{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:43:46Z","timestamp":1791222226086,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T00:00:00Z","timestamp":1676764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>An aromatic substrate for hydroxylation by hydroxyl radicals (\u2022OH) was investigated. The probe, N,N\u2019-(5-nitro-1,3-phenylene)-bis-glutaramide, and its hydroxylated product do not bind either iron(III) or iron(II), and so they do not interfere with the Fenton reaction. A spectrophotometric assay based on the hydroxylation of the substrate was developed. The synthesis and purification methods of this probe from previously published methodologies were improved upon, as well as the analytical procedure for monitoring the Fenton reaction through its use, enabling univocal and sensitive \u2022OH detection. The assay was utilised to demonstrate that the iron(III) complexes of long-chain fatty acids lack Fenton activity under biological conditions.<\/jats:p>","DOI":"10.3390\/ijms24044162","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T04:28:57Z","timestamp":1676867337000},"page":"4162","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Colorimetric Detection of the Hydroxyl Radical"],"prefix":"10.3390","volume":"24","author":[{"given":"Yandong","family":"Ran","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Science, King\u2019s College London, London SE1 9NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Moursy","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Science, King\u2019s College London, London SE1 9NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Hider","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Science, King\u2019s College London, London SE1 9NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9711-5183","authenticated-orcid":false,"given":"Agostino","family":"Cilibrizzi","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Science, King\u2019s College London, London SE1 9NH, UK"},{"name":"Centre for Therapeutic Innovation, University of Bath, Bath BA2 7AY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3109\/10408368109105866","article-title":"Caeruloplasmin: Physiological and pathological perspectives","volume":"14","author":"Gutteridge","year":"1981","journal-title":"Crit. 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