{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:23:16Z","timestamp":1783455796741,"version":"3.55.0"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK118611"],"award-info":[{"award-number":["DK118611"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AG078768"],"award-info":[{"award-number":["AG078768"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ohio University Institutional Fund","award":["DK118611"],"award-info":[{"award-number":["DK118611"]}]},{"name":"Ohio University Institutional Fund","award":["AG078768"],"award-info":[{"award-number":["AG078768"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Dietary lipids induce apolipoprotein A4 (APOA4) production and brown adipose tissue (BAT) thermogenesis. Administration of exogenous APOA4 elevates BAT thermogenesis in chow-fed mice, but not high-fat diet (HFD)-fed mice. Chronic feeding of HFD attenuates plasma APOA4 production and BAT thermogenesis in wildtype (WT) mice. In light of these observations, we sought to determine whether steady production of APOA4 could keep BAT thermogenesis elevated, even in the presence of HFD consumption, with an aim toward eventual reduction of body weight, fat mass and plasma lipid levels. Transgenic mice with overexpression of mouse APOA4 in the small intestine (APOA4-Tg mice) produce greater plasma APOA4 than their WT controls, even when fed an atherogenic diet. Thus, we used these mice to investigate the correlation of levels of APOA4 and BAT thermogenesis during HFD consumption. The hypothesis of this study was that overexpression of mouse APOA4 in the small intestine and increased plasma APOA4 production would increase BAT thermogenesis and consequently reduce fat mass and plasma lipids of HFD-fed obese mice. To test this hypothesis, BAT thermogenic proteins, body weight, fat mass, caloric intake, and plasma lipids in male APOA4-Tg mice and WT mice fed either a chow diet or a HFD were measured. When fed a chow diet, APOA4 levels were elevated, plasma triglyceride (TG) levels were reduced, and BAT levels of UCP1 trended upward, while body weight, fat mass, caloric intake, and plasma lipids were comparable between APOA4-Tg and WT mice. After a four-week feeding of HFD, APOA4-Tg mice maintained elevated plasma APOA4 and reduced plasma TG, but UCP1 levels in BAT were significantly elevated in comparison to WT controls; body weight, fat mass and caloric intake were still comparable. After 10-week consumption of HFD, however, while APOA4-Tg mice still exhibited increased plasma APOA4, UCP1 levels and reduced TG levels, a reduction in body weight, fat mass and levels of plasma lipids and leptin were finally observed in comparison to their WT controls and independent of caloric intake. Additionally, APOA4-Tg mice exhibited increased energy expenditure at several time points when measured during the 10-week HFD feeding. Thus, overexpression of APOA4 in the small intestine and maintenance of elevated levels of plasma APOA4 appear to correlate with elevation of UCP1-dependent BAT thermogenesis and subsequent protection against HFD-induced obesity in mice.<\/jats:p>","DOI":"10.3390\/ijms24044231","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T02:07:44Z","timestamp":1676945264000},"page":"4231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increased BAT Thermogenesis in Male Mouse Apolipoprotein A4 Transgenic Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2085-3591","authenticated-orcid":false,"given":"Zachary","family":"LaRussa","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsuan-Chih N.","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"West","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4247-9307","authenticated-orcid":false,"given":"Kevin","family":"Wisniewski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Tso","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen T.","family":"Coschigano","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0252-0267","authenticated-orcid":false,"given":"Chunmin C.","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, and Diabetes Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Prevalence of Obesity and Severe Obesity Among Adults: United States, 2017\u20132018","volume":"360","author":"Hales","year":"2020","journal-title":"NCHS Data Brief"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.vph.2006.08.010","article-title":"Cardiovascular Disease 2005\u2014The Global Picture","volume":"45","author":"Callow","year":"2006","journal-title":"Vascul. Pharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/S0140-6736(21)01919-X","article-title":"Obesity Management as a Primary Treatment Goal for Type 2 Diabetes: Time to Reframe the Conversation","volume":"399","author":"Lingvay","year":"2022","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/0026-0495(85)90214-8","article-title":"Energy Balance and Brown Fat Activity in Rats Fed Cafeteria Diets or High-Fat, Semisynthetic Diets at Several Levels of Intake","volume":"34","author":"Rothwell","year":"1985","journal-title":"Metabolism"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"R347","DOI":"10.1152\/ajpregu.1983.244.3.R347","article-title":"Altered Sympathetic Activity during Development of Diet-Induced Obesity in Rat","volume":"244","author":"Levin","year":"1983","journal-title":"Am. J. Physiol.