{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T21:28:40Z","timestamp":1768253320242,"version":"3.49.0"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gastrostart (NVGE)"},{"name":"ChiRhoStim"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Secretin-stimulated pancreatic juice (PJ), collected from the duodenum, presents a valuable biomarker source for the (earlier) detection of pancreatic cancer (PC). Here, we evaluate the feasibility and performance of shallow sequencing to detect copy number variations (CNVs) in cell-free DNA (cfDNA) from PJ for PC detection. First, we confirmed the feasibility of shallow sequencing in PJ (n = 4), matched plasma (n = 3) and tissue samples (n = 4, microarray). Subsequently, shallow sequencing was performed on cfDNA from PJ of 26 cases (25 sporadic PC, 1 high-grade dysplasia) and 19 controls with a hereditary or familial increased risk of PC. 40 of the 45 PJ samples met the quality criteria for cfDNA analysis. Nine individuals had an 8q24 gain (oncogene MYC; 23%; eight cases (33%) and one control (6%), p = 0.04); six had both a 2q gain (STAT1) and 5p loss (CDH10; 15%; four cases (7%) and two controls (13%), p = 0.72). The presence of an 8q24 gain differentiated the cases and controls, with a sensitivity of 33% (95% CI 16\u201355%) and specificity of 94% (95% CI 70\u2013100%). The presence of either an 8q24 or 2q gain with a 5p loss was related to a sensitivity of 50% (95% CI 29\u201371%) and specificity of 81% (95% CI 54\u201396%). Shallow sequencing of PJ is feasible. The presence of an 8q24 gain in PJ shows promise as a biomarker for the detection of PC. Further research is required with a larger sample size and consecutively collected samples in high-risk individuals prior to implementation in a surveillance cohort.<\/jats:p>","DOI":"10.3390\/ijms24065097","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T02:30:23Z","timestamp":1678242623000},"page":"5097","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An 8q24 Gain in Pancreatic Juice Is a Candidate Biomarker for the Detection of Pancreatic Cancer"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9825-8728","authenticated-orcid":false,"given":"Iris J. M.","family":"Levink","sequence":"first","affiliation":[{"name":"Department of Gastroenterology & Hepatology, Erasmus MC, University Medical Center, 3015 GD Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3429-6156","authenticated-orcid":false,"given":"Malgorzata I.","family":"Srebniak","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC, University Medical Center, 3015 CN Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter G.","family":"De Valk","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC, University Medical Center, 3015 CN Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique M.","family":"van Veghel-Plandsoen","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC, University Medical Center, 3015 CN Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC, University Medical Center, 3015 CN Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Djuna L.","family":"Cahen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology & Hepatology, Erasmus MC, University Medical Center, 3015 GD Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9221-4855","authenticated-orcid":false,"given":"Gwenny M.","family":"Fuhler","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology & Hepatology, Erasmus MC, University Medical Center, 3015 GD Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco J.","family":"Bruno","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology & Hepatology, Erasmus MC, University Medical Center, 3015 GD Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1136\/gutjnl-2020-323611","article-title":"Long-term yield of pancreatic cancer surveillance in high-risk individuals","volume":"71","author":"Overbeek","year":"2022","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"103716","DOI":"10.1016\/j.ebiom.2021.103716","article-title":"Identification of copy number variation-driven molecular subtypes informative for prognosis and treatment in pancreatic adenocarcinoma of a Chinese cohort","volume":"74","author":"Zhan","year":"2021","journal-title":"eBioMedicine"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/s41416-020-01047-5","article-title":"Clinical relevance of blood-based ctDNA analysis: Mutation detection and beyond","volume":"124","author":"Keller","year":"2021","journal-title":"Br. J. Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1038\/s41698-022-00306-3","article-title":"Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors","volume":"6","author":"Bonner","year":"2022","journal-title":"npj Precis. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1001\/jamaoncol.2015.1883","article-title":"Presymptomatic Identification of Cancers in Pregnant Women During Noninvasive Prenatal Testing","volume":"1","author":"Amant","year":"2015","journal-title":"JAMA Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1001\/jama.2015.7120","article-title":"Noninvasive Prenatal Testing and Incidental Detection of Occult Maternal Malignancies","volume":"314","author":"Bianchi","year":"2015","journal-title":"JAMA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e6280","DOI":"10.1002\/ccr3.6280","article-title":"Incidental finding of maternal malignancy in an unusual non-invasive prenatal test and a review of similar cases","volume":"10","author":"Moellgaard","year":"2022","journal-title":"Clin. Case Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1200\/JCO.21.02260","article-title":"Noninvasive Prenatal Test Results Indicative of Maternal Malignancies: A Nationwide Genetic and Clinical Follow-Up Study","volume":"40","author":"Heesterbeek","year":"2022","journal-title":"J. Clin. Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"16022","DOI":"10.1038\/nrdp.2016.22","article-title":"Pancreatic cancer","volume":"2","author":"Kleeff","year":"2016","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"v56","DOI":"10.1093\/annonc\/mdv295","article-title":"Cancer of the pancreas: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up","volume":"26","author":"Ducreux","year":"2015","journal-title":"Ann. Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.14309\/ajg.0000000000000939","article-title":"Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer","volume":"115","author":"Levink","year":"2020","journal-title":"Am. J. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4267","DOI":"10.1093\/nar\/9.17.4267","article-title":"The helical periodicity of DNA on the nucleosome","volume":"9","author":"Klug","year":"1981","journal-title":"Nucleic Acids Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.ajhg.2020.01.008","article-title":"The Biology of Cell-free DNA Fragmentation and the Roles of DNASE1, DNASE1L3, and DFFB","volume":"106","author":"Han","year":"2020","journal-title":"Am. J. Hum. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1038\/modpathol.3880547","article-title":"c-MYC Activation in Primary and Metastatic Ductal Adenocarcinoma of the Pancreas: Incidence, Mechanisms, and Clinical Significance","volume":"15","author":"Schleger","year":"2002","journal-title":"Mod. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"696","DOI":"10.7150\/jca.10094","article-title":"Circulating microRNAs in Pancreatic Juice as Candidate Biomarkers of Pancreatic Cancer","volume":"5","author":"Wang","year":"2014","journal-title":"J. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8908","DOI":"10.1093\/nar\/gky658","article-title":"PARP10 promotes cellular proliferation and tumorigenesis by alleviating replication stress","volume":"46","author":"Schleicher","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1053\/j.gastro.2021.12.281","article-title":"Off-the-Shelf Prostate Stem Cell Antigen\u2013Directed Chimeric Antigen Receptor Natural Killer Cell Therapy to Treat Pancreatic Cancer","volume":"162","author":"Teng","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1158\/1078-0432.CCR-10-0999","article-title":"Plectin-1 as a novel biomarker for pancreatic cancer","volume":"17","author":"Bausch","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1039\/d0mt00227e","article-title":"Plecstatin-1 induces an immunogenic cell death signature in colorectal tumour spheroids","volume":"12","author":"Wernitznig","year":"2020","journal-title":"Metallomics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13046-017-0537-x","article-title":"Heat shock factor 1 inhibits the mitochondrial apoptosis pathway by regulating second mitochondria-derived activator of caspase to promote pancreatic tumorigenesis","volume":"36","author":"Liang","year":"2017","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"515","DOI":"10.2174\/1568009618666181018162117","article-title":"HSF1 as a Cancer Biomarker and Therapeutic Target","volume":"19","author":"Carpenter","year":"2019","journal-title":"Curr. Cancer Drug Targets"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1002\/1878-0261.12314","article-title":"Induced PTF1a expression in pancreatic ductal adenocarcinoma cells activates acinar gene networks, reduces tumorigenic properties, and sensitizes cells to gemcitabine treatment","volume":"12","author":"Jakubison","year":"2018","journal-title":"Mol. Oncol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1134\/S1607672917040020","article-title":"Expression of master regulatory genes of embryonic development in pancreatic tumors","volume":"475","author":"Kondratyeva","year":"2017","journal-title":"Dokl. Biochem. Biophys."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e07125","DOI":"10.7554\/eLife.07125","article-title":"The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma","volume":"4","author":"Krah","year":"2015","journal-title":"eLife"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1042\/BJ20120873","article-title":"ICAT is a novel Ptf1a interactor that regulates pancreatic acinar differentiation and displays altered expression in tumours","volume":"451","author":"Campos","year":"2013","journal-title":"Biochem. