{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:46:12Z","timestamp":1790707572384,"version":"4.1.0"},"reference-count":31,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The risk of toxicity attributable to radioiodine therapy (RIT) remains a subject of ongoing research, with a whole-body dose of 2 Gy proposed as a safe limit. This article evaluates the RIT-induced cytogenetic damage in two rare differentiated thyroid cancer (DTC) cases, including the first follow-up study of a pediatric DTC patient. Chromosome damage in the patient\u2019s peripheral blood lymphocytes (PBL) was examined using conventional metaphase assay, painting of chromosomes 2, 4, and 12 (FISH), and multiplex fluorescence in situ hybridization (mFISH). Patient 1 (female, 1.6 y.o.) received four RIT courses over 1.1 years. Patient 2 (female, 49 y.o.) received 12 courses over 6.4 years, the last two of which were examined. Blood samples were collected before and 3\u20134 days after the treatment. Chromosome aberrations (CA) analyzed by conventional and FISH methods were converted to a whole-body dose accounting for the dose rate effect. The mFISH method showed an increase in total aberrant cell frequency following each RIT course, while cells carrying unstable aberrations predominated in the yield. The proportion of cells containing stable CA associated with long-term cytogenetic risk remained mostly unchanged during follow-up for both patients. A one-time administration of RIT was safe, as the threshold of 2 Gy for the whole-body dose was not exceeded. The risk of side effects projected from RIT-attributable cytogenetic damage was low, suggesting a good long-term prognosis. In rare cases, such as the ones reviewed in this study, individual planning based on cytogenetic biodosimetry is strongly recommended.<\/jats:p>","DOI":"10.3390\/ijms24065128","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T02:30:23Z","timestamp":1678242623000},"page":"5128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study"],"prefix":"10.3390","volume":"24","author":[{"given":"Igor K.","family":"Khvostunov","sequence":"first","affiliation":[{"name":"A.F. Tsyb Medical Radiological Research Center (MRRC)\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroliova St., 249036 Obninsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8145-7472","authenticated-orcid":false,"given":"Elena","family":"Nasonova","sequence":"additional","affiliation":[{"name":"Joint Institute for Nuclear Research (JINR), 6 Joliot-Curie St., 141980 Dubna, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeriy","family":"Krylov","sequence":"additional","affiliation":[{"name":"A.F. Tsyb Medical Radiological Research Center (MRRC)\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroliova St., 249036 Obninsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Rodichev","sequence":"additional","affiliation":[{"name":"A.F. Tsyb Medical Radiological Research Center (MRRC)\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroliova St., 249036 Obninsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Kochetova","sequence":"additional","affiliation":[{"name":"A.F. Tsyb Medical Radiological Research Center (MRRC)\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroliova St., 249036 Obninsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Shepel","sequence":"additional","affiliation":[{"name":"A.F. Tsyb Medical Radiological Research Center (MRRC)\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroliova St., 249036 Obninsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Korovchuk","sequence":"additional","affiliation":[{"name":"A.F. Tsyb Medical Radiological Research Center (MRRC)\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroliova St., 249036 Obninsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Kutsalo","sequence":"additional","affiliation":[{"name":"Joint Institute for Nuclear Research (JINR), 6 Joliot-Curie St., 141980 Dubna, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Shegai","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution, National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 2 Botkinskiy Proezd, 125284 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Kaprin","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution, National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 2 Botkinskiy Proezd, 125284 Moscow, Russia"},{"name":"Federal State Autonomous Educational Institution of Higher Professional Education, Department of Oncology and Radiology Named after N.P. Kharchenko, Medical Institute, Peoples\u2019 Friendship University of Russia, 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1631\/jzus.B1400131","article-title":"Therapeutic radionuclides in nuclear medicine: Current and future prospects. Review","volume":"15","author":"Yeong","year":"2014","journal-title":"J. Zhejiang Univ.-Sci. B (Biomed. 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