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Challenges in IBC include accurate and early diagnosis and the development of effective targeted therapies. Our previous studies identified the overexpression of metadherin (MTDH) in the plasma membrane of IBC cells, further confirmed in patient tissues. MTDH has been found to play a role in signaling pathways related to cancer. However, its mechanism of action in the progression of IBC remains unknown. To evaluate the function of MTDH, SUM-149 and SUM-190 IBC cells were edited with CRISPR\/Cas9 vectors for in vitro characterization studies and used in mouse IBC xenografts. Our results demonstrate that the absence of MTDH significantly reduces IBC cell migration, proliferation, tumor spheroid formation, and the expression of NF-\u03baB and STAT3 signaling molecules, which are crucial oncogenic pathways in IBC. Furthermore, IBC xenografts showed significant differences in tumor growth patterns, and lung tissue revealed epithelial-like cells in 43% of wild-type (WT) compared to 29% of CRISPR xenografts. Our study emphasizes the role of MTDH as a potential therapeutic target for the progression of IBC.<\/jats:p>","DOI":"10.3390\/ijms24054694","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T02:28:17Z","timestamp":1677637697000},"page":"4694","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Metadherin Regulates Inflammatory Breast Cancer Invasion and Metastasis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2037-5881","authenticated-orcid":false,"given":"Gabriela","family":"Ortiz-Soto","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia S.","family":"Babilonia-D\u00edaz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes Y.","family":"Lacourt-Ventura","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delmarie M.","family":"Rivera-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8375-0645","authenticated-orcid":false,"given":"Jailenne I.","family":"Qui\u00f1ones-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Clinical Anatomy, College of Osteopathic Medicine, Sam Houston State University, Conroe, TX 77304, USA"},{"name":"Department of Anatomy and Cell Biology, School of Medicine, Universidad Central del Caribe, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Col\u00f3n-Vargas","sequence":"additional","affiliation":[{"name":"Department of Mathematical Sciences, University of Puerto Rico at Mayag\u00fcez, Mayag\u00fcez, PR 00681, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-2281","authenticated-orcid":false,"given":"Israel","family":"Almod\u00f3var-Rivera","sequence":"additional","affiliation":[{"name":"Department of Mathematical Sciences, University of Puerto Rico at Mayag\u00fcez, Mayag\u00fcez, PR 00681, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis E.","family":"Ferrer-Torres","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Hospital Interamericano de Medicina Avanzada (H.I.M.A.)\u2014San Pablo Caguas, Caguas, PR 00725, USA"},{"name":"Department of Immunopathology, Hato Rey Pathology Associates Inc. (HRPLABS), San Juan, PR 00936, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5252-9227","authenticated-orcid":false,"given":"Ivette J.","family":"Su\u00e1rez-Arroyo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6933-9905","authenticated-orcid":false,"given":"Michelle M.","family":"Mart\u00ednez-Montemayor","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayam\u00f3n, PR 00960, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3322\/caac.20082","article-title":"Inflammatory breast cancer: The disease, the biology, the treatment","volume":"60","author":"Robertson","year":"2010","journal-title":"CA Cancer J. 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