\u2014Regul. Integr. Comp. Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Vijgen, G.H.E.J., Bouvy, N.D., Teule, G.J.J., Brans, B., Schrauwen, P., and van Marken Lichtenbelt, W.D. (2011). Brown Adipose Tissue in Morbidly Obese Subjects. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017247"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1056\/NEJMoa0808949","article-title":"Functional Brown Adipose Tissue in Healthy Adults","volume":"360","author":"Virtanen","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.cmet.2014.12.009","article-title":"Activation of Human Brown Adipose Tissue by a \u03923-Adrenergic Receptor Agonist","volume":"21","author":"Cypess","year":"2015","journal-title":"Cell Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/nm.2297","article-title":"Brown Adipose Tissue Activity Controls Triglyceride Clearance","volume":"17","author":"Bartelt","year":"2011","journal-title":"Nat. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1152\/ajpendo.00691.2006","article-title":"Unexpected Evidence for Active Brown Adipose Tissue in Adult Humans","volume":"293","author":"Nedergaard","year":"2007","journal-title":"Am. J. Physiol.\u2014Endocrinol. Metab."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1042\/bj2770119","article-title":"Noradrenaline Stimulates Glucose Transport in Rat Brown Adipocytes by Activating Thermogenesis: Evidence That Fatty Acid Activation of Mitochondrial Respiration Enhances Glucose Transport","volume":"277","author":"Marette","year":"1991","journal-title":"Biochem. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.2337\/db15-0146","article-title":"Human Brown Adipose Tissue: What We Have Learned so Far","volume":"64","author":"Betz","year":"2015","journal-title":"Diabetes"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Blouet, C., and Schwartz, G.J. (2012). Duodenal Lipid Sensing Activates Vagal Afferents to Regulate Non-Shivering Brown Fat Thermogenesis in Rats. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0051898"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Vijgen, G.H.E.J., Bouvy, N.D., Leenen, L., Rijkers, K., Cornips, E., Majoie, M., Brans, B., and van Marken Lichtenbelt, W.D. (2013). Vagus Nerve Stimulation Increases Energy Expenditure: Relation to Brown Adipose Tissue Activity. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0077221"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1152\/physrev.00015.2003","article-title":"Brown Adipose Tissue: Function and Physiological Significance","volume":"84","author":"Cannon","year":"2004","journal-title":"Physiol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S36","DOI":"10.1038\/ijo.2010.182","article-title":"Sympathetic and Sensory Innervation of Brown Adipose Tissue","volume":"34","author":"Bartness","year":"2010","journal-title":"Int. J. Obes."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1152\/ajpregu.00648.2004","article-title":"Sensory or Sympathetic White Adipose Tissue Denervation Differentially Affects Depot Growth and Cellularity","volume":"288","author":"Shi","year":"2005","journal-title":"Am. J. Physiol.\u2014Regul. Integr. Comp. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1146\/annurev.bi.40.070171.002341","article-title":"biochemistry of Catecholamines","volume":"40","author":"Molinoff","year":"1971","journal-title":"Annu. Rev. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.cmet.2009.03.012","article-title":"AMPK: An Emerging Drug Target for Diabetes and the Metabolic Syndrome","volume":"9","author":"Zhang","year":"2009","journal-title":"Cell Metab."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1007\/s00018-010-0454-z","article-title":"AMP-Activated Protein Kinase and Its Downstream Transcriptional Pathways","volume":"67","author":"Auwerx","year":"2010","journal-title":"Cell Mol. Life Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1126\/science.1100747","article-title":"Fat Mobilization in Adipose Tissue Is Promoted by Adipose Triglyceride Lipase","volume":"306","author":"Zimmermann","year":"2004","journal-title":"Science"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1194\/jlr.R200009-JLR200","article-title":"Hormone-Sensitive Lipase: Control of Intracellular Tri-(Di-)Acylglycerol and Cholesteryl Ester Hydrolysis","volume":"43","author":"Kraemer","year":"2002","journal-title":"J. Lipid Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.molmed.2015.07.003","article-title":"Regulation of Brown Fat by AMP-Activated Protein Kinase","volume":"21","author":"Kooijman","year":"2015","journal-title":"Trends Mol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1016\/j.bbamcr.2016.04.013","article-title":"UCPs, at the Interface between Bioenergetics and Metabolism","volume":"1863","author":"Bouillaud","year":"2016","journal-title":"Biochim. Biophys. Acta\u2014Mol. Cell Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"G472","DOI":"10.1152\/ajpgi.00098.2014","article-title":"ApoA-IV: Current and Emerging Roles in Intestinal Lipid Metabolism, Glucose Homeostasis, and Satiety","volume":"308","author":"Kohan","year":"2015","journal-title":"Am. J. Physiol.