{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:55:36Z","timestamp":1788303336566,"version":"build-2803163510"},"reference-count":27,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T00:00:00Z","timestamp":1677110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Gda\u0144sk under \u201cExcellence Initiative\u2014Research University\u201d program","award":["DEC-2\/2021\/IDUB\/V.6\/Si"],"award-info":[{"award-number":["DEC-2\/2021\/IDUB\/V.6\/Si"]}]},{"name":"SILICIUM SUPPORTING CORE R&amp;D FACILITIES\u2014\u201cExcellence Initiative\u2014Research University\u201d program","award":["DEC-2\/2021\/IDUB\/V.6\/Si"],"award-info":[{"award-number":["DEC-2\/2021\/IDUB\/V.6\/Si"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The untypical course of reaction between chalcones and benzenesulfonylaminoguanidines led to the new 3-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)-2-(1-phenyl-3-arylprop-2-enylideneamino)guanidine derivatives 8\u201333. The new compounds were tested in vitro for their impact on the growth of breast cancer cells MCF-7, cervical cancer cells HeLa and colon cancer cells HCT-116 by MTT assay. The results revealed that the activity of derivatives is strongly related to the presence of hydroxy group in the benzene ring at the 3-arylpropylidene fragment. The most cytotoxic compounds 20 and 24 displayed mean IC50 values of 12.8 and 12.7 \u03bcM, respectively, against three tested cell lines and were almost 3- and 4-fold more active toward MCF-7 and HCT-116 when compared with non-malignant HaCaT cells. Furthermore, compound 24 induced apoptosis in cancer cells and caused a decrease of mitochondrial membrane potential as well as an increase of cells in sub-G1 phase in contrast to its inactive analog 31. The strongest activity against the most sensitive HCT-116 cell line was found for compound 30 (IC50 = 8 \u03bcM), which was 11-fold more effective in the growth inhibition of HCT-116 cells than those of HaCaT cells. Based on this fact, the new derivatives may be promising leading structures for the search for agents for the treatment of colon cancer.<\/jats:p>","DOI":"10.3390\/ijms24054436","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T02:32:12Z","timestamp":1677205932000},"page":"4436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis of 3-(2-Alkylthio-4-chloro-5-methylbenzenesulfonyl)-2-(1-phenyl-3-arylprop-2-enylideneamino)guanidine Derivatives with Pro-Apoptotic Activity against Cancer Cells"],"prefix":"10.3390","volume":"24","author":[{"given":"Aneta","family":"Pogorzelska","sequence":"first","affiliation":[{"name":"Department of Organic Chemistry, Medical University of Gda\u0144sk, Al. Gen. J. Hallera 107, 80-416 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2794-5031","authenticated-orcid":false,"given":"Jaros\u0142aw","family":"S\u0142awi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Medical University of Gda\u0144sk, Al. Gen. J. Hallera 107, 80-416 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8105-2555","authenticated-orcid":false,"given":"Anna","family":"Kawiak","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gda\u0144sk and Medical University of Gda\u0144sk, Abrahama 58, 80-307 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Stasi\u0142oj\u0107","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Immunology, Intercollegiate Faculty of Biotechnology of UG and MUG, Medical University of Gda\u0144sk, D\u0119binki 1, 80-211 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2453-8214","authenticated-orcid":false,"given":"Jaros\u0142aw","family":"Chojnacki","sequence":"additional","affiliation":[{"name":"Department of Inorganic Chemistry, Gda\u0144sk University of Technology, Narutowicza 11\/12, 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,23]]},"reference":[{"key":"ref_1","unstructured":"(2022, June 27). 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