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When the planktonic larvae are fully developed, they must find a favorable site to settle and metamorphose into benthic juveniles. This transition from a planktonic to a benthic mode of life is a complex behavioral process involving substrate searching and exploration. Although the mechanosensitive receptor in the tactile sensor has been implicated in sensing and responding to surfaces of the substrates, few have been unambiguously identified. Recently, we identified that the mechanosensitive transient receptor potential melastatin-subfamily member 7 (TRPM7) channel, highly expressed in the larval foot of the mussel Mytilospsis sallei, was involved in substrate exploration for settlement. Here, we show that the TRPM7-mediated Ca2+ signal was involved in triggering the larval settlement of M. sallei through the calmodulin-dependent protein kinase kinase \u03b2\/AMP-activated protein kinase\/silk gland factor 1 (CaMKK\u03b2-AMPK-SGF1) pathway. It was found that M. sallei larvae preferred the stiff surfaces for settlement, on which TRPM7, CaMKK\u03b2, AMPK, and SGF1 were highly expressed. These findings will help us to better understand the molecular mechanisms of larval settlement in marine invertebrates, and will provide insights into the potential targets for developing environmentally friendly antifouling coatings for fouling organisms.<\/jats:p>","DOI":"10.3390\/ijms24065399","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T05:36:21Z","timestamp":1678685781000},"page":"5399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["TRPM7-Mediated Ca2+ Regulates Mussel Settlement through the CaMKK\u03b2-AMPK-SGF1 Pathway"],"prefix":"10.3390","volume":"24","author":[{"given":"Jian","family":"He","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Environmental Corrosion and Bio-Fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Environmental Corrosion and Bio-Fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China"},{"name":"Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danqing","family":"Feng","sequence":"additional","affiliation":[{"name":"State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Environmental Corrosion and Bio-Fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,11]]},"reference":[{"key":"ref_1","first-page":"679","article-title":"Ecological regulation of development: Induction of marine invertebrate metamorphosis","volume":"46","author":"Jackson","year":"2004","journal-title":"Int. J. Dev. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"901","DOI":"10.2307\/1313294","article-title":"Metamorphosis is not a new beginning: Larval experience influences juvenile performance","volume":"48","author":"Pechenik","year":"1998","journal-title":"Bioscience"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev.marine.010908.163757","article-title":"Larval dispersal and marine population connectivity","volume":"1","author":"Cowen","year":"2009","journal-title":"Annu. Rev. Mar. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4623","DOI":"10.3390\/ijms10114623","article-title":"Challenges for the development of new non-toxic antifouling solutions","volume":"10","author":"Marechal","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1080\/0892701021000034373","article-title":"Aquatic biofouling larvae respond to differences in the mechanical properties of the surface on which they settle","volume":"18","author":"Gray","year":"2002","journal-title":"Biofouling"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Rosenhahn, A., and Sendra, G.H. (2012). Surface sensing and settlement strategies of marine biofouling organisms. Biointerphases, 7.","DOI":"10.1007\/s13758-012-0063-5"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev-neuro-070918-050509","article-title":"Mechanosensitive ion channels: Structural features relevant to mechanotransduction mechanisms","volume":"43","author":"Jin","year":"2020","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"18566","DOI":"10.1021\/acsnano.1c09097","article-title":"Counterplotting the mechanosensing-based gouling mechanism of mussels against fouling","volume":"15","author":"Choi","year":"2021","journal-title":"ACS Nano"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1126\/science.aai8977","article-title":"Preventing mussel adhesion using lubricant-infused materials","volume":"357","author":"Amini","year":"2017","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Abramova, A., Rosenblad, M.A., Blomberg, A., and Larsson, T.A. (2019). Sensory receptor repertoire in cyprid antennules of the barnacle Balanus improvisus. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0216294"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ecolecon.2004.10.002","article-title":"Update on the environmental and economic costs associated with alien-invasive species in the United States","volume":"52","author":"Pimentel","year":"2005","journal-title":"Ecol. Econ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/543488a","article-title":"Environmental sciences: Troubled waters on the Great Lakes","volume":"543","author":"Michalak","year":"2017","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"923","DOI":"10.2983\/035.030.0334","article-title":"Contrasting rates of spread of two congeners, Dreissena polymorpha and Dreissena rostriformis bugensis, at different spatial scales","volume":"30","author":"Karatayev","year":"2011","journal-title":"J. Shellfish. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10452-010-9344-6","article-title":"The invasive dark falsemussel Mytilopsis leucophaeata (Bivalvia: Dreissenidae): A literature review","volume":"45","author":"Kennedy","year":"2011","journal-title":"Aquat. Ecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10530-010-9825-5","article-title":"High haplotype variability in established Asian populations of the invasive Carib-bean bivalve Mytilopsis sallei (Dreissenidae)","volume":"13","author":"Wong","year":"2011","journal-title":"Biol. Invasions"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"133","DOI":"10.29037\/ajstd.483","article-title":"The invasive Caribbean Mytilopsis sallei (Bivalvia: Dreissenidae): A short review","volume":"35","author":"Tan","year":"2018","journal-title":"ASEAN J. Sci. Technol. Dev."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cao, Y., Zhang, M., Liang, X., Li, F., Shi, Y., Yang, X., and Jiang, C. (2020). Natural variation of an EF-hand Ca2+-binding-protein coding gene confers saline-alkaline tolerance in maize. Nat. Commun., 11.","DOI":"10.1038\/s41467-019-14027-y"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/s41558-020-0776-2","article-title":"Decreased motility of flagellated microalgae long-term acclimated to CO2-induced acidified waters","volume":"10","author":"Wang","year":"2020","journal-title":"Nat. Clim. Chang."