{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:47:42Z","timestamp":1781200062280,"version":"3.54.1"},"reference-count":94,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council (ERC)","doi-asserted-by":"publisher","award":["852814"],"award-info":[{"award-number":["852814"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The adult human heart cannot regain complete cardiac function following tissue injury, making cardiac regeneration a current clinical unmet need. There are a number of clinical procedures aimed at reducing ischemic damage following injury; however, it has not yet been possible to stimulate adult cardiomyocytes to recover and proliferate. The emergence of pluripotent stem cell technologies and 3D culture systems has revolutionized the field. Specifically, 3D culture systems have enhanced precision medicine through obtaining a more accurate human microenvironmental condition to model disease and\/or drug interactions in vitro. In this study, we cover current advances and limitations in stem cell-based cardiac regenerative medicine. Specifically, we discuss the clinical implementation and limitations of stem cell-based technologies and ongoing clinical trials. We then address the advent of 3D culture systems to produce cardiac organoids that may better represent the human heart microenvironment for disease modeling and genetic screening. Finally, we delve into the insights gained from cardiac organoids in relation to cardiac regeneration and further discuss the implications for clinical translation.<\/jats:p>","DOI":"10.3390\/ijms24065188","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T02:33:16Z","timestamp":1678329196000},"page":"5188","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Advances in 3D Organoid Models for Stem Cell-Based Cardiac Regeneration"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0035-0185","authenticated-orcid":false,"given":"Marcy","family":"Martin","sequence":"first","affiliation":[{"name":"Institute for Regenerative Medicine (IREM), University of Zurich, 8952 Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric K. N.","family":"G\u00e4hwiler","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine (IREM), University of Zurich, 8952 Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Generali","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine (IREM), University of Zurich, 8952 Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon P.","family":"Hoerstrup","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine (IREM), University of Zurich, 8952 Schlieren, Switzerland"},{"name":"Wyss Zurich Translational Center, University of Zurich and ETH Zurich, 8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian Y.","family":"Emmert","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine (IREM), University of Zurich, 8952 Schlieren, Switzerland"},{"name":"Wyss Zurich Translational Center, University of Zurich and ETH Zurich, 8092 Zurich, Switzerland"},{"name":"Department of Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum der Charit\u00e9 (DHZC), 13353 Berlin, Germany"},{"name":"Department of Cardiovascular Surgery, Charit\u00e9 Universit\u00e4tsmedizin Berlin, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022, December 20). 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