\u2014Gastrointest. Liver Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1016\/S0022-2275(20)42345-4","article-title":"Transport of Lipid and Apolipoproteins A-I and A-IV in Intestinal Lymph of the Rat","volume":"31","author":"Hayashi","year":"1990","journal-title":"J. Lipid Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"R1490","DOI":"10.1152\/ajpregu.00329.2007","article-title":"Interaction of Apolipoprotein AIV with Cholecystokinin on the Control of Food Intake","volume":"293","author":"Lo","year":"2007","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1194\/jlr.R052753","article-title":"Apolipoprotein A-IV: A Protein Intimately Involved in Metabolism","volume":"56","author":"Wang","year":"2015","journal-title":"J. Lipid Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1906","DOI":"10.1172\/JCI119358","article-title":"Reduced Aortic Lesions and Elevated High Density Lipoprotein Levels in Transgenic Mice Overexpressing Mouse Apolipoprotein A-IV","volume":"99","author":"Cohen","year":"1997","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4266","DOI":"10.1016\/S0021-9258(19)39557-2","article-title":"Lipoprotein ApoC-II Activation of Lipoprotein Lipase. Modulation by Apolipoprotein A-IV","volume":"265","author":"Goldberg","year":"1990","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"bqaa042","DOI":"10.1210\/endocr\/bqaa042","article-title":"Apolipoprotein A-IV Enhances Fatty Acid Uptake by Adipose Tissues of Male Mice via Sympathetic Activation","volume":"161","author":"Zhu","year":"2020","journal-title":"Endocrinology"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Pence, S., Zhu, Q., Binne, E., Liu, M., Shi, H., and Lo, C.C. (2019). Reduced Diet-Induced Thermogenesis in Apolipoprotein a-IV Deficient Mice. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20133176"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1093\/jn\/133.4.1081","article-title":"A Controlled High-Fat Diet Induces an Obese Syndrome in Rats","volume":"133","author":"Woods","year":"2003","journal-title":"J. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"R1155","DOI":"10.1152\/ajpregu.2001.280.4.R1155","article-title":"Adaptation of Intestinal Production of Apolipoprotein A-IV during Chronic Feeding of Lipid","volume":"280","author":"Kalogeris","year":"2001","journal-title":"Am. J. Physiol.\u2014Regul. Integr. Comp. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1152\/ajpendo.00448.2003","article-title":"Obesity Induced by a High-Fat Diet Downregulates Apolipoprotein A-IV Gene Expression in Rat Hypothalamus","volume":"287","author":"Liu","year":"2004","journal-title":"Am. J. Physiol.\u2014Endocrinol. Metab."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0016-5085(90)91285-E","article-title":"Sensitivity of Serum Apolipoprotein A-IV Levels to Changes in Dietary Fat Content","volume":"98","author":"Weinberg","year":"1990","journal-title":"Gastroenterology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1172\/JCI117163","article-title":"Intestinal Expression of Human Apolipoprotein A-IV in Transgenic Mice Fails to Influence Dietary Lipid Absorption or Feeding Behavior","volume":"93","author":"Bisgaier","year":"1994","journal-title":"J. Clin. Investig."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5857","DOI":"10.1210\/en.2012-1427","article-title":"Apolipoprotein AIV Requires Cholecystokinin and Vagal Nerves to Suppress Food Intake","volume":"153","author":"Lo","year":"2012","journal-title":"Endocrinology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"652444","DOI":"10.3389\/fendo.2021.652444","article-title":"Sex Differences in Brown Adipose Tissue Function: Sex Hormones, Glucocorticoids, and Their Crosstalk","volume":"12","author":"Kaikaew","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4061","DOI":"10.1073\/pnas.95.7.4061","article-title":"Diet-Induced Changes in Uncoupling Proteins in Obesity-Prone and Obesity-Resistant Strains of Mice","volume":"95","author":"Surwit","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1152\/ajpregu.00034.2017","article-title":"Energy Homeostasis in Apolipoprotein AIV and Cholecystokinin-Deficient Mice","volume":"313","author":"Weng","year":"2017","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Pence, S., Larussa, Z., Shen, Z., Liu, M., Coschigano, K.T., Shi, H., and Lo, C.C. (2021). Central Apolipoprotein A-Iv Stimulates Thermogenesis in Brown Adipose Tissue. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031221"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.physbeh.2008.05.022","article-title":"Characterization of Apolipoprotein A-IV in Brain Areas Involved in Energy Homeostasis","volume":"95","author":"Shen","year":"2008","journal-title":"Physiol. Behav."