{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T07:16:59Z","timestamp":1761808619402,"version":"build-2065373602"},"reference-count":81,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Intramural Research Programme of the Josip Juraj Strossmayer University","award":["IP15-2020-MEFOS","IP6-2021-MEFOS","UIP-2019-04-3494"],"award-info":[{"award-number":["IP15-2020-MEFOS","IP6-2021-MEFOS","UIP-2019-04-3494"]}]},{"DOI":"10.13039\/501100004488","name":"Croatian Science Foundation project","doi-asserted-by":"publisher","award":["IP15-2020-MEFOS","IP6-2021-MEFOS","UIP-2019-04-3494"],"award-info":[{"award-number":["IP15-2020-MEFOS","IP6-2021-MEFOS","UIP-2019-04-3494"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Psoriasis vulgaris (PV) is an autoinflammatory dermatosis of unknown etiology. Current evidence suggests a pathogenic role of \u03b3\u03b4T cells, but the growing complexity of this population has made the offending subset difficult to pinpoint. The work on \u03b3\u03b4TCRint and \u03b3\u03b4TCRhi subsets, which express intermediate and high levels of \u03b3\u03b4TCR at their surface, respectively, is particularly scarce, leaving their inner workings in PV essentially unresolved. We have shown here that the \u03b3\u03b4TCRint\/\u03b3\u03b4TCRhi cell composition and their transcriptom are related to the differential miRNA expression by performing a targeted miRNA and mRNA quantification (RT-qPCR) in multiplexed, flow-sorted \u03b3\u03b4 blood T cells from healthy controls (n = 14) and patients with PV (n = 13). A significant loss of miR-20a in bulk \u03b3\u03b4T cells (~fourfold decrease, PV vs. controls) largely mirrored increasing V\u03b41-V\u03b42- and \u03b3\u03b4intV\u03b41-V\u03b42- cell densities in the bloodstream, culminating in a relative excess of \u03b3\u03b4intV\u03b41-V\u03b42- cells for PV. Transcripts encoding DNA-binding factors (ZBTB16), cytokine receptors (IL18R1), and cell adhesion molecules (SELPLG) were depleted in the process, closely tracking miR-20a availability in bulk \u03b3\u03b4 T-cell RNA. Compared to controls, PV was also associated with enhanced miR-92b expression (~13-fold) in bulk \u03b3\u03b4T cells that lacked association with the \u03b3\u03b4T cell composition. The miR-29a and let-7c expressions remained unaltered in case\u2013control comparisons. Overall, our data expand the current landscape of the peripheral \u03b3\u03b4T cell composition, underlining changes in its mRNA\/miRNA transcriptional circuits that may inform PV pathogenesis.<\/jats:p>","DOI":"10.3390\/ijms24054323","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T03:32:17Z","timestamp":1677036737000},"page":"4323","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The miR-20a\/miR-92b Profile Is Associated with Circulating \u03b3\u03b4 T-Cell Perturbations in Mild Psoriasis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0136-7720","authenticated-orcid":false,"given":"Stana","family":"Toki\u0107","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pharmacy, Faculty of Medicine, University of Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Jirou\u0161","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Plu\u017eari\u0107","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, University Hospital Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5734-5849","authenticated-orcid":false,"given":"Martina","family":"Mihalj","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, University Hospital Osijek, 31000 Osijek, Croatia"},{"name":"Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija","family":"\u0160ola","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, University Hospital Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Tolu\u0161i\u0107 Levak","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, University Hospital Osijek, 31000 Osijek, Croatia"},{"name":"Department of Histology and Embryology, Faculty of Medicine, University of Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Glava\u0161","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Faculty of Medicine, University of Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Balogh","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Faculty of Medicine, University of Pecs, 7622 Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"\u0160tefani\u0107","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine and Oncology, Faculty of Medicine, University of Osijek, 31000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1056\/NEJMra0804595","article-title":"Psoriasis","volume":"361","author":"Nestle","year":"2009","journal-title":"N. 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