{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T03:56:39Z","timestamp":1777089399005,"version":"3.51.4"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,12]],"date-time":"2023-03-12T00:00:00Z","timestamp":1678579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2021-683"],"award-info":[{"award-number":["075-15-2021-683"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["FMFU-2021-0008"],"award-info":[{"award-number":["FMFU-2021-0008"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["94031363"],"award-info":[{"award-number":["94031363"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004285","name":"St. Petersburg State University","doi-asserted-by":"publisher","award":["075-15-2021-683"],"award-info":[{"award-number":["075-15-2021-683"]}],"id":[{"id":"10.13039\/501100004285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004285","name":"St. Petersburg State University","doi-asserted-by":"publisher","award":["FMFU-2021-0008"],"award-info":[{"award-number":["FMFU-2021-0008"]}],"id":[{"id":"10.13039\/501100004285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004285","name":"St. Petersburg State University","doi-asserted-by":"publisher","award":["94031363"],"award-info":[{"award-number":["94031363"]}],"id":[{"id":"10.13039\/501100004285","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ocular surface reconstruction is essential for treating corneal epithelial defects and vision recovery. Stem cell-based therapy demonstrates promising results but requires further research to elucidate stem cell survival, growth, and differentiation after transplantation in vivo. This study examined the corneal reconstruction promoted by EGFP-labeled limbal mesenchymal stem cells (L-MSCs-EGFP) and their fate after transplantation. EGFP labeling allowed us to evaluate the migration and survival rates of the transferred cells. L-MSCs-EGFP seeded onto decellularized human amniotic membrane (dHAM) were transplanted into rabbits with a modeled limbal stem cell deficiency. The localization and viability of the transplanted cells in animal tissue were analyzed using histology, immunohistochemistry, and confocal microscopy up to 3 months after transplantation. EGFP-labeled cells remained viable for the first 14 days after transplantation. By the 90th day, epithelialization of the rabbit corneas reached 90%, but the presence of viable labeled cells was not observed within the newly formed epithelium. Although labeled cells demonstrated low survivability in host tissue, the squamous corneal-like epithelium was partially restored by the 30th day after transplantation of the tissue-engineered graft. Overall, this study paves the way for further optimization of transplantation conditions and studying the mechanisms of corneal tissue restoration.<\/jats:p>","DOI":"10.3390\/ijms24065431","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T05:36:21Z","timestamp":1678685781000},"page":"5431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5257-8408","authenticated-orcid":false,"given":"Julia I.","family":"Khorolskaya","sequence":"first","affiliation":[{"name":"Institute of Cytology Russian Academy of Science, 194064 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria A.","family":"Perepletchikova","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Science, 194064 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6270-2866","authenticated-orcid":false,"given":"Kirill E.","family":"Zhurenkov","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Science, 194064 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2279-8514","authenticated-orcid":false,"given":"Daniel V.","family":"Kachkin","sequence":"additional","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, 199034 St. Petersburg, Russia"},{"name":"Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6203-2006","authenticated-orcid":false,"given":"Aleksandr A.","family":"Rubel","sequence":"additional","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, 199034 St. Petersburg, Russia"},{"name":"Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miralda I.","family":"Blinova","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Science, 194064 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1650-9330","authenticated-orcid":false,"given":"Natalia A.","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Science, 194064 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1093\/bmb\/ldr026","article-title":"Stem cells of the human cornea","volume":"100","year":"2011","journal-title":"Br. 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