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1161\/CIRCRESAHA.115.306647","article-title":"Role of Brown Fat in Lipoprotein Metabolism and Atherosclerosis","volume":"118","author":"Hoeke","year":"2016","journal-title":"Circ. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6356","DOI":"10.1038\/ncomms7356","article-title":"Brown Fat Activation Reduces Hypercholesterolaemia and Protects from Atherosclerosis Development","volume":"6","author":"Boon","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1038\/ijo.2010.177","article-title":"Metabolic Consequences of the Presence or Absence of the Thermogenic Capacity of Brown Adipose Tissue in Mice (and Probably in Humans)","volume":"34","author":"Cannon","year":"2010","journal-title":"Int. J. Obes."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/j.cmet.2016.04.029","article-title":"Brown Adipose Tissue Activation Is Linked to Distinct Systemic Effects on Lipid Metabolism in Humans","volume":"23","author":"Chondronikola","year":"2016","journal-title":"Cell Metab."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1152\/ajpcell.1990.258.4.C673","article-title":"Lipoprotein Lipase: Cellular Origin and Functional Distribution","volume":"258","author":"Camps","year":"1990","journal-title":"Am. J. Physiol.\u2014Cell Physiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1042\/bj3110729","article-title":"Acute Cold-Induced Suppression of Ob (Obese) Gene Expression in White Adipose Tissue of Mice: Mediation by the Sympathetic System","volume":"311","author":"Trayhurn","year":"1995","journal-title":"Biochem. J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"E155","DOI":"10.1152\/ajpendo.1988.254.2.E155","article-title":"Cold-Induced \u03b2-Adrenergic Recruitment of Lipoprotein Lipase in Brown Fat Is Due to Increased Transcription","volume":"254","author":"Carneheim","year":"1988","journal-title":"Am. J. Physiol.\u2014Endocrinol. Metab."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"773","DOI":"10.3945\/jn.109.115964","article-title":"APOA1 and APOA4 Gene Polymorphisms Influence the Effects of Dietary Fat on LDL Particle Size and Oxidation in Healthy Young Adults","volume":"140","author":"Gomez","year":"2010","journal-title":"J. Nutr."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"G628","DOI":"10.1152\/ajpgi.00225.2011","article-title":"Apolipoprotein A-IV Regulates Chylomicron Metabolism-Mechanism and Function","volume":"302","author":"Kohan","year":"2012","journal-title":"AJP Gastrointest. Liver Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1016\/S0022-2275(20)37153-4","article-title":"Decreased HDL Cholesterol Levels but Normal Lipid Absorption, Growth, and Feeding Behavior in Apolipoprotein A-IV Knockout Mice","volume":"38","author":"Weinstock","year":"1997","journal-title":"J. Lipid Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1194\/jlr.M017418","article-title":"Impact of Murine Intestinal Apolipoprotein A-IV Expression on Regional Lipid Absorption, Gene Expression, and Growth","volume":"52","author":"Simon","year":"2011","journal-title":"J. Lipid Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/nm1195-1155","article-title":"Leptin Levels in Human and Rodent: Measurement of Plasma Leptin and Ob RNA in Obese and Weight-Reduced Subjects","volume":"1","author":"Maffei","year":"1995","journal-title":"Nat. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1038\/oby.2002.130","article-title":"Fat Intake Affects Adiposity, Comorbidity Factors, and Energy Metabolism of Sprague-Dawley Rats","volume":"10","author":"Ghibaudi","year":"2002","journal-title":"Obes. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1582-4934.2010.01045.x","article-title":"Apolipoprotein A-I Possesses an Anti-Obesity Effect Associated with Increase of Energy Expenditure and up-Regulation of UCP1 in Brown Fat","volume":"15","author":"Ruan","year":"2011","journal-title":"J. Cell. Mol. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.prostaglandins.2018.10.004","article-title":"ping Apolipoprotein A-IV: A Potential Therapeutic Target for Atherosclerosis","volume":"139","author":"Peng","year":"2018","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1161\/ATVBAHA.118.311978","article-title":"Reverse Cholesterol Transport and Atherosclerosis: Heterogeneous Observations by Vascular Bed and Plaque Architecture","volume":"39","author":"Rohatgi","year":"2019","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"G1336","DOI":"10.1152\/ajpgi.00325.2010","article-title":"Effect of Peripheral Administration of Cholecystokinin on Food Intake in Apolipoprotein AIV Knockout Mice","volume":"302","author":"Yoshimichi","year":"2012","journal-title":"AJP Gastrointest. Liver Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1038\/s41592-019-0513-9","article-title":"Progress and Challenges in Analyzing Rodent Energy Expenditure","volume":"16","author":"Ravussin","year":"2019","journal-title":"Nat. Methods"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nmeth.1806","article-title":"A Guide to Analysis of Mouse Energy Metabolism","volume":"9","author":"Speakman","year":"2012","journal-title":"Nat. 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