{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:16:04Z","timestamp":1790766964426,"version":"4.1.0"},"reference-count":16,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T00:00:00Z","timestamp":1677024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation de la Recherche M\u00e9dicale (FRM) Bioinformatics Analysis for Biology Research program","award":["DBI20131228570"],"award-info":[{"award-number":["DBI20131228570"]}]},{"name":"Inserm","award":["DBI20131228570"],"award-info":[{"award-number":["DBI20131228570"]}]},{"name":"CEA","award":["DBI20131228570"],"award-info":[{"award-number":["DBI20131228570"]}]},{"name":"CNRS","award":["DBI20131228570"],"award-info":[{"award-number":["DBI20131228570"]}]},{"name":"University Paris Descartesand the Secular Society (TSS)","award":["DBI20131228570"],"award-info":[{"award-number":["DBI20131228570"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>T cells have the potential to maintain immunological memory and self-tolerance by recognizing antigens from pathogens or tumors. In pathological situations, failure to generate de novo T cells causes immunodeficiency resulting in acute infections and complications. Hematopoietic stem cells (HSC) transplantation constitutes a valuable option to restore proper immune function. However, delayed T cell reconstitution is observed compared to other lineages. To overcome this difficulty, we developed a new approach to identify populations with efficient lymphoid reconstitution properties. To this end, we use a DNA barcoding strategy based on the insertion into a cell chromosome of a lentivirus (LV) carrying a non-coding DNA fragment named barcode (BC). These will segregate through cell divisions and be present in cells\u2019 progeny. The remarkable characteristic of the method is that different cell types can be tracked simultaneously in the same mouse. Thus, we in vivo barcoded LMPP and CLP progenitors to test their ability to reconstitute the lymphoid lineage. Barcoded progenitors were co-grafted in immuno-compromised mice and their fate analyzed by evaluating the BC composition in transplanted mice. The results highlight the predominant role of LMPP progenitors for lymphoid generation and reveal valuable novel insights to be reconsidered in clinical transplantation assays.<\/jats:p>","DOI":"10.3390\/ijms24054368","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T02:57:34Z","timestamp":1677121054000},"page":"4368","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Co-Transplantation of Barcoded Lymphoid-Primed Multipotent (LMPP) and Common Lymphocyte (CLP) Progenitors Reveals a Major Contribution of LMPP to the Lymphoid Lineage"],"prefix":"10.3390","volume":"24","author":[{"given":"Victoria","family":"Michaels","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, CNRS, INSERM, Institut Necker Enfants Malades-INEM, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2203-3722","authenticated-orcid":false,"given":"Smahane","family":"Chalabi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, Centre National de Recherche en G\u00e9nomique Humaine (CNRGH), 91057 Evry, France"},{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, GABI, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1063-2816","authenticated-orcid":false,"given":"Agnes","family":"Legrand","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, CNRS, INSERM, Institut Necker Enfants Malades-INEM, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Renard","sequence":"additional","affiliation":[{"name":"Atomic Energy and Alternative Energies Agency (CEA), Department of Research in Hemato-Immunology (SRHI), Saint-Louis Hospital, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8673-1493","authenticated-orcid":false,"given":"Emmanuel","family":"Tejerina","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, CNRS, INSERM, Institut Necker Enfants Malades-INEM, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4002-9401","authenticated-orcid":false,"given":"Marina","family":"Daouya","sequence":"additional","affiliation":[{"name":"Atomic Energy and Alternative Energies Agency (CEA), Department of Research in Hemato-Immunology (SRHI), Saint-Louis Hospital, 75010 Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9, IRSL, HIPI-UMRS 976, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4145-5488","authenticated-orcid":false,"given":"Sylvie","family":"Fabrega","sequence":"additional","affiliation":[{"name":"SFR Necker\u2014US24\/UAR 3633\/\u2014Structure F\u00e9d\u00e9rative de Recherche Necker Plateformes Vecteurs Viraux et Transfert de G\u00e8nes et Cytom\u00e9trie, Facult\u00e9 de M\u00e9decine de Necker, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Megret","sequence":"additional","affiliation":[{"name":"SFR Necker\u2014US24\/UAR 3633\/\u2014Structure F\u00e9d\u00e9rative de Recherche Necker Plateformes Vecteurs Viraux et Transfert de G\u00e8nes et Cytom\u00e9trie, Facult\u00e9 de M\u00e9decine de Necker, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Olaso","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, Centre National de Recherche en G\u00e9nomique Humaine (CNRGH), 91057 