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7101","DOI":"10.1073\/pnas.0902508106","article-title":"Bmi1 lineage tracing identifies a self-renewing pancreatic acinar cell subpopulation capable of maintaining pancreatic organ homeostasis","volume":"106","author":"Sangiorgi","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1053\/j.gastro.2012.05.009","article-title":"Bmi1 is required for regeneration of the exocrine pancreas in mice","volume":"143","author":"Fukuda","year":"2012","journal-title":"Gastroenterology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1101\/gad.1742609","article-title":"Bmi-1 regulates the Ink4a\/Arf locus to control pancreatic beta-cell proliferation","volume":"23","author":"Dhawan","year":"2009","journal-title":"Genes Dev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1093\/carcin\/bgv058","article-title":"Bmi1 is required for the initiation of pancreatic cancer through an Ink4a-independent mechanism","volume":"36","author":"Bednar","year":"2015","journal-title":"Carcinogenesis"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/nature10189","article-title":"Oncogene-induced Nrf2 transcription promotes ROS detoxification and tumorigenesis","volume":"475","author":"DeNicola","year":"2011","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0925-4439(00)00054-5","article-title":"The 18q21 region in colorectal and pancreatic cancer: Independent loss of DCC and DPC4 expression","volume":"1502","author":"Carrato","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1111\/his.13894","article-title":"Loss of SMAD4 protein expression in gastrointestinal and extra-gastrointestinal carcinomas","volume":"75","author":"Ritterhouse","year":"2019","journal-title":"Histopathology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"16704","DOI":"10.18632\/oncotarget.14335","article-title":"Clinicopathological significance of SMAD4 loss in pancreatic ductal adenocarcinomas: A systematic review and meta-analysis","volume":"8","author":"Wang","year":"2017","journal-title":"Oncotarget"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1038\/sj.cdd.4401962","article-title":"The p53-Bcl-2 connection","volume":"13","author":"Hemann","year":"2006","journal-title":"Cell Death Differ."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nature14169","article-title":"Whole genomes redefine the mutational landscape of pancreatic cancer","volume":"518","author":"Waddell","year":"2015","journal-title":"Nature"},{"key":"ref_36","first-page":"7849","article-title":"Expression of Beclin 1 and Bcl-2 in pancreatic neoplasms and its effect on pancreatic ductal adenocarcinoma prognosis","volume":"14","author":"Song","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1159\/000055820","article-title":"Detailed tissue expression of bcl-2, bax, bak and bcl-x in the normal human pancreas and in chronic pancreatitis, ampullary and pancreatic ductal adenocarcinomas","volume":"1","author":"Evans","year":"2001","journal-title":"Pancreatology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"24719","DOI":"10.18632\/oncotarget.8348","article-title":"Netrin-1 suppresses the MEK\/ERK pathway and ITGB4 in pancreatic cancer","volume":"7","author":"An","year":"2016","journal-title":"Oncotarget"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"840","DOI":"10.3748\/wjg.v18.i8.840","article-title":"Expression of fibroblast activation protein in human pancreatic adenocarcinoma and its clinicopathological significance","volume":"18","author":"Shi","year":"2012","journal-title":"World J. Gastroenterol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e92232","DOI":"10.1172\/jci.insight.92232","article-title":"Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma","volume":"2","author":"Lo","year":"2017","journal-title":"JCI Insight"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lee, H.-O., Mullins, S.R., Franco-Barraza, J., Valianou, M., Cukierman, E., and Cheng, J.D. (2011). FAP-overexpressing fibroblasts produce an extracellular matrix that enhances invasive velocity and directionality of pancreatic cancer cells. BMC Cancer, 11.","DOI":"10.1186\/1471-2407-11-245"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.cyto.2015.11.011","article-title":"The good and the bad faces of STAT1 in solid tumours","volume":"89","author":"Meissl","year":"2017","journal-title":"Cytokine"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1758835920917558","DOI":"10.1177\/1758835920917558","article-title":"Predicting STAT1 as a prognostic marker in patients with solid cancer","volume":"12","author":"Zhang","year":"2020","journal-title":"Ther. Adv. Med. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1097\/MPA.0000000000000074","article-title":"Differential expression of STAT1 and p21 proteins predicts pancreatic cancer progression and prognosis","volume":"43","author":"Sun","year":"2014","journal-title":"Pancreas"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1002\/gcc.22447","article-title":"Alterations of type II classical cadherin, cadherin-10 (CDH10), is associated with pancreatic ductal adenocarcinomas","volume":"56","author":"Jinawath","year":"2017","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1038\/s41416-020-0782-1","article-title":"Targeting the HGF\/c-MET pathway in advanced pancreatic cancer: A key element of treatment that limits primary tumour growth and eliminates metastasis","volume":"122","author":"Xu","year":"2020","journal-title":"Br. J. Cancer"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/bjc.2015.478","article-title":"Hepatocyte growth factor inhibition: A novel therapeutic approach in pancreatic cancer","volume":"114","author":"Pothula","year":"2016","journal-title":"Br. J. Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1038\/s41388-022-02302-0","article-title":"Suppression of mutant Kirsten-RAS (KRASG12D)-driven pancreatic carcinogenesis by dual-specificity MAP kinase phosphatases 5 and 6","volume":"41","author":"Kidger","year":"2022","journal-title":"Oncogene"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/s41388-021-02140-6","article-title":"FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression","volume":"41","author":"Guo","year":"2022","journal-title":"Oncogene"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1007\/s11605-008-0577-9","article-title":"Loss of heterozygosity predicts poor survival after resection of pancreatic adenocarcinoma","volume":"12","author":"Franko","year":"2008","journal-title":"J. Gastrointest. Surg."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"28558","DOI":"10.18632\/oncotarget.15331","article-title":"Genetic characterization of Polish ccRCC patients: Somatic mutation analysis of PBRM1, BAP1 and KDMC5, genomic SNP array analysis in tumor biopsy and preliminary results of chromosome aberrations analysis in plasma cell free DNA","volume":"8","author":"Kluzek","year":"2017","journal-title":"Oncotarget"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"17562848211014818","DOI":"10.1177\/17562848211014818","article-title":"The role of PARP inhibitors in BRCA mutated pancreatic cancer","volume":"14","author":"Chi","year":"2021","journal-title":"Therap. Adv. Gastroenterol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"17588359221083050","DOI":"10.1177\/17588359221083050","article-title":"Platinum-based chemotherapy for pancreatic cancer: Impact of mutations in the homologous recombination repair and Fanconi anemia genes","volume":"14","author":"Emelyanova","year":"2022","journal-title":"Ther. Adv. Med. Oncol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s12957-015-0451-7","article-title":"pERK1\/2 silencing sensitizes pancreatic cancer BXPC-3 cell to gemcitabine-induced apoptosis via regulating Bax and Bcl-2 expression","volume":"13","author":"Wang","year":"2015","journal-title":"World J. Surg. Oncol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"eaat4921","DOI":"10.1126\/scitranslmed.aat4921","article-title":"Enhanced detection of circulating tumor DNA by fragment size analysis","volume":"10","author":"Mouliere","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Underhill, H.R., Kitzman, J.O., Hellwig, S., Welker, N.C., Daza, R., Baker, D.N., Gligorich, K.M., Rostomily, R.C., Bronner, M.P., and Shendure, J. (2016). Fragment Length of Circulating Tumor DNA. PLoS Genet., 12.","DOI":"10.1371\/journal.pgen.1006162"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"36097","DOI":"10.1038\/srep36097","article-title":"Very Short Mitochondrial DNA Fragments and Heteroplasmy in Human Plasma","volume":"6","author":"Zhang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1158\/0008-5472.CAN-18-0074","article-title":"Measurement of Plasma Cell-Free Mitochondrial Tumor DNA Improves Detection of Glioblastoma in Patient-Derived Orthotopic Xenograft Models","volume":"79","author":"Mair","year":"2019","journal-title":"Cancer Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"6744","DOI":"10.1038\/ncomms7744","article-title":"Whole-exome sequencing of pancreatic cancer defines genetic diversity and therapeutic targets","volume":"6","author":"Witkiewicz","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1016\/j.ajpath.2018.03.012","article-title":"Genome-Wide Somatic Copy Number Alterations and Mutations in High-Grade Pancreatic Intraepithelial Neoplasia","volume":"188","author":"Hata","year":"2018","journal-title":"Am. J. Pathol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/(SICI)1096-9896(200005)191:1<27::AID-PATH582>3.0.CO;2-J","article-title":"Identification of frequent chromosomal aberrations in ductal adenocarcinoma of the pancreas by comparative genomic hybridization (CGH)","volume":"191","author":"Schleger","year":"2000","journal-title":"J. Pathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.1002\/cam4.2340","article-title":"Genomic analysis of pancreatic juice DNA assesses malignant risk of intraductal papillary mucinous neoplasm of pancreas","volume":"8","author":"Mateos","year":"2019","journal-title":"Cancer Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1002\/pd.4615","article-title":"Determination of fetal DNA fraction from the plasma of pregnant women using sequence read counts","volume":"35","author":"Kim","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1093\/nar\/gkt992","article-title":"WISECONDOR: Detection of fetal aberrations from shallow sequencing maternal plasma based on a within-sample comparison scheme","volume":"42","author":"Straver","year":"2014","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/6\/5097\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:49:50Z","timestamp":1760122190000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/6\/5097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,7]]},"references-count":64,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["ijms24065097"],"URL":"https:\/\/doi.org\/10.3390\/ijms24065097","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,7]]}}}