{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:03:57Z","timestamp":1784887437127,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Joint Institute for Nuclear Research (JINR), project \u201cBIOPHOTONCS\u201d","award":["theme # 1133-2018\/2023"],"award-info":[{"award-number":["theme # 1133-2018\/2023"]}]},{"name":"Joint Institute for Nuclear Research (JINR), project \u201cBIOPHOTONCS\u201d","award":["# 178, item 11, 2022"],"award-info":[{"award-number":["# 178, item 11, 2022"]}]},{"name":"JINR and University of Belgrade","award":["theme # 1133-2018\/2023"],"award-info":[{"award-number":["theme # 1133-2018\/2023"]}]},{"name":"JINR and University of Belgrade","award":["# 178, item 11, 2022"],"award-info":[{"award-number":["# 178, item 11, 2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Neutrophils release decondensed chromatin or extracellular traps (NETs) in response to various physiological and pharmacological stimuli. Apart from host defensive functions, NETs play an essential role in the pathogenesis of various autoimmune, inflammatory, and malignant diseases. In recent years, studies have been performed on photo-induced NET formation, mainly activated by UV radiation. Understanding the mechanisms of NET release under the influence of UV and visible light is important to control the consequences of the damaging effects of electromagnetic radiation. Raman spectroscopy was applied to record characteristic Raman frequencies of various reactive oxygen species (ROS) and low-frequency lattice vibrational modes for citrulline. NETosis was induced by irradiation with wavelength-switchable LED sources. Fluorescence microscopy was used to visualize and quantify NET release. The ability of five wavelengths of radiation, from UV-A to red light, to induce NETosis was investigated at three different energy doses. We demonstrated, for the first time, that NET formation is activated not only by UV-A but also by three spectra of visible light: blue, green, and orange, in a dose-dependent manner. Using inhibitory analysis, we established that light-induced NETosis proceeds through NADPH oxidase and PAD4. The development of new drugs designed to suppress NETosis, especially when induced by exposure to intense UV and visible light, can help to mitigate light-induced photoaging and other damaging effects of electromagnetic radiation.<\/jats:p>","DOI":"10.3390\/ijms24065770","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T04:33:31Z","timestamp":1679286811000},"page":"5770","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Radiation from UV-A to Red Light Induces ROS-Dependent Release of Neutrophil Extracellular Traps"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8755-0747","authenticated-orcid":false,"given":"Grigory","family":"Arzumanyan","sequence":"first","affiliation":[{"name":"Frank Laboratory of Neutron Physics, Department of Raman Spectroscopy, Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7503-9362","authenticated-orcid":false,"given":"Kahramon","family":"Mamatkulov","sequence":"additional","affiliation":[{"name":"Frank Laboratory of Neutron Physics, Department of Raman Spectroscopy, Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yersultan","family":"Arynbek","sequence":"additional","affiliation":[{"name":"Frank Laboratory of Neutron Physics, Department of Raman Spectroscopy, Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Russia"},{"name":"Institute of Nuclear Physics, Ibragimov 1, 050032 Almaty, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darya","family":"Zakrytnaya","sequence":"additional","affiliation":[{"name":"Frank Laboratory of Neutron Physics, Department of Raman Spectroscopy, Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3382-4287","authenticated-orcid":false,"given":"Anka","family":"Jevremovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-9338","authenticated-orcid":false,"given":"Nina","family":"Vorobjeva","sequence":"additional","affiliation":[{"name":"Department of Immunology, Biology Faculty, Lomonosov Moscow State University, Lenin Hills 1\/12, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/jlb.59.2.229","article-title":"Rapid killing of human neutrophils by the potent activator phorbol 12-myristate 13-acetate (PMA) accompanied by changes different from typical apoptosis or necrosis","volume":"59","author":"Takei","year":"1996","journal-title":"J. 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