{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:12:27Z","timestamp":1780337547130,"version":"3.54.1"},"reference-count":57,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011852","name":"Paracelsus Medical University Salzburg","doi-asserted-by":"publisher","award":["E-18\/27\/145-WAL"],"award-info":[{"award-number":["E-18\/27\/145-WAL"]}],"id":[{"id":"10.13039\/501100011852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Psoriasis is an inflammatory skin disease characterized by increased neo-vascularization, keratinocyte hyperproliferation, a pro-inflammatory cytokine milieu and immune cell infiltration. Diacerein is an anti-inflammatory drug, modulating immune cell functions, including expression and production of cytokines, in different inflammatory conditions. Therefore, we hypothesized that topical diacerein has beneficial effects on the course of psoriasis. The current study aimed to evaluate the effect of topical diacerein on imiquimod (IMQ)-induced psoriasis in C57BL\/6 mice. Topical diacerein was observed to be safe without any adverse side effects in healthy or psoriatic animals. Our results demonstrated that diacerein significantly alleviated the psoriasiform-like skin inflammation over a 7-day period. Furthermore, diacerein significantly diminished the psoriasis-associated splenomegaly, indicating a systemic effect of the drug. Remarkably, we observed significantly reduced infiltration of CD11c+ dendritic cells (DCs) into the skin and spleen of psoriatic mice with diacerein treatment. As CD11c+ DCs play a pivotal role in psoriasis pathology, we consider diacerein to be a promising novel therapeutic candidate for psoriasis.<\/jats:p>","DOI":"10.3390\/ijms24054324","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T03:32:17Z","timestamp":1677036737000},"page":"4324","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Topical Diacerein Decreases Skin and Splenic CD11c+ Dendritic Cells in Psoriasis"],"prefix":"10.3390","volume":"24","author":[{"given":"Susanne M.","family":"Brunner","sequence":"first","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor Metabolism, Department of Pediatrics, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-1640","authenticated-orcid":false,"given":"Andrea","family":"Ramspacher","sequence":"additional","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor Metabolism, Department of Pediatrics, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Rieser","sequence":"additional","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor Metabolism, Department of Pediatrics, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Leitner","sequence":"additional","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor Metabolism, Department of Pediatrics, University Hospital of the Paracelsus Medical 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Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Wimmer","sequence":"additional","affiliation":[{"name":"EB House Austria, Research Program for Molecular Therapy of Genodermatoses, Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Koller","sequence":"additional","affiliation":[{"name":"Research Program for Experimental Dermatology and Glaucoma Research, Department of Ophthalmology and Optometry, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8558-9031","authenticated-orcid":false,"given":"Josefina","family":"Pi\u00f1\u00f3n Hofbauer","sequence":"additional","affiliation":[{"name":"EB House Austria, Research Program for Molecular Therapy of Genodermatoses, Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8266-5513","authenticated-orcid":false,"given":"Thomas K.","family":"Felder","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann W.","family":"Bauer","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1198-4776","authenticated-orcid":false,"given":"Barbara","family":"Kofler","sequence":"additional","affiliation":[{"name":"Research Program for Receptor Biochemistry and Tumor 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Exp. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1038\/jid.2011.427","article-title":"Physical and mental impact of psoriasis severity as measured by the compact Short Form-12 Health Survey (SF-12) quality of life tool","volume":"132","author":"Grozdev","year":"2012","journal-title":"J. Investig. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1111\/jdv.13701","article-title":"The triad psoriasis-obesity-adipokine profile","volume":"30","author":"Coimbra","year":"2016","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s00403-006-0707-8","article-title":"The importance of disease associations and concomitant therapy for the long-term management of psoriasis patients","volume":"298","author":"Mrowietz","year":"2006","journal-title":"Arch. Dermatol. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kamata, M., and Tada, Y. (2020). Efficacy and Safety of Biologics for Psoriasis and Psoriatic Arthritis and Their Impact on Comorbidities: A Literature Review. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21051690"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-immunol-032713-120225","article-title":"Immunology of psoriasis","volume":"32","author":"Lowes","year":"2014","journal-title":"Annu. Rev. Immunol."},{"key":"ref_7","first-page":"278","article-title":"Diagnosis and management of psoriasis","volume":"63","author":"Kim","year":"2017","journal-title":"Can. Fam. Physician."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5836","DOI":"10.4049\/jimmunol.0802999","article-title":"Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23\/IL-17 axis","volume":"182","author":"Mourits","year":"2009","journal-title":"J. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Swindell, W.R., Johnston, A., Carbajal, S., Han, G., Wohn, C., Lu, J., Xing, X., Nair, R.P., Voorhees, J.J., and Elder, J.T. (2011). Genome-wide expression profiling of five mouse models identifies similarities and differences with human psoriasis. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0018266"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1177\/1759720X09359104","article-title":"Effects of diacerein at the molecular level in the osteoarthritis disease process","volume":"2","author":"Pelletier","year":"2010","journal-title":"Ther. Adv. Musculoskelet Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s40266-016-0347-4","article-title":"Diacerein: Benefits, Risks and Place in the Management of Osteoarthritis. An Opinion-Based Report from the ESCEO","volume":"33","author":"Pavelka","year":"2016","journal-title":"Drugs Aging"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1053\/joca.1998.0201","article-title":"Anti-interleukin-1 effects of diacerein and rhein in human osteoarthritic synovial tissue and cartilage cultures","volume":"7","author":"Yaron","year":"1999","journal-title":"Osteoarthr. Cartil."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.freeradbiomed.2014.04.001","article-title":"Rhein exerts pro- and anti-inflammatory actions by targeting IKKbeta inhibition in LPS-activated macrophages","volume":"72","author":"Gao","year":"2014","journal-title":"Free Radic Biol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1111\/j.2042-7158.1987.tb05131.x","article-title":"Rhein: An anthraquinone that modulates superoxide anion production from human neutrophils","volume":"39","author":"Mian","year":"1987","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mohan, G.C., Zhang, H., Bao, L., Many, B., and Chan, L.S. (2017). Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0173981"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/1750-1172-8-69","article-title":"Topical diacerein for epidermolysis bullosa: A randomized controlled pilot study","volume":"8","author":"Wally","year":"2013","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.jaad.2018.01.019","article-title":"Diacerein orphan drug development for epidermolysis bullosa simplex: A phase 2\/3 randomized, placebo-controlled, double-blind clinical trial","volume":"78","author":"Wally","year":"2018","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1186\/s13023-018-0940-1","article-title":"Basal pharmacokinetic parameters of topically applied diacerein in pediatric patients with generalized severe epidermolysis bullosa simplex","volume":"13","author":"Ablinger","year":"2018","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"a015354","DOI":"10.1101\/cshperspect.a015354","article-title":"Psoriasis","volume":"4","author":"Villanova","year":"2014","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.biopha.2017.08.053","article-title":"Diacerein inhibits Estradiol-benzoate induced cervical hyperkeratosis in female rats","volume":"95","author":"Refaie","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.2165\/00003088-199835050-00002","article-title":"Clinical pharmacokinetics of diacerein","volume":"35","author":"Nicolas","year":"1998","journal-title":"Clin. Pharmacokinet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s11926-001-0061-y","article-title":"Diacerein as a disease-modulating agent in osteoarthritis","volume":"3","author":"Falgarone","year":"2001","journal-title":"Curr Rheumatol. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s40264-015-0266-z","article-title":"Benefit-risk assessment of diacerein in the treatment of osteoarthritis","volume":"38","author":"Panova","year":"2015","journal-title":"Drug Saf."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/08982104.2018.1430831","article-title":"Investigating superiority of novel bilosomes over niosomes in the transdermal delivery of diacerein: In vitro characterization, ex vivo permeation and in vivo skin deposition study","volume":"29","author":"Aziz","year":"2019","journal-title":"J. Liposome Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1080\/10717544.2018.1451572","article-title":"Fabrication of novel elastosomes for boosting the transdermal delivery of diacerein: Statistical optimization, ex-vivo permeation, in-vivo skin deposition and pharmacokinetic assessment compared to oral formulation","volume":"25","author":"Aziz","year":"2018","journal-title":"Drug Deliv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3109\/08982104.2015.1029495","article-title":"Diacerein niosomal gel for topical delivery: Development, in vitro and in vivo assessment","volume":"26","author":"Lila","year":"2016","journal-title":"J. Liposome Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Jabeen, M., Boisgard, A.S., Danoy, A., El Kholti, N., Salvi, J.P., Boulieu, R., Fromy, B., Verrier, B., and Lamrayah, M. (2020). Advanced Characterization of Imiquimod-Induced Psoriasis-Like Mouse Model. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12090789"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5625","DOI":"10.2147\/IJN.S170832","article-title":"Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles","volume":"13","author":"Sun","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4103\/0976-0105.177703","article-title":"A simple practice guide for dose conversion between animals and human","volume":"7","author":"Nair","year":"2016","journal-title":"J. Basic Clin. Pharm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s12016-016-8535-x","article-title":"The Inflammatory Response in Psoriasis: A Comprehensive Review","volume":"50","author":"Deng","year":"2016","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1111\/exd.13746","article-title":"Development of psoriasis by continuous neutrophil infiltration into the epidermis","volume":"27","author":"Katayama","year":"2018","journal-title":"Exp. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"200","DOI":"10.3389\/fimmu.2018.00200","article-title":"IL-36gamma Is a Strong Inducer of IL-23 in Psoriatic Cells and Activates Angiogenesis","volume":"9","author":"Bridgewood","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6892","DOI":"10.3748\/wjg.v21.i22.6892","article-title":"Nonalcoholic fatty liver disease, spleen and psoriasis: New aspects of low-grade chronic inflammation","volume":"21","author":"Balato","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kaiser, H., Kvist-Hansen, A., Krakauer, M., Gortz, P.M., Henningsen, K.M.A., Wang, X., Becker, C., Zachariae, C., Skov, L., and Hansen, P.R. (2021). Association between Vascular Inflammation and Inflammation in Adipose Tissue, Spleen, and Bone Marrow in Patients with Psoriasis. Life, 11.","DOI":"10.3390\/life11040305"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"390","DOI":"10.2340\/00015555-2873","article-title":"Systemic Inflammation and Evidence of a Cardio-splenic Axis in Patients with Psoriasis","volume":"98","author":"Hjuler","year":"2018","journal-title":"Acta Derm. Venereol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.12688\/f1000research.19144.1","article-title":"Psoriatic arthritis","volume":"8","author":"Ocampo","year":"2019","journal-title":"F1000Res"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s00403-012-1272-y","article-title":"Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress","volume":"304","author":"Baek","year":"2012","journal-title":"Arch. Dermatol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13073-017-0415-3","article-title":"Imiquimod has strain-dependent effects in mice and does not uniquely model human psoriasis","volume":"9","author":"Swindell","year":"2017","journal-title":"Genome Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100115","DOI":"10.1016\/j.xpro.2020.100115","article-title":"Protocol for Flow Cytometric Detection of Immune Cell Infiltration in the Epidermis and Dermis of a Psoriasis Mouse Model","volume":"1","author":"Lou","year":"2020","journal-title":"STAR Protoc."},{"key":"ref_40","first-page":"1030","article-title":"Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model","volume":"17","author":"Surcel","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.4049\/jimmunol.173.5.3051","article-title":"Identification and characterization of pDC-like cells in normal mouse skin and melanomas treated with imiquimod","volume":"173","author":"Palamara","year":"2004","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1034\/j.1600-0625.2000.009001001.x","article-title":"Role of neutrophils in induction of acute inflammation in T-cell-mediated immune dermatosis, psoriasis: A neutrophil-associated inflammation-boosting loop","volume":"9","author":"Terui","year":"2000","journal-title":"Exp. Dermatol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e321","DOI":"10.1111\/bjd.15533","article-title":"Interleukin (IL)-17A and IL-22-producing neutrophils in psoriatic skin","volume":"177","author":"Honore","year":"2017","journal-title":"Br. J. Dermatol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s12016-018-8702-3","article-title":"The Immunologic Role of IL-17 in Psoriasis and Psoriatic Arthritis Pathogenesis","volume":"55","author":"Blauvelt","year":"2018","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1172\/JCI29441","article-title":"Misbehaving macrophages in the pathogenesis of psoriasis","volume":"116","author":"Clark","year":"2006","journal-title":"J. Clin. Investig."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/1348-0421.12228","article-title":"Functions and development of red pulp macrophages","volume":"59","author":"Kurotaki","year":"2015","journal-title":"Microbiol. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"60","DOI":"10.5483\/BMBRep.2014.47.2.014","article-title":"The pathophysiological role of dendritic cell subsets in psoriasis","volume":"47","author":"Kim","year":"2014","journal-title":"BMB Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"19057","DOI":"10.1073\/pnas.0509736102","article-title":"Increase in TNF-alpha and inducible nitric oxide synthase-expressing dendritic cells in psoriasis and reduction with efalizumab (anti-CD11a)","volume":"102","author":"Lowes","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"642","DOI":"10.3389\/fphar.2018.00642","article-title":"Molecular Mechanisms of T Cells Activation by Dendritic Cells in Autoimmune Diseases","volume":"9","author":"Tai","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/j.1600-065X.2009.00773.x","article-title":"Molecular mechanisms of T-cell receptor and costimulatory molecule ligation\/blockade in autoimmune disease therapy","volume":"229","author":"Podojil","year":"2009","journal-title":"Immunol. Rev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.4049\/jimmunol.0803721","article-title":"IL-6 signaling in psoriasis prevents immune suppression by regulatory T cells","volume":"183","author":"Goodman","year":"2009","journal-title":"J. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1084\/jem.20030451","article-title":"Increased expression of interleukin 23 p19 and p40 in lesional skin of patients with psoriasis vulgaris","volume":"199","author":"Lee","year":"2004","journal-title":"J. Exp. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1056\/NEJMoa030409","article-title":"Etanercept as monotherapy in patients with psoriasis","volume":"349","author":"Leonardi","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.jid.2017.08.015","article-title":"Lack of Galanin Receptor 3 Alleviates Psoriasis by Altering Vascularization, Immune Cell Infiltration, and Cytokine Expression","volume":"138","author":"Locker","year":"2018","journal-title":"J. Investig. Dermatol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Yu, Y.R., O'Koren, E.G., Hotten, D.F., Kan, M.J., Kopin, D., Nelson, E.R., Que, L., and Gunn, M.D. (2016). A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0150606"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/cyto.a.22012","article-title":"A novel Ly6C\/Ly6G-based strategy to analyze the mouse splenic myeloid compartment","volume":"81","author":"Rose","year":"2012","journal-title":"Cytom. A"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"100029","DOI":"10.1016\/j.xpro.2020.100029","article-title":"Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry","volume":"1","author":"Liu","year":"2020","journal-title":"STAR Protoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/5\/4324\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:38:44Z","timestamp":1760121524000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/5\/4324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,21]]},"references-count":57,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["ijms24054324"],"URL":"https:\/\/doi.org\/10.3390\/ijms24054324","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,21]]}}}