Evry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Boland","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, Centre National de Recherche en G\u00e9nomique Humaine (CNRGH), 91057 Evry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Deleuze","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, Centre National de Recherche en G\u00e9nomique Humaine (CNRGH), 91057 Evry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6849-7824","authenticated-orcid":false,"given":"Christophe","family":"Battail","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, Centre National de Recherche en G\u00e9nomique Humaine (CNRGH), 91057 Evry, France"},{"name":"Universit\u00e9 Grenoble Alpes, IRIG, Laboratoire Biosciences et Bioing\u00e9nierie pour la Sant\u00e9, UA 13 INSERM-CEA-UGA, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Tronik-Le Roux","sequence":"additional","affiliation":[{"name":"Atomic Energy and Alternative Energies Agency (CEA), Department of Research in Hemato-Immunology (SRHI), Saint-Louis Hospital, 75010 Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9, IRSL, HIPI-UMRS 976, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Ezine","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, CNRS, INSERM, Institut Necker Enfants Malades-INEM, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.smim.2008.07.005","article-title":"Delineating the Cellular Pathways of Hematopoietic Lineage Commitment","volume":"20","author":"Luc","year":"2008","journal-title":"Semin. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.stem.2015.05.003","article-title":"Functionally Distinct Subsets of Lineage-Biased Multipotent Progenitors Control Blood Production in Normal and Regenerative Conditions","volume":"17","author":"Pietras","year":"2015","journal-title":"Cell Stem Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/S0092-8674(00)80453-5","article-title":"Identification of Clonogenic Common Lymphoid Progenitors in Mouse Bone Marrow","volume":"91","author":"Kondo","year":"1997","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1002\/stem.1383","article-title":"Arrayed Lentiviral Barcoding for Quantification Analysis of Hematopoietic Dynamics","volume":"31","author":"Grosselin","year":"2013","journal-title":"Stem Cells"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1182\/blood-2013-01-481135","article-title":"Heterogeneity of Young and Aged Murine Hematopoietic Stem Cells Revealed by Quantitative Clonal Analysis Using Cellular Barcoding","volume":"122","author":"Verovskaya","year":"2013","journal-title":"Blood"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1038\/nbt.1977","article-title":"Tracking Single Hematopoietic Stem Cells in Vivo Using High-Throughput Sequencing in Conjunction with Viral Genetic Barcoding","volume":"29","author":"Lu","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/nature12013","article-title":"Diverse and Heritable Lineage Imprinting of Early Haematopoietic Progenitors","volume":"496","author":"Naik","year":"2013","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.immuni.2016.11.004","article-title":"Hematopoietic Stem Cell Niches Produce Lineage-Instructive Signals to Control Multipotent Progenitor Differentiation","volume":"45","author":"Hara","year":"2016","journal-title":"Immunity"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nature11885","article-title":"Haematopoietic Stem Cells and Early Lymphoid Progenitors Occupy Distinct Bone Marrow Niches","volume":"495","author":"Ding","year":"2013","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3258","DOI":"10.1182\/blood-2003-11-4011","article-title":"Hematopoiesis Is Severely Altered in Mice with an Induced Osteoblast Deficiency","volume":"103","author":"Visnjic","year":"2004","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1016\/j.immuni.2016.05.012","article-title":"Sepsis-Induced Osteoblast Ablation Causes Immunodeficiency","volume":"44","author":"Terashima","year":"2016","journal-title":"Immunity"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.celrep.2016.03.039","article-title":"Common-Lymphoid-Progenitor-Independent Pathways of Innate and T Lymphocyte Development","volume":"15","author":"Ghaedi","year":"2016","journal-title":"Cell Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"E6020","DOI":"10.1073\/pnas.1519118112","article-title":"Development of a Diverse Human T-Cell Repertoire despite Stringent Restriction of Hematopoietic Clonality in the Thymus","volume":"112","author":"Brugman","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","unstructured":"Website Available online: Andrews, S. (2016, October 26). FastQC: A Quality Control Tool for High Throughput Sequence Data. Available online: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast Gapped-Read Alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/5\/4368\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:39:34Z","timestamp":1760121574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/5\/4368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,22]]},"references-count":16,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["ijms24054368"],"URL":"https:\/\/doi.org\/10.3390\/ijms24054368","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,22]]}}}