{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:11:02Z","timestamp":1790428262907,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T00:00:00Z","timestamp":1677110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Grant of Excellence Departments, MIUR-Italy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Telomerase reverse transcriptase (TERT) is the catalytic subunit of telomerase holoenzyme, which adds telomeric DNA repeats on chromosome ends to counteract telomere shortening. In addition, there is evidence of TERT non-canonical functions, among which is an antioxidant role. In order to better investigate this role, we tested the response to X-rays and H2O2 treatment in hTERT-overexpressing human fibroblasts (HF-TERT). We observed in HF-TERT a reduced induction of reactive oxygen species and an increased expression of the proteins involved in the antioxidant defense. Therefore, we also tested a possible role of TERT inside mitochondria. We confirmed TERT mitochondrial localization, which increases after oxidative stress (OS) induced by H2O2 treatment. We next evaluated some mitochondrial markers. The basal mitochondria quantity appeared reduced in HF-TERT compared to normal fibroblasts and an additional reduction was observed after OS; nevertheless, the mitochondrial membrane potential and morphology were better conserved in HF-TERT. Our results suggest a protective function of TERT against OS, also preserving mitochondrial functionality.<\/jats:p>","DOI":"10.3390\/ijms24054450","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T02:32:12Z","timestamp":1677205932000},"page":"4450","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["TERT Extra-Telomeric Roles: Antioxidant Activity and Mitochondrial Protection"],"prefix":"10.3390","volume":"24","author":[{"given":"Jessica","family":"Marinaccio","sequence":"first","affiliation":[{"name":"Department of Science, University \u201cROMA TRE\u201d, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1045-2209","authenticated-orcid":false,"given":"Emanuela","family":"Micheli","sequence":"additional","affiliation":[{"name":"Department of Science, University \u201cROMA TRE\u201d, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ion","family":"Udroiu","sequence":"additional","affiliation":[{"name":"Department of Science, University \u201cROMA TRE\u201d, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Di Nottia","sequence":"additional","affiliation":[{"name":"Unit of Muscular and Neurodegenerative Disorders, Laboratory of Molecular Medicine, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3327-4054","authenticated-orcid":false,"given":"Rosalba","family":"Carrozzo","sequence":"additional","affiliation":[{"name":"Unit of Muscular and Neurodegenerative Disorders, Laboratory of Molecular Medicine, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6996-4797","authenticated-orcid":false,"given":"Nicol\u00f2","family":"Baranzini","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9258-7595","authenticated-orcid":false,"given":"Annalisa","family":"Grimaldi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6339-6397","authenticated-orcid":false,"given":"Stefano","family":"Leone","sequence":"additional","affiliation":[{"name":"Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1079-3222","authenticated-orcid":false,"given":"Sandra","family":"Moreno","sequence":"additional","affiliation":[{"name":"Department of Science, University \u201cROMA TRE\u201d, 00146 Rome, Italy"},{"name":"IRCCS Santa Lucia Foundation, 00179 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Muzzi","sequence":"additional","affiliation":[{"name":"Department of Science, University \u201cROMA TRE\u201d, 00146 Rome, Italy"},{"name":"IRCCS Santa Lucia Foundation, 00179 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Sgura","sequence":"additional","affiliation":[{"name":"Department of Science, University \u201cROMA TRE\u201d, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/0092-8674(87)90576-9","article-title":"The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity","volume":"51","author":"Greider","year":"1987","journal-title":"Cell"},{"key":"ref_2","first-page":"1496","article-title":"Principle of marginotomy in template synthesis of polynucleotides","volume":"201","author":"Olovnikov","year":"1971","journal-title":"Dokl. 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