{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:29:22Z","timestamp":1792099762293,"version":"4.4.0"},"reference-count":51,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T00:00:00Z","timestamp":1679270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012546","name":"Chongqing Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["CSTB2022NSCQ-BHX0634"],"award-info":[{"award-number":["CSTB2022NSCQ-BHX0634"]}],"id":[{"id":"10.13039\/100012546","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012546","name":"Chongqing Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["cstc2021ycjh-bgzxm0198"],"award-info":[{"award-number":["cstc2021ycjh-bgzxm0198"]}],"id":[{"id":"10.13039\/100012546","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chongqing Talents Project","award":["CSTB2022NSCQ-BHX0634"],"award-info":[{"award-number":["CSTB2022NSCQ-BHX0634"]}]},{"name":"Chongqing Talents Project","award":["cstc2021ycjh-bgzxm0198"],"award-info":[{"award-number":["cstc2021ycjh-bgzxm0198"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mitochondrial dysfunction is considered an early event of Alzheimer disease (AD). D-ribose is a natural monosaccharide that exists in cells, especially in mitochondria, and can lead to cognitive dysfunction. However, the reason for this is unclear. Berberine (BBR) is an isoquinoline alkaloid that can target mitochondria and has great prospect in the treatment of AD. The methylation of PINK1 reinforces the burden of Alzheimer\u2019s pathology. This study explores the role of BBR and D-ribose in the mitophagy and cognitive function of AD related to DNA methylation. APP\/PS1 mice and N2a cells were treated with D-ribose, BBR, and mitophagy inhibitor Mdivi-1 to observe their effects on mitochondrial morphology, mitophagy, neuron histology, AD pathology, animal behavior, and PINK1 methylation. The results showed that D-ribose induced mitochondrial dysfunction, mitophagy damage, and cognitive impairment. However, BBR inhibition of PINK1 promoter methylation can reverse the above effects caused by D-ribose, improve mitochondrial function, and restore mitophagy through the PINK1\u2013Parkin pathway, thus reducing cognitive deficits and the burden of AD pathology. This experiment puts a new light on the mechanism of action of D-ribose in cognitive impairment and reveals new insights in the use of BBR for AD treatment.<\/jats:p>","DOI":"10.3390\/ijms24065896","type":"journal-article","created":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T03:15:03Z","timestamp":1679368503000},"page":"5896","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Berberine Rescues D-Ribose-Induced Alzheimer\u2018s Pathology via Promoting Mitophagy"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8144-5188","authenticated-orcid":false,"given":"Chuanling","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medicine, Chongqing Medical University, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zou","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China"},{"name":"Department of Neurology, Chongqing General Hospital, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinshuang","family":"Pu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China"},{"name":"Department of Neurology, Chongqing General Hospital, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyou","family":"Cai","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China"},{"name":"Department of Neurology, Chongqing General Hospital, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medicine, Chongqing Medical University, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1002\/alz.12638","article-title":"2022 Alzheimer\u2019s disease facts and figures","volume":"18","author":"Gaugler","year":"2022","journal-title":"Alzheimers Dement."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/s41398-020-01126-4","article-title":"Chronic D-ribose and D-mannose overload induce depressive\/anxiety-like behavior and spatial memory impairment in mice","volume":"11","author":"Xu","year":"2021","journal-title":"Transl. 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