chinese tongue sole, Cynoglossus semilaevis","volume":"10","author":"Wang","year":"2019","journal-title":"Front Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s10126-008-9139-0","article-title":"Artificial gynogenesis and sex determination in half-smooth tongue sole (Cynoglossus semilaevis)","volume":"11","author":"Chen","year":"2009","journal-title":"Mar. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s10126-009-9184-3","article-title":"Construction of a genetic linkage map and mapping of a female-specific DNA marker in half-smooth tongue sole (Cynoglossus semilaevis)","volume":"11","author":"Liao","year":"2009","journal-title":"Mar. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1101\/gr.162172.113","article-title":"Epigenetic modification and inheritance in sexual reversal of fish","volume":"24","author":"Shao","year":"2014","journal-title":"Genome Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1002\/j.1460-2075.1995.tb07222.x","article-title":"Identification of a regulatory motif in Hsp70 that affects ATPase activity, substrate binding and interaction with HDJ-1","volume":"14","author":"Freeman","year":"1995","journal-title":"EMBO J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.pharmthera.2012.08.014","article-title":"Comprehensive review on the HSC70 functions, interactions with related molecules and involvement in clinical diseases and therapeutic potential","volume":"136","author":"Liu","year":"2012","journal-title":"Pharmacol. Therapeut."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/j.1432-1033.1992.tb16709.x","article-title":"Molecular cloning and characterization of a constitutively expressed heat-shock-cognate hsc71 gene from rainbow trout","volume":"204","author":"Zafarullah","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0006-291X(03)01206-3","article-title":"Tissue- and stressor-specific differential expression of two hsc70 genes in carp","volume":"307","author":"Ali","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.gene.2015.02.007","article-title":"Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi","volume":"560","author":"Wang","year":"2015","journal-title":"Gene"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1111\/are.12076","article-title":"Cloning and expression of two hsc70 genes in walking catfish Clarias macrocephalus (G\u00fcnther, 1864) challenged with Aeromonas hydrophila","volume":"45","author":"Poompoung","year":"2014","journal-title":"Aquac. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"319","DOI":"10.24272\/j.issn.2095-8137.2021.406","article-title":"Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole (Cynoglossus semilaevis)","volume":"43","author":"Lu","year":"2022","journal-title":"Zool. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zhao, X., Feng, B., Wang, Q., Tang, L., Liu, Q., Ma, W., Li, C., and Shao, C. (2022). Cloning of the maternal effector gene org and its regulation by lncRNA ORG-AS in Chinese tongue sole (Cynoglossus semilaevis). Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23158605"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1042\/BJ20050412","article-title":"Heat-shock cognate 70 is required for the activation of heat-shock factor 1 in mammalian cells","volume":"392","author":"Ahn","year":"2005","journal-title":"Biochem. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.gene.2007.08.016","article-title":"Cloning and expression analysis of heat shock cognate 70 gene promoter in tiger shrimp (Penaeus monodon)","volume":"405","author":"Chuang","year":"2007","journal-title":"Gene"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s13131-011-0092-9","article-title":"Molecular cloning, characterization and expression analysis of sox9a and foxl2 genes in half-smooth tongue sole (Cynoglossus semilaevis)","volume":"30","author":"Dong","year":"2011","journal-title":"ACTA Oceanol. Sin."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s13131-013-0339-8","article-title":"Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts","volume":"32","author":"Shao","year":"2013","journal-title":"ACTA Oceanol. Sin."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.aquaculture.2008.10.034","article-title":"Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis","volume":"287","author":"Deng","year":"2009","journal-title":"Aquaculture"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jtherbio.2017.06.006","article-title":"Epigenetic control of cyp19a1a expression is critical for high temperature induced Nile tilapia masculinization","volume":"69","author":"Wang","year":"2017","journal-title":"J. Therm. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"522","DOI":"10.3389\/fgene.2019.00522","article-title":"Transcriptome profiling insights the feature of sex reversal induced by high temperature in tongue sole Cynoglossus semilaevis","volume":"10","author":"Liu","year":"2019","journal-title":"Front Genet."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/4\/3761\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:33:53Z","timestamp":1760121233000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/4\/3761"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,13]]},"references-count":42,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ijms24043761"],"URL":"https:\/\/doi.org\/10.3390\/ijms24043761","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,13]]}}}