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Although bacteriophages have been used to combat bacterial infections for over a century, a dramatic boost in phage studies has recently been observed. In the development of modern phage applications, a scientific rationale is strongly required and newly isolated phages need to be examined in detail. In this study, we present the full characterization of bacteriophages BF9, BF15, and BF17, with lytic activity against extended-spectrum \u03b2-lactamases (ESBLs)- and AmpC \u03b2-lactamases (AmpC)-producing Escherichia coli, the prevalence of which has increased significantly in livestock in recent decades, representing a great hazard to food safety and a public health risk. Comparative genomic and phylogenetic analysis indicated that BF9, BF15, and BF17 represent the genera Dhillonvirus, Tequatrovirus, and Asteriusvirus, respectively. All three phages significantly reduced in vitro growth of their bacterial host and retained the ability to lyse bacteria after preincubation at wide ranges of temperature (\u221220\u201340 \u00b0C) and pH (5\u20139). The results described herein indicate the lytic nature of BF9, BF15, and BF17, which, along with the absence of genes encoding toxins and bacterial virulence factors, represents an undoubted asset in terms of future phage application.<\/jats:p>","DOI":"10.3390\/ijms24065696","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T03:33:57Z","timestamp":1679024037000},"page":"5696","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment"],"prefix":"10.3390","volume":"24","author":[{"given":"Paulina","family":"\u015aliwka","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Food Microbiology, Wroc\u0142aw University of Environmental and Life Sciences, 50-375 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Weber-D\u0105browska","sequence":"additional","affiliation":[{"name":"Bacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroc\u0142aw, Poland"},{"name":"Phage Therapy Unit, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9556-2820","authenticated-orcid":false,"given":"Maciej","family":"\u017baczek","sequence":"additional","affiliation":[{"name":"Bacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1904-1059","authenticated-orcid":false,"given":"Marta","family":"Ku\u017ami\u0144ska-Bajor","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Microbiology, Wroc\u0142aw University of Environmental and Life Sciences, 50-375 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izabela","family":"Dusza","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Microbiology, Wroc\u0142aw University of Environmental and Life Sciences, 50-375 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6101-472X","authenticated-orcid":false,"given":"Aneta","family":"Skaradzi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Microbiology, Wroc\u0142aw University of Environmental and Life Sciences, 50-375 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"ref_1","unstructured":"ECDC European Centre for Disease Prevention and Control (2020). 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