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Feng, K., Ni, C., Yu, L., Zhou, W., and Li, X. (2019). Synthesis and antifouling evaluation of indole derivatives. Ecotoxicol. Environ. Saf., 182.","DOI":"10.1016\/j.ecoenv.2019.109423"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1021\/acschembio.1c00175","article-title":"Adenosine triggers larval settlement and metamorphosis in the mussel Mytilopsis sallei through the ADK-AMPK-FoxO pathway","volume":"16","author":"He","year":"2021","journal-title":"ACS Chem. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1084\/jem.20052469","article-title":"Regulation of the energy sensor AMP-activated protein kinase by antigen receptor and Ca2+ in T lymphocytes","volume":"203","author":"Hawley","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s00338-021-02216-5","article-title":"Promoting larval settlement of coral Pocillopora damicornis by calcium","volume":"41","author":"Yang","year":"2022","journal-title":"Coral Reefs"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1017\/S0025315499001484","article-title":"Induction of larval metamorphosis in the ascidian, Halocynthia roretzi by excess potassium ion and by reduced calcium ion","volume":"79","author":"Matsumura","year":"1999","journal-title":"J. Mar. Bio. Assoc. UK"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/08927019609386276","article-title":"Signal transduction in barnacle settlement: Calcium re-visited","volume":"10","author":"Clare","year":"1996","journal-title":"Biofouling"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/j.1749-7345.2010.00403.x","article-title":"The Effect of chemical cues onlarval settlement of the abalone, Haliotis diversicolor supertexta","volume":"41","author":"Yu","year":"2010","journal-title":"J. World Aquac. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.cbpb.2012.04.001","article-title":"Characterization and expression of calmodulin gene during larval settlement and metamorphosis of the polychaete Hydroides elegans","volume":"162","author":"Chen","year":"2012","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.margen.2009.07.002","article-title":"Gene expression microarray analysis encompassing metamorphosis and the onset of calcification in the scleractinian coral Montastraea faveolata","volume":"2","author":"DeSalvo","year":"2009","journal-title":"Mar. Genom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"37546","DOI":"10.1038\/srep37546","article-title":"An ancient role for nitric oxide in regulating the animal pelagobenthic life cycle: Evidence from a marine sponge","volume":"6","author":"Ueda","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhang, W., Wang, Y., Hu, X., Zhou, Z., Zhu, Y., Liang, X., and Yang, J.-L. (2022). AMPK promotes larval metamorphosis of Mytilus coruscus. Genes, 13.","DOI":"10.3390\/genes13122384"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3354\/meps13919","article-title":"Transcriptome analysis provides insights into a molecular mechanism of histamine response in the cyprid larvae of Amphibalanus amphitrite","volume":"681","author":"Jiang","year":"2022","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.tcb.2015.10.013","article-title":"AMPK: An energy-sensing pathway with multiple inputs and outputs","volume":"26","author":"Hardie","year":"2016","journal-title":"Trends Cell Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.molcel.2017.05.032","article-title":"AMPK: Mechanisms of cellular energy sensing and restoration of metabolic balance","volume":"66","author":"Garcia","year":"2017","journal-title":"Mol. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chen, Z.-F., Zhang, H., Wang, H., Matsumura, K., Wong, Y.H., Ravasi, T., and Qian, P.-Y. (2014). Quantitative proteomics study of larval settlement in the Barnacle Balanus amphitrite. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0088744"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1021\/pr3010088","article-title":"Transcriptome and quantitative proteome analysis reveals molecular processes associated with larval metamorphosis in the polychaete Pseudopolydora vexillosa","volume":"12","author":"Chandramouli","year":"2013","journal-title":"J. Proteome Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/nrc3539","article-title":"Forkhead box proteins: Tuning forks for transcriptional harmony","volume":"13","author":"Lam","year":"2013","journal-title":"Nat. Rev. Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/S0965-1748(01)00170-9","article-title":"Fork head alternative binding drives stage-specific gene expression in the silk gland of Bombyx mori","volume":"32","author":"Julien","year":"2002","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.bbamcr.2018.01.007","article-title":"Ras-Raf-MAPK signaling promotes nuclear localization of FOXA transcription factor SGF1 via Ser91 phosphorylation","volume":"1865","author":"Ma","year":"2018","journal-title":"Biochim. Biophys. Acta (BBA) Mol. Cell Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"McCartney, M.A. (2021). Structure, function and parallel evolution of the bivalve byssus, with insights from proteomes and the zebra mussel genome. Philos. Trans. R. Soc. B Biol. Sci., 376.","DOI":"10.1098\/rstb.2020.0155"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"116","DOI":"10.2307\/1542757","article-title":"Catecholamine-containing cells in larval and postlarval bivalve molluscs","volume":"193","author":"Croll","year":"1997","journal-title":"Biol. Bull."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.aquaculture.2018.01.002","article-title":"Molluscan bivalve settlement and metamorphosis: Neuroendocrine inducers and morphogenetic responses","volume":"487","author":"Joyce","year":"2018","journal-title":"Aquaculture"},{"key":"ref_41","first-page":"23","article-title":"Embryonic and larval development of the invasive biofouler Mytilopsis sallei (R\u00e9cluz, 1849) (Bivalvia: Dreissenidae)","volume":"82","author":"He","year":"2016","journal-title":"J. Mollus. Stud."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/6\/5399\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:52:59Z","timestamp":1760122379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/6\/5399"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,11]]},"references-count":42,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["ijms24065399"],"URL":"https:\/\/doi.org\/10.3390\/ijms24065399","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,11]]}}}