{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T21:35:44Z","timestamp":1772228144458,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Econom\u00eda y Competitividad (MINECO)","award":["BFU2012-31719"],"award-info":[{"award-number":["BFU2012-31719"]}]},{"name":"Ministerio de Econom\u00eda y Competitividad (MINECO)","award":["ACOMP\/2015\/042"],"award-info":[{"award-number":["ACOMP\/2015\/042"]}]},{"name":"Ministerio de Econom\u00eda y Competitividad (MINECO)","award":["PEJ-2014-P-00516"],"award-info":[{"award-number":["PEJ-2014-P-00516"]}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["BFU2012-31719"],"award-info":[{"award-number":["BFU2012-31719"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["ACOMP\/2015\/042"],"award-info":[{"award-number":["ACOMP\/2015\/042"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["PEJ-2014-P-00516"],"award-info":[{"award-number":["PEJ-2014-P-00516"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministerio de Econom\u00eda y Competitividad (MINECO)","award":["BFU2012-31719"],"award-info":[{"award-number":["BFU2012-31719"]}]},{"name":"Ministerio de Econom\u00eda y Competitividad (MINECO)","award":["ACOMP\/2015\/042"],"award-info":[{"award-number":["ACOMP\/2015\/042"]}]},{"name":"Ministerio de Econom\u00eda y Competitividad (MINECO)","award":["PEJ-2014-P-00516"],"award-info":[{"award-number":["PEJ-2014-P-00516"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We report the molecular characterization of an ethyl methanesulfonate (EMS)-induced mutation that causes albinism and lethality at the seedling stage in Arabidopsis thaliana. We identified the mutation using a mapping-by-sequencing approach that uses Fisher\u2019s exact tests to detect changes in allele frequencies among the seedlings of an F2 mapping population, which had been pooled according to their phenotypes (wild-type or mutant). After purifying genomic DNA from the plants of both pools, the two samples were sequenced using the Illumina HiSeq 2500 next-generation sequencing platform. The bioinformatic analysis allowed us to identify a point mutation that damages a conserved residue at the acceptor site of an intron of the At2g04030 gene, which encodes the chloroplast-localized AtHsp90.5 protein, a member of the HSP90 family of heat shock proteins. Our RNA-seq analysis demonstrates that the new allele alters the splicing of At2g04030 transcripts in multiple ways, leading to massive deregulation of genes encoding plastid-localized proteins. A search for protein\u2013protein interactions using the yeast two-hybrid method allowed us to identify two members of the GrpE superfamily as potential interactors of AtHsp90.5, as has previously been reported for green algae.<\/jats:p>","DOI":"10.3390\/ijms24044196","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T04:28:57Z","timestamp":1676867337000},"page":"4196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cloning of an Albino Mutation of Arabidopsis thaliana Using Mapping-by-Sequencing"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8050-7343","authenticated-orcid":false,"given":"Eva","family":"Rodr\u00edguez-Alcocer","sequence":"first","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5592-4815","authenticated-orcid":false,"given":"Erundina","family":"Ruiz-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3981-7638","authenticated-orcid":false,"given":"Ricardo","family":"Parre\u00f1o","sequence":"additional","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7480-1009","authenticated-orcid":false,"given":"C\u00e9sar","family":"Mart\u00ednez-Guardiola","sequence":"additional","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-9416","authenticated-orcid":false,"given":"Jos\u00e9 Marcos","family":"Berna","sequence":"additional","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9884-6538","authenticated-orcid":false,"given":"Ay\u00e7a","family":"\u00c7akmak Pehlivanl\u0131","sequence":"additional","affiliation":[{"name":"Department of Statistics, Mimar Sinan Fine Arts University, 34380 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0055-8843","authenticated-orcid":false,"given":"Sara","family":"Jover-Gil","sequence":"additional","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3050-4408","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Candela","sequence":"additional","affiliation":[{"name":"Instituto de Bioingenier\u00eda, Universidad Miguel Hern\u00e1ndez, Campus de Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/nmeth0809-550","article-title":"SHOREmap: Simultaneous Mapping and Mutation Identification by Deep Sequencing","volume":"6","author":"Schneeberger","year":"2009","journal-title":"Nat. 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